A chloride- and calcium-dependent glutamate-binding protein from rat brain: Identification as a ubiquitous constituent of the inner mitochondrial membrane

1990 ◽  
Vol 16 ◽  
pp. 31
Keyword(s):  
Rat Brain ◽  
Mitochondrial Membrane ◽  
Binding Protein ◽  
Inner Mitochondrial Membrane ◽  
Calcium Dependent ◽  
Glutamate Binding

The calcium-sensitive large-conductance potassium channel (BK/MAXI K) is present in the inner mitochondrial membrane of rat brain

Neuroscience ◽  
2006 ◽  
Vol 139 (4) ◽  
pp. 1249-1261 ◽  
Author(s):  
R.M. Douglas ◽  
J.C.K. Lai ◽  
S. Bian ◽  
L. Cummins ◽  
E. Moczydlowski ◽  
...  
Keyword(s):  
Potassium Channel ◽  
Rat Brain ◽  
Mitochondrial Membrane ◽  
Inner Mitochondrial Membrane

Solubilisation of a Glutamate Binding Protein from Rat Brain

1987 ◽  
Vol 49 (1) ◽  
pp. 272-281 ◽  
Author(s):  
Donald R. Kuonen ◽  
Peter J. Roberts
Keyword(s):  
Rat Brain ◽  
Binding Protein ◽  
Glutamate Binding

scholarly journals Mitochondrial hexokinase from small-intestinal mucosa and brain

1971 ◽  
Vol 124 (3) ◽  
pp. 491-500 ◽  
Author(s):  
R. J. Mayer ◽  
G. Hübscher
Keyword(s):  
Small Intestine ◽  
Rat Brain ◽  
Mitochondrial Membrane ◽  
Type I ◽  
Small Intestinal Mucosa ◽  
Mitochondrial Enzymes ◽  
Outer Mitochondrial Membrane ◽  
Inner Mitochondrial Membrane ◽  
Hexokinase Activity ◽  
Small Intestinal

1. The submitochondrial localization of hexokinase activity in preparations of mitochondria from the small intestine of the guinea pig was studied by conventional methods. 2. Hexokinase activity in this tissue was predominantly associated with the outer mitochondrial membrane. 3. The inactivation of mitochondrial enzymes by trypsin in iso-osmotic and hypo-osmotic conditions was also used to determine the submitochondrial localization of hexokinase activity. 4. Hexokinase activity was found to be on the outside of the outer mitochondrial membrane. 5. It was shown that both type I and type II hexokinase activities are bound to the outside of the outer mitochondrial membrane. The types are present in the same ratio as that in which they occur in the cytosol of the cell. 6. Mitochondrial hexokinase from the small intestine did not show the latency phenomenon demonstrated by mitochondrial hexokinase from brain when subjected to a variety of treatments. However, hexokinase activity was solubilized from preparations of mitochondria from the small intestine by the same treatments as for mitochondrial hexokinase from brain. 7. The submitochondrial distribution of hexokinase activity in mitochondrial preparations from rat brain was determined by the trypsin inactivation method. 8. Hexokinase activity in preparations of mitochondria from rat brain was found on the outside of the outer membrane, between the mitochondrial membranes, and within the inner mitochondrial membrane. 9. Hexokinase from rat brain showed latency properties irrespective of its submitochondrial location.

Sign in / Sign up

Export Citation Format

Share Document