Brefeldin A (BFA) inhibits transcytosis in the isolated perfused rat liver (IPRL) with no effect on bile flow and bile salt secretion

Hepatology ◽  
1993 ◽  
Vol 18 (4) ◽  
pp. A294
Author(s):  
D ALVARO
Keyword(s):  
Bile Salt ◽  
Rat Liver ◽  
Bile Flow ◽  
Brefeldin A ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Salt Secretion

scholarly journals Acute effects of cholestatic and choleretic bile salts on vasopressin- and glucagon-induced hepato-biliary calcium fluxes in the perfused rat liver

1992 ◽  
Vol 283 (2) ◽  
pp. 575-581 ◽  
Author(s):  
Y Hamada ◽  
A Karjalainen ◽  
B A Setchell ◽  
J E Millard ◽  
F L Bygrave
Keyword(s):  
Bile Salt ◽  
Rat Liver ◽  
Strong Correlation ◽  
Bile Flow ◽  
Bile Salts ◽  
Perfused Rat Liver ◽  
Acute Effects ◽  
Calcium Fluxes ◽  
Principal Effect

The effects were investigated of the choleretic bile salt glycoursodeoxycholate (G-UDCA) and of the cholestatic bile salt taurochenodeoxycholate (T-CDCA) on changes in perfusate Ca2+, glucose and oxygen and in bile calcium and bile flow induced by the administration of (a) vasopressin, (b) glucagon and (c) glucagon plus vasopressin together to the perfused rat liver [Hamada, Karjalainen, Setchell, Millard & Bygrave (1992) Biochem. J. 281, 387-392]. G-UDCA itself increased the secretion of calcium in the bile several-fold, but its principal effect was to augment each of the above-mentioned metabolic events except glucose and oxygen output; particularly noteworthy was its ability to augment the ‘transients’ in bile calcium and bile flow seen immediately after the administration of vasopressin with or without glucagon. T-CDCA, by contrast, produced opposite effects and attenuated all of the parameters measured, and in particular the transients in bile calcium and bile flow. The data provide evidence of a strong correlation between calcium fluxes occurring on both the sinusoidal and the bile-canalicular membranes and that all are modifiable by glucagon, Ca(2+)-mobilizing hormones and bile salts.

scholarly journals Cell swelling increases bile flow and taurocholate excretion into bile in isolated perfused rat liver

1992 ◽  
Vol 281 (3) ◽  
pp. 593-595 ◽  
Author(s):  
C Hallbrucker ◽  
F Lang ◽  
W Gerok ◽  
D Häussinger
Keyword(s):  
Bile Acid ◽  
Amino Acid ◽  
Cell Volume ◽  
Rat Liver ◽  
Bile Flow ◽  
Cell Swelling ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Volume Changes ◽  
Close Relationship

The effects of aniso-osmotically and amino-acid-induced cell-volume changes on bile flow and biliary taurocholate excretion were studied in isolated perfused rat liver. With taurocholate (100 microM) in the influent perfusate, hypo-osmotic exposure (225 mosmol/l) increased taurocholate excretion into bile and bile flow by 42 and 27% respectively, whereas inhibition by 32 and 47% respectively was observed after hyperosmotic (385 mosmol/l) exposure. The effects of aniso-moticity on taurocholate excretion into bile was observed throughout aniso-osmotic exposure, even after completion of volume-regulatory ion fluxes and were fully reversible upon re-exposure to normo-osmotic media. Hypo-osmotic cell swelling (225 mosmol/l) increased the Vmax. of taurocholate translocation from the sinusoidal compartment into bile about 2-fold. Also, cell swelling induced by glutamine and glycine stimulated both bile flow and biliary taurocholate excretion. There was a close relationship between the aniso-osmotically and amino-acid-induced change of cell volume and taurocholate excretion into bile. The data suggest that liver cell volume plays an important role in regulating bile-acid-dependent bile flow and biliary taurocholate excretion.

scholarly journals Effect of Brefeldin A on transcytotic vesicular pathway and bile secretion: A study on the isolated perfused rat liver and isolated rat hepatocyte couplets

