A poplar by any other name … In Vitro Methods for Conservation of Plant Genetic Resources edited by John H. Dodds, Chapman & Hall, 1990. £27.50 (247 pages) ISBN 0 412 33870 X

1991 ◽  
Vol 9 (1) ◽  
pp. 332
Author(s):  
M Fay
2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Ines Van den houwe ◽  
Rachel Chase ◽  
Julie Sardos ◽  
Max Ruas ◽  
Els Kempenaers ◽  
...  

AbstractThe CGIAR genebank International Musa Germplasm Transit Centre (ITC) currently holds 1617 banana accessions from 38 countries as an in vitro collection, backed-up by a cryopreserved collection to safeguard global Musa diversity in perpetuity. The ITC also serves as a vital safety backup and transit centre for national banana genebanks and ensures that germplasm is clean of pests and diseases and freely available under the International Treaty on Plant Genetic Resources for Food and Agriculture. In more than 35 years of activity, the ITC has distributed over 18,000 banana accession samples to researchers and farmers in 113 countries. Ex situ conservation of vegetatively-propagated crops such as banana poses very particular challenges. Maintaining the ITC genebank is labor intense and costly. Efficiencies are sought through research and development of techniques on detecting viruses, the genetic integrity of accessions, and on innovative means of safeguarding banana diversity, such as conserving populations of wild species by seed banking. Although the conservation of global banana diversity is the main objective of the ITC, significant value comes from its holistic approach to better understand and promote its germplasm through numerous research activities and resources. Techniques for morphological and molecular characterization serve to identify and describe the collection, while also determining what gaps should be filled by collecting missions with national partners. The evaluation of desirable agronomic traits inherent in Musa spp. are investigated by a high-throughput phenotyping platform, which helps breeding programs to select cultivars resistant or tolerant to biotic and abiotic stresses. Genomic and bioinformatic studies of several banana wild relatives greatly enhance our understanding of Musa genetic diversity, links to important phenotypic traits and bring new methods for management of the collection. Collectively, these research activities produce enormous amounts of data that require curation and dissemination to the public. The two information systems at the ITC, Musa Genebank Management System and the Musa Germplasm Information System, serve to manage the genebank activities and to make public germplasm-related data for over 30 banana collections worldwide, respectively. By implementing the 10-year workplan set out in the Global Strategy for the Conservation and Use of Musa Genetic Resources, the network MusaNet supports Musa researchers and stakeholders, including the ITC, and most importantly, links to the world’s banana-producing countries via three regional banana networks.


Author(s):  
Jane Muthoni ◽  
Hussein Shimelis ◽  
Rob Melis

Plant genetic resources (PGRs) play an important role in agriculture, environment protection, cultural property and trade; they need to be conserved. There are two fundamental approaches for the conservation of PGRs: in situ and ex situ. In situ conservation is the conservation of ecosystems and natural habitats and the maintenance and recovery of viable populations of species in their natural surroundings. Ex situ preservation is the storage of seeds or plant materials under artificial conditions to maintain their long term viability and availability for use. Genebanks employ seed storage, field collections of living plants and in vitro storage (tissue culture or cryopreservation) for ex situ preservation of PGR. Storage of orthodox seeds, which are tolerant to low moisture content and low temperatures at appropriate temperature and humidity, is the most convenient ex situ conservation method. Plants that produce recalcitrant seeds or non-viable seeds are conserved in field genebanks as well as in-vitro in slow growth media for short-to-medium term and cryopreservation in liquid nitrogen at -1960C for long-term periods. Cryopreservation is very expensive and needs trained personnel; this could explain why this method is rarely used for conservation of plant genetic resources in most developing countries. Potato tubers are bulky and highly perishable; the crop is generally conserved as clones either in field genebanks (with annual replanting), in-vitro conservation in slow growth media for short-to-medium term and cryopreservation for long term. Field genebanks are expensive to maintain and the crop is exposed to many dangers; hence, cryopreservation is the only feasible method for long term conservation. However, given the high cost of cryopreservation, long-term conservation of potato genetic resources is poorly developed in most resource-poor countries leading to high rates of genetic erosion. This paper looks into the various methods that that can be applied to conserve potato genetic resources and the status of conservation of potatoes in major genebanks and some countries.


Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 813-817 ◽  
Author(s):  
W. M. Roca ◽  
R. Chavez ◽  
M. L. Martin ◽  
D. I. Arias ◽  
G. Mafla ◽  
...  

