Alpha-naphthyl acetate esterase activity is not a specific marker for ovine T lymphocytes

1983 ◽  
Vol 4 (4) ◽  
pp. 505-512 ◽  
Author(s):  
R.J. Dixon ◽  
K.M. Moriarty
Keyword(s):  
T Lymphocytes ◽  
Esterase Activity ◽  
Specific Marker ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

scholarly journals Ultrastructural cytochemical and autoradiographic demonstration of nonspecific esterase(s) in guinea pig basophils.

1987 ◽  
Vol 35 (3) ◽  
pp. 351-360 ◽  
Author(s):  
A M Dvorak ◽  
R A Monahan-Earley ◽  
H F Dvorak ◽  
S J Galli
Keyword(s):  
Cell Surface ◽  
Guinea Pig ◽  
Esterase Activity ◽  
Plasma Membranes ◽  
Nonspecific Esterase ◽  
Lipid Bodies ◽  
Cytoplasmic Granules ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

We used ultrastructural autoradiographic and cytochemical methods to localize esterase activities in unstimulated guinea pig basophils and in basophils undergoing degranulation or recovery from degranulation. We used tritium-labeled diisopropylfluorophosphate (DFP) as a probe for serine enzymes and localized this probe by ultrastructural autoradiography to cytoplasmic granules of immature or mature unstimulated basophils, as well as to granules released by degranulating basophils. Ultrastructural cytochemistry using alpha naphthyl acetate (ANA) as substrate localized nonspecific esterase activity to extruded granules, either within the interiors of degranulation sacs or within granules completely separated from degranulating basophils. Extruded granules retained their esterase activity for as long as 24 hr after antigen-induced degranulation. The plasma membranes of unstimulated or degranulating basophils, as well as of basophils recovering from degranulation, displayed prominent cell surface ANA esterase ectoenzyme activity. Lipid bodies, organelles present in the cytoplasm of both control and recovering basophils, were also alpha naphthyl acetate esterase (ANAE)-positive. Thus, cytochemical and autoradiographic techniques localized esterase and/or [3H]-DFP-binding activities to cytoplasmic granules, lipid bodies, and cell surface of basophils, and these enzyme activities persisted during both degranulation and recovery from degranulation.

scholarly journals Acid alpha-naphthyl acetate esterase activity in stored buffy coat smears.

1980 ◽  
Vol 28 (2) ◽  
pp. 181-182 ◽  
Author(s):  
R Giorno ◽  
S Beverly
Keyword(s):  
Esterase Activity ◽  
Buffy Coat ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
The Stability ◽  
Naphthyl Acetate

The stability of acid alpha-naphthyl acetate esterase activity in smears of buffy coats was studied. Fixed smears may be stored up to 20 days at 25 degrees C. Unfixed smears may be stored up to 24 hr at 25 degrees C before there is a significant decrease in activity. In constant, fixed or unfixed smears held at -10 degrees C begin to lose activity within 24 hr.

scholarly journals Effect of flumequine on peripheral blood leukocytes, alpha naphthyl acetate esterase activity and mitogenic lymphocyte response

2018 ◽  
Vol 74 (10) ◽  
pp. 653-657
Author(s):  
ANTONINA SOPIŃSKA ◽  
ANNA GROCHOŁA ◽  
MONIKA ROCZEŃ-KARCZMARZ ◽  
KRZYSZTOF TOMCZUK
Keyword(s):  
Peripheral Blood ◽  
Esterase Activity ◽  
Peripheral Blood Leukocytes ◽  
Con A ◽  
Blood Leukocytes ◽  
Group I ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Group Ii ◽  
Naphthyl Acetate

The aim of this study was to evaluate the effect of flumequine on the percentage of peripheral blood leukocytes of carp, alpha naphthyl acetate esterase (ANAE) activity and proliferative activity of lymphocytes stimulated with concanavalin A (Con A) and lipopolysaccharide (LPS). Flumequine was administered to 40 carp, weighing 150 ± 10 g, at a dose of 12 mg/kg, once (group I) and four times, every 2 days (group II). Among white blood cells in flumequine, treated fish (group II) observed a decrease in percentage of lymphocytes and an increase of neutrophiles. Identification of the ANAE esterase activity in fish lymphocytes of the control group showed the advantage of positive cells over the negative ones and amounted to 62.65 ± 3.22 %. After administration of flumequine in group II fish, this value decreased to 44.75 ± 3.70 %. The present study clearly demonstrated that both Con A and LPS induced lymphoid cell proliferation in vitro in group I. There was an increase in activity after stimulation of LPS and its reduction after Con A in group II. This indicates a stimulating effect of flumequine on B lymphocytes. The results of this study are not conclusive as to the positive effect of the drug on the immune system of fish and indicate the need for caution in its use. .

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