Characterization of chick serum lipoproteins isolated by density gradient ultracentrifugation

1992 ◽  
Vol 100 (1) ◽  
pp. 19-22 ◽  
Author(s):  
F. Rodriguez-vico ◽  
J. M. Lopez ◽  
M. Castillo ◽  
M. F. Zafra ◽  
E. Garcia-peregrin
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Serum Lipoproteins

scholarly journals Change of Serum Lipoproteins Isolated by Isopycnic Density Gradient Ultracentrifugation in Premenopausal Females

1983 ◽  
Vol 11 (2) ◽  
pp. 311-321
Author(s):  
Takao MAEDA ◽  
Susumu YUKAWA ◽  
Keiji MIMURA ◽  
Keizo KAWANO ◽  
Toshihiko MIYAI ◽  
...  
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Serum Lipoproteins

scholarly journals CHARACTERIZATION OF IMMUNOGLOBULIN STRUCTURES FROM THE SURFACE OF CHRONIC LYMPHOCYTIC LEUKEMIA CELLS

1971 ◽  
Vol 134 (1) ◽  
pp. 265-280 ◽  
Author(s):  
Trond Eskeland ◽  
Eva Klein ◽  
Masaharu Inoue ◽  
Bo Johansson
Keyword(s):  
Chronic Lymphocytic Leukemia ◽  
Density Gradient ◽  
Sucrose Density Gradient ◽  
Lymphocytic Leukemia ◽  
Leukemia Cells ◽  
Density Gradient Ultracentrifugation ◽  
Freezing And Thawing ◽  
Passive Hemagglutination

Chronic lymphocytic leukemia cells with relatively large amounts of mu and kappa immunoglobulin structures on the surface, and apparently very small amounts intracellularly, were subjected to homogenization or washing after freezing and thawing. After a light centrifugation, which sedimented the nuclei and unbroken cells, most of the immunoglobulin structures were found in the supernatant. Ultracentrifugation, which was performed to remove the membranes from the supernatant, sedimented only half the amount of the immunoglobulin structures. By sucrose density gradient ultracentrifugation and Sephadex G-200 filtration, the unsedimented immunoglobulin structures were shown to consist of 7S IgM and free kappa chains. About 80,000 7S IgM molecules were calculated to be present on each cell. The amount of kappa chains not associated with IgM was estimated to be equal to the amount of kappa chains in IgM. Inhibition of passive hemagglutination was used to detect and quantitate the immunoglobulin structures.

scholarly journals Multiple-parameter profiling of density gradient ultracentrifugation for characterization of empty and full capsid distribution in AAV preparations

2021 ◽  
Vol 7 (2) ◽  
pp. 161-169
Author(s):  
Sebastijan Peljhan ◽  
Maja Štokelj ◽  
Sara Drmota Prebil ◽  
Pete Gagnon ◽  
Aleš Štrancar
Keyword(s):  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Multiple Parameter
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