Production of recombinant hirudin by high cell density fed-batch cultivations of a Saccharomyces cerevisiae strain: physiological considerations during the bioprocess design

1994 ◽  
Vol 32 (3) ◽  
pp. 249-259 ◽  
Author(s):  
O. Mendoza-Vega ◽  
C. Hebert ◽  
S.W. Brown
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Fed Batch ◽  
Recombinant Hirudin ◽  
Bioprocess Design

scholarly journals Exometabolome analysis reveals hypoxia at the up-scaling of a Saccharomyces cerevisiae high-cell density fed-batch biopharmaceutical process

2014 ◽  
Vol 13 (1) ◽  
pp. 32 ◽  
Author(s):  
Zhibiao Fu ◽  
Thomas D Verderame ◽  
Julie M Leighton ◽  
Brante P Sampey ◽  
Edward R Appelbaum ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
Fed Batch ◽  
Up Scaling

Optimization of Overproducing S-Adenosyl-L-Methionine Saccharomyces Cerevisiae S-W55 Mutant Utilizing Unpolished Rice from Aging Paddy by Feeding L-Methionine

2011 ◽  
Vol 365 ◽  
pp. 445-453
Author(s):  
Wen Jun Wang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Density ◽  
Yeast Extract ◽  
High Cell Density ◽  
Fermentation Broth ◽  
Raw Material ◽  
Batch Processes ◽  
High Cell ◽  
Fed Batch ◽  
Unpolished Rice

The medium and fed-batch fermentation by Saccharomyces cerevisiae S-W55 were optimized. The unpolished rice from aging paddy was used as major nutrient source to reduce the raw material cost of SAM fermentation. The unpolished rice saccharificate (URS) and yeast extract were employed as carbon source and nitrogen source, respectively. The dosages of URS and yeast extract in the medium compositions were optimized by response surface methodology (RSM). As a result, when the fermentation was carried out under the optimal conditions for URS (51.4 g/L) and yeast extract (4.74 g/L), the SAM yield reached 2.61 g/L. Some fed-batch processes by adding L-methionine (MET) were investigated. Adding MET into the fermentation broth at one time at the time of high cell density reaching 80 g/L could get better results with the optimal SAM concentration of 5.3 g/L and biomass yield of 89.1 g/L. By feeding MET into the fermentation broth at a feeding rate of 2 g/h for 5 hours at the time of high cell density reaching 80 g/L the optimal results were reached and the maximal SAM yield and biomass density were 5.82 g/L and 90.2 g/L，respectively.

scholarly journals A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures

2010 ◽  
Vol 9 (1) ◽  
pp. 11 ◽  
Author(s):  
Mirja Krause ◽  
Kaisa Ukkonen ◽  
Tatu Haataja ◽  
Maria Ruottinen ◽  
Tuomo Glumoff ◽  
...  
Keyword(s):  
Cell Density ◽  
Recombinant Proteins ◽  
High Cell Density ◽  
High Cell ◽  
Fed Batch ◽  
Cultivation Method
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