Ventoluft Polyherbal formulation modulates Freund’s Complete Adjuvant induced arthritis in experimental rats

Background: Ventoluft Polyherbal formulation composes multiple traditional medicinal plants composed of various anti-inflammatory agents. Hence, the present study aimed to investigate the effect of Ventoluft Polyherbal formulation on Freund’s complete adjuvant-induced arthritic male Wistar rats. Materials and methods: The study included thirty-six rats, containing six in each. Arthritis was induced by injecting FCA (0.1ml) in joints of the right sub-plantar region of rats in negative control and all test groups i.e. (20, 40, and 80mg/kg p.o) for 28 days except normal group. A positive control, indomethacin (10mg/kg, i.p.) was also tested for 28 days. During the treatment, paw volume was measured weekly once. At the end of the experiment, physical, hematological, and biochemical effects were evaluated. Results:Administration of Ventoluft at the doses of 20, 40, and 80 mg/kg b.w. p.o exhibited statistically significant (p < 0.001) inhibition of the paw edema and joint swelling, along with an increase in body weight, decrease in rheumatoid and spleen index factors. Conclusion: The results from hematological and biochemical parameters have shown that Ventoluft possesses potent anti-arthritic activity, Results obtained in the present study indicate that Ventoluft formulation has exhibited, dose-dependent improvement in FCA induced rheumatoid arthritis.

Effect of ethanol extract of Punica granatum L against Freund’s complete adjuvant-induced arthritis in rats

Purpose: To investigate the protective effect of ethanol extract of P. granatum against arthritis in rat model. Methods: Twenty-six adult male Wistar rats (120 - 150 g) were separated into four groups (n = 6): normal control, arthritic control and two treatment groups. With the exception of normal control group, arthritis was induced by intraplantar administration of Freund’s complete adjuvant (FCA) on the 1st day of drug administration. The arthritic control group was not treated, while the treatment groups received extract orally at 500 or 750 mg/kg for the period of 4 weeks and at the end of each week, paw volume, thermal hyperalgesia, arthritic score and mechanical nociceptive threshold were performed to assess arthritis. Biochemical indicators and inflammatory cytokines in serum were determined using standard procedures. Results: There was significant decrease in paw volume and arthritic score; paw withdrawal latency was enhanced in extract-treated groups, compared to arthritic control group (p < 0.05). Furthermore, ALT, AST and ALP levels, as well as RF and MDA activities decreased significantly with extract treatment, compared with arthritic control group (p < 0.05). Treatment with the extract attenuated the altered level of interleukin 1β (IL-1β) and TNF-α levels in arthritic rats. Histological examination showed that treatment with the extract significantly reversed histological changes induced by arthritis. Conclusion: The results reveal that the beneficial effect of ethanol extract of P. granatum against FCAinduced arthritis is due to its ability to reduce the levels of inflammatory cytokines.