Selection of Somatic Hybrid Plants in Nicotiana Through Fluorescence-Activated Sorting of Protoplasts

Sexual hybrid plants of Lycopersicon esculentum × L. pennellii (E × P) have been transformed and the T-DNA inserts genetically mapped. Donor protoplasts of E × P were isolated from leaves, and subsequently irradiated with 0, 5, 10 and 20 krad of a 60Co. They were then fused with suspension-derived protoplasts of S. lycopersicoides using the PEG-CA++-high pH technique. The protoplasts were cultured in medium 8E at 1.5 × 106 protoplasm/ml. Selection of heterokaryon-derived macrocalli was facilitated by the inability of irradiated donor protoplasts to divide and by plating p-calli on regeneration medium containing kanamycin - an antibiotic for which the resistance gene NPTII is part of the T-DNA. Morphological characteristics of the resulting asymmetric somatic hybrid plants indicate that 10 and 20 krad irradiation eliminates a larger port ion of the genome than does 5 krad. This has been confirmed with isozyme analysis and chromosome counts which generally show the 5 krad asymmetric somatic hybrid plants to differ little from symmetric hybrids although they contain some significant exceptions. Isozyme data reveals the 10 and 20 krad plants to have received much less of the donor genome. Exact quantification is continuing using isozyme markers, chromosome counts and cDNA probes.

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A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solatium tuberosum L.)

Theoretical and Applied Genetics ◽

10.1007/bf00222329 ◽

1992 ◽

Vol 85 (4) ◽

pp. 470-479 ◽

Cited By ~ 15

Author(s):

S. Waara ◽

L. Pijnacker ◽

M. A. Ferwerda ◽

A. Wallin ◽

T. Eriksson

Keyword(s):

Somatic Hybrid ◽

Phenotypic Characterization ◽

Hybrid Plants ◽

Solatium Tuberosum

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Molecular analysis of single copy and repetitive genes on chromosome 2 in intergeneric tomato somatic hybrid plants

Plant Molecular Biology ◽

10.1007/bf00015666 ◽

1988 ◽

Vol 11 (2) ◽

pp. 139-145 ◽

Cited By ~ 9

Author(s):

Patrick P. Moore ◽

Kenneth C. Sink

Keyword(s):

Molecular Analysis ◽

Somatic Hybrid ◽

Single Copy ◽

Chromosome 2 ◽

Hybrid Plants

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Electrofusion for the production of somatic hybrid plants of Solanum melongena L. and Solanum khasianum C.B. Clark

Plant Science ◽

10.1016/0168-9452(88)90127-6 ◽

1988 ◽

Vol 57 (3) ◽

pp. 215-223 ◽

Cited By ~ 43

Author(s):

Darasinh Sihachakr ◽

Robert Haicour ◽

Isabelle Serraf ◽

Edilberto Barrientos ◽

Catherine Herbreteau ◽

...

Keyword(s):

Somatic Hybrid ◽

Solanum Melongena ◽

Hybrid Plants ◽

Solanum Khasianum

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Production of Somatic Hybrid Plants between Japanese Bunching Onion(Allium fistulosum L.) and Bulb Onion(A. cepa L.) via Electrofusion.

Engei Gakkai zasshi ◽

10.2503/jjshs.71.623 ◽

2002 ◽

Vol 71 (5) ◽

pp. 623-631 ◽

Cited By ~ 4

Author(s):

Masahito Shimonaka ◽

Takashi Hosoki ◽

Motonori Tomita ◽

Yoshimasa Yasumuro

Keyword(s):

Somatic Hybrid ◽

Allium Fistulosum ◽

Bulb Onion ◽

Hybrid Plants ◽

Japanese Bunching Onion ◽

Bunching Onion

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Caipira sweet orange + Rangpur lime: a somatic hybrid with potential for use as rootstock in the Brazilian citrus industry

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000300026 ◽

2000 ◽

Vol 23 (3) ◽

pp. 661-665 ◽

Cited By ~ 17

Author(s):

Fernanda Januzzi Mendes-da-Glória ◽

Francisco de Assis Alves Mourão Filho ◽

Luis Eduardo Aranha Camargo ◽

Beatriz Madalena Januzzi Mendes

Keyword(s):

Somatic Hybrid ◽

Leaf Morphology ◽

Somatic Hybrids ◽

Sweet Orange ◽

Southeastern Brazil ◽

Citrus Industry ◽

Rangpur Lime ◽

Rapd Profile ◽

Hybrid Plants ◽

Regenerated Plants

Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.

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