A bacterial DNA-repair assay yielding a 6-billion-fold range of genotoxic potency

1984 ◽  
Vol 130 (3) ◽  
pp. 207-208
Author(s):  
S. De Flora ◽  
P. Zanacchi ◽  
A. Camoirano ◽  
C. Bennicelli ◽  
G.S. Badolati
Keyword(s):  
Dna Repair ◽  
Bacterial Dna ◽  
Genotoxic Potency

Development of T7 phage and T7 phage containing apurinic sites in an exonuclease III, endonuclease IV double mutant of Escherichia coli

1992 ◽  
Vol 70 (7) ◽  
pp. 605-608 ◽  
Author(s):  
Giselle Sanchez ◽  
Margaret D. Mamet-Bratley
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Dna Synthesis ◽  
Double Mutant ◽  
Bacteriophage T7 ◽  
Bacterial Dna ◽  
Exonuclease Iii ◽  
Repair Enzymes ◽  
Phage Dna ◽  
T7 Phage

The development of bacteriophage T7 was examined in an Escherichia coli double mutant defective for the two major apurinic, apyrimidinic endonucleases (exonuclease III and endonuclease IV, xth nfo). In cells infected with phages containing apurinic sites, the defect in repair enzymes led to a decrease of phage survival and a total absence of bacterial DNA degradation and of phage DNA synthesis. These results directly demonstrate the toxic action of apurinic sites on bacteriophage T7 at the intracellular level and its alleviation by DNA repair. In addition, untreated T7 phage unexpectedly displayed reduced plating efficiency and decreased DNA synthesis in the xth nfo double mutant.Key words: apurinic sites, DNA repair, T7 phage.

scholarly journals Multiple gene sequence analysis using genes of the bacterial DNA repair pathway

2015 ◽  
Vol 58 (3) ◽  
pp. 421-430 ◽  
Author(s):  
Miguel Rotelok Neto ◽  
Carolina Weigert Galvão ◽  
Leonardo Magalhães Cruz ◽  
Dieval Guizelini ◽  
Leilane Caline Silva ◽  
...  
Keyword(s):  
Dna Repair ◽  
Sequence Analysis ◽  
Gene Sequence ◽  
Repair Pathway ◽  
Bacterial Dna ◽  
Multiple Gene ◽  
Gene Sequence Analysis

scholarly journals Identification of a conserved 5′-dRP lyase activity in bacterial DNA repair ligase D and its potential role in base excision repair

2016 ◽  
Vol 44 (4) ◽  
pp. 1833-1844 ◽  
Author(s):  
Ana de Ory ◽  
Katja Nagler ◽  
Begoña Carrasco ◽  
Marina Raguse ◽  
Olga Zafra ◽  
...  
Keyword(s):  
Dna Repair ◽  
Base Excision Repair ◽  
Potential Role ◽  
Excision Repair ◽  
Bacterial Dna ◽  
Lyase Activity ◽  
Base Excision

scholarly journals Restriction endonucleases that resemble a component of the bacterial DNA repair machinery

2007 ◽  
Vol 63 (a1) ◽  
pp. s15-s15
Author(s):  
M. Bochtler ◽  
M. Sokolowska ◽  
M. Kaus-Drobek ◽  
H. Czapinska ◽  
R. Szczepanowski ◽  
...  
Keyword(s):  
Dna Repair ◽  
Restriction Endonucleases ◽  
Bacterial Dna
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