Isolation and Characterization of Lipoprotein Profiles in Newborns by Density Gradient Ultracentrifugation

Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultracentrifugation, which was performed to remove the membranes from the supernatant, sedimented only half the amount of the immunoglobulin structures. By sucrose density gradient ultracentrifugation and Sephadex G-200 filtration, the unsedimented immunoglobulin structures were shown to consist of 7S IgM and free kappa chains. About 80,000 7S IgM molecules were calculated to be present on each cell. The amount of kappa chains not associated with IgM was estimated to be equal to the amount of kappa chains in IgM. Inhibition of passive hemagglutination was used to detect and quantitate the immunoglobulin structures.

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Isolation and Characterization of Mesenchymal Stem Cells from the Fat Layer on the Density Gradient Separated Bone Marrow

Stem Cells and Development ◽

10.1089/scd.2010.0572 ◽

2012 ◽

Vol 21 (2) ◽

pp. 260-272 ◽

Cited By ~ 8

Author(s):

Carmen L. Insausti ◽

Miguel Blanquer Blanquer ◽

Luis Meseguer Olmo ◽

María C. López-Martínez ◽

Xavier Férez Ruiz ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Density Gradient ◽

Isolation And Characterization

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Isolation and characterization of human cervical-mucus glycoproteins

Biochemical Journal ◽

10.1042/bj2110013 ◽

1983 ◽

Vol 211 (1) ◽

pp. 13-22 ◽

Cited By ~ 129

Author(s):

I Carlstedt ◽

H Lindgren ◽

J K Sheehan ◽

U Ulmsten ◽

L Wingerup

Keyword(s):

Density Gradient ◽

Proteinase Inhibitors ◽

Density Gradient Centrifugation ◽

Gradient Centrifugation ◽

Disc Electrophoresis ◽

Guanidinium Chloride ◽

Molecular Weights ◽

Isolation And Characterization ◽

Mucus Glycoproteins

Mucus glycoproteins (mucins) were extracted from human cervical pregnancy mucus by 6 M-guanidinium chloride in the presence of proteinase inhibitors. Purification was subsequently achieved by isopycnic density-gradient centrifugation in CsCl/ guanidinium chloride gradients. The purified macromolecules represented approx. 85% of the total and were devoid of nucleic acids and proteins, as judged by analytical density-gradient centrifugation, disc electrophoresis and u.v. spectroscopy. Sedimentation-velocity centrifugation revealed a single unimodal peak with S20,W 50.1S in 0.2M-NaCl and 37.0S in 6 M-guanidinium chloride. Molecular weights obtained by light-scattering were 9.7 × 10(6) and 5.9 × 10(6) in 0.2M-NaCl and 6 M-guanidinium chloride respectively. The chemical analyses were typical of those of epithelial mucins. The macromolecules contained approx. 20% (w/w) of protein, and 65% (w/w) was accounted for as carbohydrate. Serine and threonine constituted 32 mol/100 mol and proline 10 mol/100 mol of the amino acids. The major sugars found were N-acetylglucosamine (12.8%), N-acetylgalactosamine (9.7%), galactose (18.7%), sialic acid (15.0%) and fucose (7.5%).

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Multiple-parameter profiling of density gradient ultracentrifugation for characterization of empty and full capsid distribution in AAV preparations

Cell and Gene Therapy Insights ◽

10.18609/cgti.2021.039 ◽

2021 ◽

Vol 7 (2) ◽

pp. 161-169

Author(s):

Sebastijan Peljhan ◽

Maja Štokelj ◽

Sara Drmota Prebil ◽

Pete Gagnon ◽

Aleš Štrancar

Keyword(s):

Density Gradient ◽

Density Gradient Ultracentrifugation ◽

Multiple Parameter

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Characterization of Maternal Isoagglutinins in ABO Hemolytic Disease of the Newborn

Blood ◽

10.1182/blood.v27.1.17.17 ◽

1966 ◽

Vol 27 (1) ◽

pp. 17-37 ◽

Cited By ~ 12

Author(s):

SUSIE W. FONG ◽

ARLENE NUCKTON ◽

H. H. FUDENBERG

Keyword(s):

Density Gradient ◽

Blood Group ◽

Density Gradient Ultracentrifugation ◽

Hemolytic Disease ◽

Test Systems ◽

Serum Fractions ◽

Diethylaminoethyl Cellulose ◽

Abo Hemolytic Disease

Abstract Sera of group O mothers of infants with and without ABO hemolytic disease of the newborn were fractionated by diethylaminoethyl-cellulose chromatography and density gradient ultracentrifugation. Whole sera and serum fractions were evaluated for activity by various test systems currently used for the antenatal prediction of ABO hemolytic disease of the newborn. The results of these studies did not show any single serologic property that would unequivocally diflerentiate between the isoantibodies of mothers of affected infants and mothers of normal infants of incompatible blood group.

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