Micronucleus test with mouse peripheral blood erythrocytes by acridine orange supravital staining: The summary report of the 5th collaborative study by CSGMT/JEMS · MMS

1992 ◽  
Vol 278 (2-3) ◽  
pp. 83-98 ◽  
Author(s):  
The Collaborative Study Group for t
Keyword(s):  
Acridine Orange ◽  
Peripheral Blood ◽  
Micronucleus Test ◽  
Collaborative Study ◽  
Summary Report ◽  
Supravital Staining

Micronucleus test with vincristine sulfate and colchicine in peripheral blood reticulocytes of mice using acridine orange supravital staining

1992 ◽  
Vol 278 (2-3) ◽  
pp. 187-191 ◽  
Author(s):  
Kondo Yasushi ◽  
Honda Sachiko ◽  
Nakajima Madoka ◽  
Miyahana Koichi ◽  
Hayashi Mikiko ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Peripheral Blood ◽  
Micronucleus Test ◽  
Vincristine Sulfate ◽  
Supravital Staining

The micronucleus test of methyl methanesulfonate with mouse peripheral blood reticulocytes using acridine orange-coated slides

1992 ◽  
Vol 278 (2-3) ◽  
pp. 117-120 ◽  
Author(s):  
C. Sugiyama ◽  
Y. Miyamae ◽  
H. Kobayashi ◽  
Y. Fujino ◽  
M. Mori ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Peripheral Blood ◽  
Micronucleus Test ◽  
Methyl Methanesulfonate

A Preliminary Study of the Micronucleus Test by Acridine Orange Fluorescent Staining Compared with Chromosomal Aberration Test Using Fish Erythropoietic and Embryonic Cells

1992 ◽  
Vol 25 (11) ◽  
pp. 235-240 ◽  
Author(s):  
T. Ueda ◽  
M. Hayashi ◽  
Y. Ohtsuka ◽  
T. Nakamura ◽  
J. Kobayashi ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Chromosomal Aberration ◽  
Peripheral Blood ◽  
Cultured Cells ◽  
Micronucleus Test ◽  
Test Method ◽  
Embryonic Cells ◽  
Fluorescent Staining ◽  
Glass Slides ◽  
Chromosomal Aberration Test

The micronucleus test using fish peripheral blood has been introduced to assess the contamination of water with some mutagenic chemicals. The applicability of the micronucleus test erythrocytes combining acridine orange (AO) fluorescent staining to fish was evaluated as compared with the chromosomal aberration test method. Peripheral blood cells were smeared on glass slides, fixed with methanol, and stained with AO. AO fluorescence microscopy could differentiate between young and mature erythrocytes, thus only young erythrocytes could be observed. The sensitivity to detect the clastogenic effects of chemicals could be increased especially after acute treatment. Mitomycin C (MMC), a potent clastogen, was injected intraperitoneally to gold fishes and rose bitterlings at doses of 2, 4, 10, and 20 mg/kg. The mean frequencies of micronucleated young erythrocytes of three gold fishes peaked 3 days after treatment at 4 mg/kg body weight. Rose bitterlings showed maximum response of micronucleated young erythrocytes and chromosomal aberrations 2 days after treatment with 4 mg/kg of MMC. The cells from embryos of rose bitterlings and metropolitan bitterlings were used for the micronucleus and the chromosomal aberration test. The cultured cells established from fins of rose bitterlings were also used as materials of the chromosomal aberration.

Validation of the mouse peripheral blood micronucleus assay using acridine orange supravital staining with urethane

1992 ◽  
Vol 278 (2-3) ◽  
pp. 205-208 ◽  
Author(s):  
Kishi Michiko ◽  
Horiguchi Yoshiya ◽  
Watanabe Shigenobu ◽  
Hayashi Makoto
Keyword(s):  
Acridine Orange ◽  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Supravital Staining

Effects of benzene in a micronucleus test on peripheral blood utilizing acridine orange-coated slides

1992 ◽  
Vol 278 (2-3) ◽  
pp. 193-195 ◽  
Author(s):  
Hatakeyama Yoshiro ◽  
Nakajima Eiichi ◽  
Atai Hiroshi ◽  
Suzuki Shuzo
Keyword(s):  
Acridine Orange ◽  
Peripheral Blood ◽  
Micronucleus Test

The micronucleus assay with peripheral blood reticulocytes by acridine orange supravital staining with 1-β-d-arabinofuranosylcytosine

1992 ◽  
Vol 278 (2-3) ◽  
pp. 131-137 ◽  
Author(s):  
Iwakura Keiko ◽  
Tamura Hironobu ◽  
Matsumoto Akiko ◽  
Ajimi Syozo ◽  
Ogura Syozo ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Supravital Staining
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