Hepatology ◽  
1995 ◽  
Vol 21 (2) ◽  
pp. 450-459 ◽  
Author(s):  
Domenico Alvaro ◽  
Antonio Benedetti ◽  
Alessandro Gigliozzi ◽  
Adriano Bini ◽  
Sonia Furfaro ◽  
...  
Keyword(s):  
Rat Liver ◽  
Brefeldin A ◽  
Bile Secretion ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Rat Hepatocyte ◽  
Isolated Rat

Cation Transport by Slices from the Isolated Perfused Rat Liver

1972 ◽  
Vol 50 (3) ◽  
pp. 276-278
Author(s):  
A. C. Nestruck ◽  
R. W. Furneaux
Keyword(s):  
Rat Liver ◽  
Bile Flow ◽  
Cation Transport ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Bicarbonate Buffer ◽  
Potassium Efflux ◽  
Membrane Function ◽  
Surgical Preparation

Isolated livers from fed rats were perfused for 1 h with a medium consisting of Krebs–Ringer bicarbonate buffer with added albumin and glucose. Rates of perfusate and bile flow, differences in [Formula: see text], [Formula: see text], and pH, and glucose and potassium efflux were measured. Rewarmed slices of liver taken before the surgical preparation and before the perfusion were found to be able to reverse a cation shift imposed by cold incubation. Slices of liver taken after one perfusion were not able to effect this expected cation transport. It is proposed that the perfusate used was not ideal as evidenced by the altered membrane function of slices after perfusion.

Effect of Atropine on Bile Flow and Biliary Excretion of 3H-Ouabain in the Isolated Perfused Rat Liver

Pharmacology ◽  
1977 ◽  
Vol 15 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Kaisu Nevasaari ◽  
Birgitta Alakare ◽  
Niilo T. Kärki
Keyword(s):  
Rat Liver ◽  
Biliary Excretion ◽  
Bile Flow ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

scholarly journals The effects of sporidesmin and icterogenin on bile flow in the isolated perfused rat liver

1968 ◽  
Vol 110 (2) ◽  
pp. 15P-16P
Author(s):  
T F Slater ◽  
B C Sawyer ◽  
V B Delaney ◽  
G Bullock
Keyword(s):  
Rat Liver ◽  
Bile Flow ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

Endothelin-1 stimulates bile acid secretion and vesicular transport in the isolated perfused rat liver

1994 ◽  
Vol 266 (2) ◽  
pp. G324-G329 ◽  
Author(s):  
A. Tanaka ◽  
K. Katagiri ◽  
M. Hoshino ◽  
T. Hayakawa ◽  
K. Tsukada ◽  
...  
Keyword(s):  
Bile Acid ◽  
Rat Liver ◽  
Bile Flow ◽  
Low Dose ◽  
Portal Pressure ◽  
Vesicular Transport ◽  
Bile Secretion ◽  
Isolated Perfused Rat Liver ◽  
High Dose ◽  
Perfused Rat Liver

The effects of endothelin (ET) on portal pressure and bile secretion were examined using isolated perfused rat liver and rat hepatocyte preparations. ET-1 raised portal pressure dose dependently; administration at a high dose (10(-9) mol) induced a > 200% increase along with reduced bile flow and decreased secretion of bile acid and phospholipids. However, a low dose (10(-10) mol) of ET-1 brought about a < 100% portal pressure rise, enhanced both bile flow and excretion of bile acid and phospholipids, and significantly increased transfer of preadministered horseradish peroxidase (HRP) into bile. In addition, values for Ca2+ concentrations, examined by indo 1 fluorescence, were elevated in isolated hepatocytes after administration of ET-1. Papaverine suppressed the low-dose ET-1 stimulation effects on both portal pressure and bile secretion. Moreover, it also reduced the HRP excretion and suppressed intracellular Ca2+ release. This study demonstrated that ET-1 stimulates vesicular transport, probably via promotion of intracellular Ca2+ release, and, as a result, increases bile acid-dependent bile flow.

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