Partial characterization of circulating macromolecular β2-microglobulin complexes by density gradient ultracentrifugation and page-blotting

1983 ◽  
Vol 7 (2) ◽  
pp. 61-64 ◽  
Author(s):  
A. Falus ◽  
J.M. Rasmussen ◽  
S.-E. Svehag
Keyword(s):  
Density Gradient ◽  
Partial Characterization ◽  
Density Gradient Ultracentrifugation ◽  
Β2 Microglobulin

scholarly journals CHARACTERIZATION OF IMMUNOGLOBULIN STRUCTURES FROM THE SURFACE OF CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

1971 ◽  
Vol 134 (1) ◽  
pp. 265-280 ◽  
Author(s):  
Trond Eskeland ◽  
Eva Klein ◽  
Masaharu Inoue ◽  
Bo Johansson
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Density Gradient ◽  
Sucrose Density Gradient ◽  
Lymphocytic Leukemia ◽  
Leukemia Cells ◽  
Density Gradient Ultracentrifugation ◽  
Freezing And Thawing ◽  
Passive Hemagglutination

Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultracentrifugation, which was performed to remove the membranes from the supernatant, sedimented only half the amount of the immunoglobulin structures. By sucrose density gradient ultracentrifugation and Sephadex G-200 filtration, the unsedimented immunoglobulin structures were shown to consist of 7S IgM and free kappa chains. About 80,000 7S IgM molecules were calculated to be present on each cell. The amount of kappa chains not associated with IgM was estimated to be equal to the amount of kappa chains in IgM. Inhibition of passive hemagglutination was used to detect and quantitate the immunoglobulin structures.

scholarly journals Multiple-parameter profiling of density gradient ultracentrifugation for characterization of empty and full capsid distribution in AAV preparations

2021 ◽  
Vol 7 (2) ◽  
pp. 161-169
Author(s):  
Sebastijan Peljhan ◽  
Maja Štokelj ◽  
Sara Drmota Prebil ◽  
Pete Gagnon ◽  
Aleš Štrancar
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Multiple Parameter

scholarly journals Characterization of Maternal Isoagglutinins in ABO Hemolytic Disease of the Newborn

Blood ◽  
1966 ◽  
Vol 27 (1) ◽  
pp. 17-37 ◽  
Author(s):  
SUSIE W. FONG ◽  
ARLENE NUCKTON ◽  
H. H. FUDENBERG
Keyword(s):  
Density Gradient ◽  
Blood Group ◽  
Density Gradient Ultracentrifugation ◽  
Hemolytic Disease ◽  
Test Systems ◽  
Serum Fractions ◽  
Diethylaminoethyl Cellulose ◽  
Abo Hemolytic Disease

Abstract Sera of group O mothers of infants with and without ABO hemolytic disease of the newborn were fractionated by diethylaminoethyl-cellulose chromatography and density gradient ultracentrifugation. Whole sera and serum fractions were evaluated for activity by various test systems currently used for the antenatal prediction of ABO hemolytic disease of the newborn. The results of these studies did not show any single serologic property that would unequivocally diflerentiate between the isoantibodies of mothers of affected infants and mothers of normal infants of incompatible blood group.

Characterization of chick serum lipoproteins isolated by density gradient ultracentrifugation

1992 ◽  
Vol 100 (1) ◽  
pp. 19-22 ◽  
Author(s):  
F. Rodriguez-vico ◽  
J. M. Lopez ◽  
M. Castillo ◽  
M. F. Zafra ◽  
E. Garcia-peregrin
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Serum Lipoproteins

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte
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