For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germ plasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro active gene bank where cultures are maintained under slow growth; of the few of these that exist, the cassava (Manihot) in vitro active gene bank of the Centro Internacional de Agricultura Tropical (CIAT) consists of over 4000 clones; (ii) an in vitro base gene bank, where cultures are cryopreserved; at present, none of this type exist. In vitro conservation offers a means of maintaining valuable gene combinations in a small space, protected against pest and disease attack, soil problems, and climatic changes, and with high multiplication potential. The main limitations are inability to regenerate plants of many important crops and the risk of genetic instability of cultures. A joint project of the International Board for Plant Genetic Resources and CIAT is underway to develop an in vitro based set of procedures for handling germ plasm, using cassava as a model, with culture maintenance, stability monitoring, and management data bases being considered. Results of recent work on in vitro conservation and related activities, i.e., germ-plasm collection, exchange, and characterization, will be presented and discussed, with special reference to Manihot.Key words: in vitro gene banks, cryopreservation, isozyme electrophoresis, genotype stability, Manihot esculenta Crantz.


CORD ◽  
2005 ◽  
Vol 21 (02) ◽  
pp. 34
Author(s):  
Pons Batugal

The International Coconut Genetic Resources Network (COGENT) is a global research network organized by the International Plant Genetic Resources Institute (IPGRI) in 1992 with support from member countries, the Consultative Group on International Agricultural Research (CGIAR), partner institutions, donor agencies, and by regional and international development organizations.                           In the last 12 years, COGENT has been fully operational with 38 member coconut producing countries in five regions (South Asia; Southeast and East Asia; South Pacific; Africa and the Indian Ocean; and Latin America and the Caribbean). It has successfully developed and disseminated to coconut breeders and curators worldwide the International Coconut Genetic Resources Database (CGRD). The CGRD contains characterization data and some pictures of 1,416 accessions which are conserved by national programmes in 28 sites in 23 countries. To further secure conserved germplasm, a COGENT multi-site International Coconut Genebank has been established to conserve 200 important accessions in each region. Coconut varieties with multi-purpose uses are being identified, documented and promoted. The performance of promising 38 high-yielding hybrids are being evaluated in a multilocation  trial involving four African and three Latin America/Caribbean countries to identify suitable varieties and hybrids for resource-poor farmers. Farmers’ varietal preferences in 15 countries are being evaluated. Diversity-linked income-generating activities are being used as a strategy to promote in situ and on-farm conservation and germplasm utilization have been initiated in 15 countries. Protocols for in vitro embryo culture, cryopreservation, morphometric and molecular marker-based methods for locating and characterizing diversity; pest risk assessment and germplasm health management are being developed, tested and upgraded. Strategies and techniques for farmer participatory research, collecting, characterization and ex situ and in situ conservation are being refined.   To strengthen the coconut research capability of COGENT member countries, the COGENT Secretariat and IPGRI have organized 39 country need assessment missions  and conducted 41 workshops and meetings involving 994 coconut researchers to share information and technologies, discuss issues and common problems and opportunities and how to address them; conducted 40 training courses involving 765 participants from 41 countries; supported 274  research and training/capacity building activities  in 30 countries; and led the establishment of the Global Coconut Research for Development Programme (PROCORD). IPGRI and COGENT's current priority involves the further promotion of more effective conservation and use of coconut genetic resources, both regionally and globally.


2019 ◽  
Vol 1 (4) ◽  
Author(s):  
R. K. Salgotra 1 ◽  
Manmohan Sharma 1 ◽  
Pankaj Pandotra 1

Climate change is an unequivocal fact and its impacts are already perceptible today. Climate change will profoundly alter the present conditions of agriculture in almost all countries with variable severity from region to region. On one hand unpredicted changes in climatic parameters are likely to threaten the production and productivity of economically important plant species particularly that of food crops, at the same time agriculture productivity requires significant increase to meet the expected growth in demand for food by the ever increasing world population. Conservation and sustainable use of diversity present in plant genetic resources and traditional knowledge of germplasm within and among plant species represent economic, scientific and societal value which has tendency to solve the food security problems erupt due to expanding global population. An advance made in biotechnology field such as in vitro culture technology, cryopreservation and molecular markers technology has generated significant contributions to improve the methods of conservation of rare and endangered plant genetic resources and traditional knowledge of germplasm and their valuable management in an effective way. A strategic and forward vision for conservation of plant genetic resources and traditional knowledge of germplasm and sustainable use of plant resources in the 21st century is of far reaching significance for sustainable development.


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