scholarly journals Multiple-parameter profiling of density gradient ultracentrifugation for characterization of empty and full capsid distribution in AAV preparations

2021 ◽  
Vol 7 (2) ◽  
pp. 161-169
Author(s):  
Sebastijan Peljhan ◽  
Maja Štokelj ◽  
Sara Drmota Prebil ◽  
Pete Gagnon ◽  
Aleš Štrancar
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Multiple Parameter

scholarly journals CHARACTERIZATION OF IMMUNOGLOBULIN STRUCTURES FROM THE SURFACE OF CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

1971 ◽  
Vol 134 (1) ◽  
pp. 265-280 ◽  
Author(s):  
Trond Eskeland ◽  
Eva Klein ◽  
Masaharu Inoue ◽  
Bo Johansson
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Density Gradient ◽  
Sucrose Density Gradient ◽  
Lymphocytic Leukemia ◽  
Leukemia Cells ◽  
Density Gradient Ultracentrifugation ◽  
Freezing And Thawing ◽  
Passive Hemagglutination

Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultracentrifugation, which was performed to remove the membranes from the supernatant, sedimented only half the amount of the immunoglobulin structures. By sucrose density gradient ultracentrifugation and Sephadex G-200 filtration, the unsedimented immunoglobulin structures were shown to consist of 7S IgM and free kappa chains. About 80,000 7S IgM molecules were calculated to be present on each cell. The amount of kappa chains not associated with IgM was estimated to be equal to the amount of kappa chains in IgM. Inhibition of passive hemagglutination was used to detect and quantitate the immunoglobulin structures.

scholarly journals Characterization of Maternal Isoagglutinins in ABO Hemolytic Disease of the Newborn

Blood ◽  
1966 ◽  
Vol 27 (1) ◽  
pp. 17-37 ◽  
Author(s):  
SUSIE W. FONG ◽  
ARLENE NUCKTON ◽  
H. H. FUDENBERG
Keyword(s):  
Density Gradient ◽  
Blood Group ◽  
Density Gradient Ultracentrifugation ◽  
Hemolytic Disease ◽  
Test Systems ◽  
Serum Fractions ◽  
Diethylaminoethyl Cellulose ◽  
Abo Hemolytic Disease

Abstract Sera of group O mothers of infants with and without ABO hemolytic disease of the newborn were fractionated by diethylaminoethyl-cellulose chromatography and density gradient ultracentrifugation. Whole sera and serum fractions were evaluated for activity by various test systems currently used for the antenatal prediction of ABO hemolytic disease of the newborn. The results of these studies did not show any single serologic property that would unequivocally diflerentiate between the isoantibodies of mothers of affected infants and mothers of normal infants of incompatible blood group.

Characterization of chick serum lipoproteins isolated by density gradient ultracentrifugation

1992 ◽  
Vol 100 (1) ◽  
pp. 19-22 ◽  
Author(s):  
F. Rodriguez-vico ◽  
J. M. Lopez ◽  
M. Castillo ◽  
M. F. Zafra ◽  
E. Garcia-peregrin
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Serum Lipoproteins

scholarly journals Isolation of human synovial-fluid hyaluronate by density-gradient ultracentrifugation and evaluation of its protein content

1972 ◽  
Vol 126 (5) ◽  
pp. 1073-1080 ◽  
Author(s):  
Irwin Scher ◽  
David Hamerman
Keyword(s):  
Synovial Fluid ◽  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Proteolytic Digestion ◽  
Peptide Fragments ◽  
Guanidinium Chloride ◽  
Caesium Chloride ◽  
Normal Human ◽  
Protein Moiety ◽  
Human Synovial Fluid

1. A compound of hyaluronate and protein, called hyaluronate–protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate–protein was labelled with [125I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety (125I counts) and hyaluronate (hexuronate) sedimented together in the middle of the gradient. (b) The labelled hyaluronate–protein was treated with trypsin, and ultracentrifugation showed that peptide fragments (125I counts) were dispersed throughout the gradient, indicating proteolytic digestion. Hyaluronate sedimented in the middle of the gradient. (c) The labelled hyaluronate–protein was digested with streptococcal hyaluronidase, and ultracentrifugation showed that hyaluronate fragments were dispersed throughout the gradient, indicating digestion of the polysaccharide. The protein moiety, without attached hyaluronate, now sedimented at the top of the gradient. (d) Ultracentrifugation of labelled hyaluronate–protein in 4m-guanidinium chloride showed that protein and hyaluronate sedimented together. 3. These studies confirm that hyaluronate is combined with a small quantity of protein in normal human synovial fluid. A mild method for the rapid isolation of hyaluronate–protein in good yield is described.

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