scholarly journals Characterization of Maternal Isoagglutinins in ABO Hemolytic Disease of the Newborn

Blood ◽  
1966 ◽  
Vol 27 (1) ◽  
pp. 17-37 ◽  
Author(s):  
SUSIE W. FONG ◽  
ARLENE NUCKTON ◽  
H. H. FUDENBERG
Keyword(s):  
Density Gradient ◽  
Blood Group ◽  
Density Gradient Ultracentrifugation ◽  
Hemolytic Disease ◽  
Test Systems ◽  
Serum Fractions ◽  
Diethylaminoethyl Cellulose ◽  
Abo Hemolytic Disease

Abstract Sera of group O mothers of infants with and without ABO hemolytic disease of the newborn were fractionated by diethylaminoethyl-cellulose chromatography and density gradient ultracentrifugation. Whole sera and serum fractions were evaluated for activity by various test systems currently used for the antenatal prediction of ABO hemolytic disease of the newborn. The results of these studies did not show any single serologic property that would unequivocally diflerentiate between the isoantibodies of mothers of affected infants and mothers of normal infants of incompatible blood group.

scholarly journals The macromolecular properties of blood-group-specific glycoproteins. Characterization of a series of fractions obtained by density-gradient ultracentrifugation

1974 ◽  
Vol 143 (3) ◽  
pp. 669-679 ◽  
Author(s):  
K. Ramakrishnan Bhaskar ◽  
J. Michael Creeth
Keyword(s):  
Density Gradient ◽  
Blood Group ◽  
Buoyant Density ◽  
Physicochemical Characterization ◽  
Cyst Fluid ◽  
Equilibrium Density ◽  
Density Gradient Ultracentrifugation ◽  
Molecular Weights ◽  
Molar Ratios ◽  
Blood Group Substances

1. Equilibrium density-gradient ultracentrifugation in caesium salts was used in two stages in the isolation and subfractionation of the glycoprotein component from a human ovarian-cyst fluid. The eight main subfractions thus obtained were the subject of detailed physicochemical characterization. 2. The fractions were unimodal in buoyant-density distribution, but had discrete ρ0 values ranging from 1.31 to 1.35. 3. Weight-average molecular weights and sedimentation coefficients decreased regularly with decreasing density of the fraction, whereas the partial specific volumes and selective solvation parameters increased. The latter behaviour correlates well with the increasing peptide content of the lighter fractions. 4. The fractions exhibited a range of analytical composition, although all were within the limits previously observed for blood-group substances of Lea specificity. All fractions had approximately equal Lea activity. The peptide content varied systematically from 7% for the densest fraction to 15% for the lightest, but the relative distributions of the amino acids remained essentially constant throughout the series. In particular, serine plus threonine plus proline made up about 50% of the peptide content of all the fractions. Fucose, galactose and N-acetylglucosamine contents decreased with increasing peptide content of the fractions, but N-acetylgalactosamine and sialic acid exhibited the opposite trend. Molar ratios of N-acetylgalactosamine to the sum of serine and threonine remained essentially constant at 0.8–0.9, implying a high degree of glycosylation of all the molecules, but the ratio of N-acetylglucosamine to N-acetylgalactosamine decreased steadily with increasing peptide content, suggesting the presence of oligosaccharide side chains of various lengths. The results are discussed in terms of the accepted structure of glycoprotein molecules. 5. Experiments on the glycoproteins extracted with phenol from the same cyst fluid have confirmed that equilibrium centrifugation in caesium salts does not remove any non-covalently bound protein nor cause any changes in the tertiary structures of these glycoprotein molecules.

