Nitrate reductase activity changes during a culture cycle of tobacco cells: the participation of a membrane-bound form enzyme

Plant Science ◽  
1991 ◽  
Vol 79 (2) ◽  
pp. 193-204 ◽  
Author(s):  
J. Hoarau ◽  
A. Nato ◽  
D. Lavergne ◽  
V. Flipo ◽  
B. Hirel
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Tobacco Cells ◽  
Membrane Bound ◽  
Culture Cycle

scholarly journals Characterization of the membrane-bound nitrate reductase activity of aerobically grown chlorate-sensitive mutants of escherichia coli K12

FEBS Letters ◽  
1978 ◽  
Vol 95 (2) ◽  
pp. 290-294 ◽  
Author(s):  
Gérard Giordano ◽  
Alec Graham ◽  
David H. Boxer ◽  
Bruce A. Haddock ◽  
Edgard Azoulay
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Escherichia Coli K12 ◽  
Membrane Bound

scholarly journals Synthesis of cytoplasmic membrane during growth and division of Escherichia coli. Dispersive behaviour of respiratory nitrate reductase

1979 ◽  
Vol 184 (1) ◽  
pp. 45-50 ◽  
Author(s):  
E Cadenas ◽  
P B Garland
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Cytoplasmic Membrane ◽  
Reductase Activity ◽  
Selection Method ◽  
Separation Procedure ◽  
Physical Separation ◽  
Membrane Bound ◽  
Respiratory Nitrate Reductase

We have used the penicillin selection method of Autissier & Kepes [(1972) Biochimie 54, 93–101] to study the segregation of membrane-bound respiratory nitrate reductase (EC 1.9.6.1) in Escherichia coli for the three generations after cessation of nitrate reductase synthesis caused by withdrawal of nitrate from the growth medium. We also included a physical separation procedure that permitted direct assay for nitrate reductase activity among all fractions produced by the penicillin selection method. We conclude that the segregation of nitrate reductase after cell division is dispersive, and not semi-conservative as proposed by Autissier & Kepes (1972).

Molybdate-dependent expression of the periplasmic nitrate reductase in Bradyrhizobium japonicum

2005 ◽  
Vol 33 (1) ◽  
pp. 127-129
Author(s):  
N. Bonnard ◽  
A. Tresierra-Ayala ◽  
E.J. Bedmar ◽  
M.J. Delgado
Keyword(s):  
Nitrate Reductase ◽  
Transport System ◽  
Bradyrhizobium Japonicum ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Lacz Fusion ◽  
Periplasmic Nitrate Reductase ◽  
High Affinity ◽  
System A ◽  
Membrane Bound

The napEDABC genes of Bradyrhizobium japonicum encode the periplasmic nitrate reductase, an Mo-containing enzyme which catalyses the reduction of nitrate to nitrite when oxygen concentrations are limiting. In this bacterium, another set of genes, modABC, code for a high affinity ABC-type Mo transport system. A B. japonicum modA mutant has been obtained that is not capable of growing anaerobically with nitrate and lacks nitrate reductase activity. Under nitrate respiring conditions, when Mo concentrations are limiting, the B. japonicum modA mutant lacked both the 90 kDa protein corresponding to the NapA component of the periplasmic nitrate reductase, and the membrane-bound 25 kDa c-type cytochrome NapC. Regulatory studies using a napE–lacZ fusion indicated that napE expression was highly reduced in the modA mutant background when the cells were incubated anaerobically with nitrate under Mo-deficient conditions.

Nitrate Reductase Activity of Maize Hybrids and Their Parental Inbred 1

Crop Science ◽  
1966 ◽  
Vol 6 (2) ◽  
pp. 169-173 ◽  
Author(s):  
L. E. Schrader ◽  
D. M. Peterson ◽  
E. R. Leng ◽  
R. H. Hageman
Keyword(s):  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Maize Hybrids ◽  
Parental Inbred
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