Isolation and characterization of DNA-binding proteins from the cyanobacterium Synechococcus sp. PCC 7002 (Agmenellum quadruplicatum) and from spinach chloroplasts

1989 ◽  
Vol 1007 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Gilles Crevel ◽  
Bernard Laine ◽  
Pierre Sautière ◽  
Colette Galleron
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Spinach Chloroplasts ◽  
Isolation And Characterization ◽  
Agmenellum Quadruplicatum ◽  
Synechococcus Sp

scholarly journals Accessibility of DNA-epoxycellulose to sequence-specific DNA-binding proteins

1984 ◽  
Vol 217 (1) ◽  
pp. 331-334 ◽  
Author(s):  
P A Lazo
Keyword(s):  
Dna Binding ◽  
Base Composition ◽  
Binding Proteins ◽  
High Efficiency ◽  
Restriction Analysis ◽  
Dna Binding Proteins ◽  
Isolation And Characterization ◽  
Base Sequences ◽  
Lambda Dna

Phage lambda DNA was covalently coupled to epoxy-activated cellulose to form a stable DNA-cellulose matrix for affinity chromatography of sequence-specific DNA-binding proteins. The accessibility of three specific six-base sequences, GGATCC (BamHI), GAATTC (EcoRI) and AAGCTT (HindIII) was studied quantitatively and qualitatively by restriction analysis followed by labelling of their recessed ends. All sites are randomly accessible. The site accessibility is variable, BamHI greater than HindIII greater than EcoRI, and within the range 20-100% depending on base composition and internal structure of the sequence. DNA-epoxycellulose, because of its high efficiency of coupling, capacity, stability and accessibility, can be of great help in the isolation and characterization of sequence-specific DNA-binding proteins.

Isolation and characterization of adenovirus type 12 DNA binding proteins

Virology ◽  
1975 ◽  
Vol 67 (1) ◽  
pp. 14-23 ◽  
Author(s):  
Brigitte Rosenwirth ◽  
Kazuko Shiroki ◽  
Arnold J. Levine ◽  
Hiroto Shimojo
Keyword(s):  
Dna Binding ◽  
Binding Proteins ◽  
Adenovirus Type ◽  
Dna Binding Proteins ◽  
Isolation And Characterization

Isolation and characterization of Z-DNA binding proteins from wheat germ

Biochemistry ◽  
1985 ◽  
Vol 24 (19) ◽  
pp. 5070-5076 ◽  
Author(s):  
Eileen M. Lafer ◽  
Rui Sousa ◽  
Barry Rosen ◽  
Anna Hsu ◽  
Alexander Rich
Keyword(s):  
Dna Binding ◽  
Wheat Germ ◽  
Binding Proteins ◽  
Dna Binding Proteins ◽  
Isolation And Characterization ◽  
Z Dna
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