Antigenic determinants in the N-terminal part of bovine encephalitogenic protein studied with the macrophage migration inhibition assay in guinea pigs. Evidence for an immunological reactivity of peptide 1–43 when tested on cells from animals injected with Freund's complete adjuvant

1973 ◽  
Vol 10 (4) ◽  
pp. 229-238
Author(s):  
H Bergstrand
Keyword(s):  
Guinea Pigs ◽  
Antigenic Determinants ◽  
Macrophage Migration ◽  
Inhibition Assay ◽  
Immunological Reactivity ◽  
Migration Inhibition ◽  
Terminal Part ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Macrophage Migration Inhibition

scholarly journals Evaluation of different immunization protocols with P. brasiliensis antigens in Guinea pigs

1992 ◽  
Vol 34 (3) ◽  
pp. 243-249
Author(s):  
Amélia Kamegasawa ◽  
Maria Tereza Rezkallah Iwasso ◽  
Rosa Viero ◽  
Marcello Franco
Keyword(s):  
Immune Response ◽  
Guinea Pigs ◽  
Protective Immunity ◽  
Migration Inhibition ◽  
Freund’S Complete Adjuvant ◽  
Future Studies ◽  
Freund's Complete Adjuvant ◽  
Particulate Antigen ◽  
Long Time ◽  
Macrophage Migration Inhibition

The objective of the present study was to develop an efficient and reproducible protocol of immunization of guinea pigs with P. brasiliensis antigens as an animal model for future studies of protective immunity mechanisms. We tested three different antigens (particulate, soluble and combined) and six protocols in the presence and absence of Freund's complete adjuvant and with different numbers of immunizing doses and variable lenght of time between the last immunizing dose and challenge. The efficacy of the immunizing protocol was evaluated by measuring the humoral and cellular anti-P. brasiliensis immune response of the animals, using immuno-diffusion, skin test and macrophage migration inhibition test. It was observed that: 1. Three immunizing doses of the antigens induced a more marked response than two doses; 2. The highest immune response was obtained with the use of Freund's complete adjuvant; 3. Animals challenged a long time (week 6) after the last immunizing dose showed good anti-P. brasiliensis immune response; 4. The particulate antigen induced the lowest immune response. The soluble and the combined antigens were equally efficient in raising good humoral and cellular anti-P. brasiliensis immune response

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