Isolation and characterization of a new form of the porcine pancreatic secretory trypsin inhibitor Biochemical studies and high-resulution 1H-NMR

1982 ◽  
Vol 707 (1) ◽  
pp. 50-58 ◽  
Author(s):  
Enea Menegatti ◽  
Fabrizio Bortolotti ◽  
Lorenzo Minchiotti ◽  
Antonio De Marco
Keyword(s):  
1H Nmr ◽  
Trypsin Inhibitor ◽  
Pancreatic Secretory Trypsin Inhibitor ◽  
Isolation And Characterization ◽  
Biochemical Studies ◽  
New Form

Isolation and characterization of a trypsin inhibitor from finger millet

1983 ◽  
Vol 22 (11) ◽  
pp. 2349-2357 ◽  
Author(s):  
Nagenahally H. Manjunath ◽  
Patnagere S. Veerabhadrappa ◽  
Tumkur K. Virupaksha
Keyword(s):  
Trypsin Inhibitor ◽  
Finger Millet ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of the human inter-alpha-trypsin inhibitor heavy-chain H1 gene

1993 ◽  
Vol 218 (2) ◽  
pp. 283-291 ◽  
Author(s):  
Frederic BOST ◽  
Jeannette BOURGUIGNON ◽  
Jean-Pierre MARTIN ◽  
Richard SESBOUE ◽  
Luc THIBERVILLE ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Trypsin Inhibitor ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of piperine from the fruits of black pepper (Piper nigrum)

2014 ◽  
Vol 11 (1) ◽  
pp. 11-16 ◽  
Author(s):  
KC Saha ◽  
HP Seal ◽  
MA Noor
Keyword(s):  
1H Nmr ◽  
Column Chromatography ◽  
Mass Spectra ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Crystalline Compound ◽  
Food Preservative ◽  
Traditional Medicines ◽  
Isolation And Characterization

Black pepper (Piper nigrum) is a spice vine crop which is used as a food preservative and as an essential component in traditional medicines. The aim of this study was to extract, isolate and characterize the structure of piperine. The fruits of black pepper were extracted in ethanol and compounds present identified by TLC under iodine vapour. A compound (A2) having Rf value 0.54 was isolated by column chromatography which may be responsible for the pungency of black pepper. After recrystallization of the isolate with benzene and chloroform mixture (7:1) gave pale yellow crystals of m.p. 128~129oC (reported 130oC). The IR, 1H NMR and mass spectra of the pure crystalline compound (A2) were recorded. The mass spectra of the compound (A2) showed molecular ion peak M+· at 284.5 which was similar to that of piperine (mol. wt. 285). DOI: http://dx.doi.org/10.3329/jbau.v11i1.18197 J. Bangladesh Agril. Univ. 11(1): 11-16, 2013

Genetic and biochemical studies of asparagine-linked oligosaccharide assembly

1982 ◽  
Vol 300 (1099) ◽  
pp. 207-223 ◽  
Keyword(s):  
Protein Synthesis ◽  
Temperature Sensitive ◽  
Temperature Sensitive Mutants ◽  
Isolation And Characterization ◽  
Oligosaccharide Processing ◽  
Biochemical Studies ◽  
The Common ◽  
Specific Lipid ◽  
Temperature Sensitive Phenotype

The formation of N -glycosidic linkages of eukaryotic glycoproteins involves the assembly of a specific lipid-linked precursor oligosaccharide in the endoplasmic reticulum. This oligosaccharide is transferred from the lipid carrier to appropriate asparagine residues during protein synthesis. The protein-linked oligosaccharide then undergoes processing reactions that include both removal and addition of carbohydrate residues. In this paper we report recent studies from our laboratory on the synthesis of asparagine-linked oligosaccharides. In the first part we describe the isolation and characterization of temperature-sensitive mutants of yeast blocked at specific stages in the assembly of the lipid-linked oligosaccharide. In addition, we are using these mutants to clone the genes for the enzymes in this pathway by complementation of the temperature-sensitive phenotype. The second part deals with the topography of asparagine-linked oligosaccharide assembly. Our studies on the transmembrane movement of sugar residues during the assembly of secreted glycoproteins from cytoplasmic precursors are presented. Finally, experiments on the control of protein-linked oligosaccharide processing are described. Recent data are presented on the problem of how specific oligosaccharides are assembled from the common precursors at individual sites on glycoproteins.

Isolation and characterization of a trypsin inhibitor from the skin secretions of Kaloula pulchra hainana

Toxicon ◽  
2010 ◽  
Vol 56 (4) ◽  
pp. 502-507 ◽  
Author(s):  
Yingxia Zhang ◽  
Manchuriga Wang ◽  
Shuangshuang Wei
Keyword(s):  
Trypsin Inhibitor ◽  
Isolation And Characterization ◽  
Skin Secretions
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