scholarly journals Characterization of the membrane-bound nitrate reductase activity of aerobically grown chlorate-sensitive mutants of escherichia coli K12

FEBS Letters ◽  
1978 ◽  
Vol 95 (2) ◽  
pp. 290-294 ◽  
Author(s):  
Gérard Giordano ◽  
Alec Graham ◽  
David H. Boxer ◽  
Bruce A. Haddock ◽  
Edgard Azoulay
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Escherichia Coli K12 ◽  
Membrane Bound

scholarly journals Synthesis of cytoplasmic membrane during growth and division of Escherichia coli. Dispersive behaviour of respiratory nitrate reductase

1979 ◽  
Vol 184 (1) ◽  
pp. 45-50 ◽  
Author(s):  
E Cadenas ◽  
P B Garland
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Nitrate Reductase Activity ◽  
Cytoplasmic Membrane ◽  
Reductase Activity ◽  
Selection Method ◽  
Separation Procedure ◽  
Physical Separation ◽  
Membrane Bound ◽  
Respiratory Nitrate Reductase

We have used the penicillin selection method of Autissier & Kepes [(1972) Biochimie 54, 93–101] to study the segregation of membrane-bound respiratory nitrate reductase (EC 1.9.6.1) in Escherichia coli for the three generations after cessation of nitrate reductase synthesis caused by withdrawal of nitrate from the growth medium. We also included a physical separation procedure that permitted direct assay for nitrate reductase activity among all fractions produced by the penicillin selection method. We conclude that the segregation of nitrate reductase after cell division is dispersive, and not semi-conservative as proposed by Autissier & Kepes (1972).

scholarly journals Purification and further characterization of the second nitrate reductase of Escherichia coli K12

1990 ◽  
Vol 188 (3) ◽  
pp. 679-687 ◽  
Author(s):  
Chantal IOBBI-NIVOL ◽  
Claire-Lise SANTINI ◽  
Francis BLASCO ◽  
Gerard GIORDANO
Keyword(s):  
Escherichia Coli ◽  
Nitrate Reductase ◽  
Escherichia Coli K12

A Study on the Etiological Factors of Bilharzial Bladder Cancer in Egypt. 6. The Possible Role of Urinary Bacteria

1982 ◽  
Vol 68 (1) ◽  
pp. 23-28 ◽  
Author(s):  
Abdelbaset Anwer El-Aaser ◽  
Mahmoud Mohamed El-Merzabani ◽  
Nadia Ahmed Higgy ◽  
Abdel E. El-Habet
Keyword(s):  
Escherichia Coli ◽  
Bladder Cancer ◽  
Enzyme Activity ◽  
Nitrate Reductase ◽  
Bacterial Infection ◽  
Schistosoma Haematobium ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Glucuronidase Activity

A correlation was obtained between a positive nitrite test in urine and the severity of urinary bacterial infection. Bacteria isolated from the urine of bilharzial or bladder cancer patients were found to be rich in nitrate reductase activity. « Escherichia coli » was the most common microorganism isolated from these specimens. Urine and several urinary constituents activate bacterial nitrate reductase. β-Glucuronidase activity in the urine of patients with chronic « Schistosoma haematobium » infection and bladder cancer was measured and shown to be significantly greater than that of urine of normal control subjects. Urinary bacterial infection was shown to be the source of the increased urinary level of enzyme activity at pH 7.0.

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