Treatment of ABO Hemolytic Disease with Synthetic Blood Group Trisaccharides

Vox Sanguinis ◽  
1994 ◽  
Vol 66 (3) ◽  
pp. 194-199 ◽  
Author(s):  
Egidio L. Romano ◽  
Andres Soyano ◽  
Ramón F. Montaño ◽  
Murray Ratcliffe ◽  
Marilyn Olson ◽  
...  
Keyword(s):  
Blood Group ◽  
Hemolytic Disease ◽  
Abo Hemolytic Disease

scholarly journals CHARACTERIZATION OF IMMUNOGLOBULIN STRUCTURES FROM THE SURFACE OF CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

1971 ◽  
Vol 134 (1) ◽  
pp. 265-280 ◽  
Author(s):  
Trond Eskeland ◽  
Eva Klein ◽  
Masaharu Inoue ◽  
Bo Johansson
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Density Gradient ◽  
Sucrose Density Gradient ◽  
Lymphocytic Leukemia ◽  
Leukemia Cells ◽  
Density Gradient Ultracentrifugation ◽  
Freezing And Thawing ◽  
Passive Hemagglutination

Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultracentrifugation, which was performed to remove the membranes from the supernatant, sedimented only half the amount of the immunoglobulin structures. By sucrose density gradient ultracentrifugation and Sephadex G-200 filtration, the unsedimented immunoglobulin structures were shown to consist of 7S IgM and free kappa chains. About 80,000 7S IgM molecules were calculated to be present on each cell. The amount of kappa chains not associated with IgM was estimated to be equal to the amount of kappa chains in IgM. Inhibition of passive hemagglutination was used to detect and quantitate the immunoglobulin structures.

Treatment of ABO Hemolytic Disease with Synthetic Blood Group Trisaccharides

Vox Sanguinis ◽  
1994 ◽  
Vol 66 (3) ◽  
pp. 194-199 ◽  
Author(s):  
Egidio L. Romano ◽  
Andres Soyano ◽  
Ramón F. Montaño ◽  
Murray Ratcliffe ◽  
Marilyn Olson ◽  
...  
Keyword(s):  
Blood Group ◽  
Hemolytic Disease ◽  
Abo Hemolytic Disease

scholarly journals Multiple-parameter profiling of density gradient ultracentrifugation for characterization of empty and full capsid distribution in AAV preparations

2021 ◽  
Vol 7 (2) ◽  
pp. 161-169
Author(s):  
Sebastijan Peljhan ◽  
Maja Štokelj ◽  
Sara Drmota Prebil ◽  
Pete Gagnon ◽  
Aleš Štrancar
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Multiple Parameter

scholarly journals Estimating the Risk of ABO Hemolytic Disease of the Newborn in Lagos

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Alani Sulaimon Akanmu ◽  
Olufemi Abiola Oyedeji ◽  
Titilope Adenike Adeyemo ◽  
Ann Abiola Ogbenna
Keyword(s):  
Blood Group ◽  
Teaching Hospital ◽  
Blood Donors ◽  
University Teaching ◽  
Abo Blood Group ◽  
Hemolytic Disease ◽  
Gene Frequencies ◽  
Abo Incompatibility ◽  
Population Prevalence ◽  
Abo Hemolytic Disease

Background. ABO hemolytic disease of the newborn is the most common hemolytic consequence of maternofetal blood group incompatibility restricted mostly to non-group-O babies of group O mothers with immune anti-A or anti-B antibodies. Aim. We estimated the risk of ABO HDN with view to determining need for routine screening for ABO incompatibility between mother and fetus. Materials and Methods. Prevalence of ABO blood group phenotypes in blood donors at the donor clinic of the Lagos University Teaching Hospital and arithmetic methods were used to determine population prevalence of ABO genes. We then estimated proportion of pregnancies of group O mothers carrying a non-group-O baby and the risk that maternofetal ABO incompatibility will cause clinical ABO HDN. Results. Blood from 9138 donors was ABO typed. 54.3%, 23%, 19.4%, and 3.3% were blood groups O, A, B, and AB, respectively. Calculated gene frequencies were 0.1416, 0.1209, and 0.7375 for A, B, and O genes, respectively. It was estimated that 14.3% of deliveries will result in a blood group O woman giving birth to a child who is non-group-O. Approximately 4.3% of deliveries are likely to suffer ABO HDN with 2.7% prone to suffer from moderately severe to severe hemolysis.

Characterization of chick serum lipoproteins isolated by density gradient ultracentrifugation

1992 ◽  
Vol 100 (1) ◽  
pp. 19-22 ◽  
Author(s):  
F. Rodriguez-vico ◽  
J. M. Lopez ◽  
M. Castillo ◽  
M. F. Zafra ◽  
E. Garcia-peregrin
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Serum Lipoproteins
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