Lactate efflux and intracellular pH during severe hypoxia in rat cerebral cortex in vitro studied by nuclear magnetic resonance spectroscopy

1994 ◽  
Vol 178 (1) ◽  
pp. 111-114 ◽  
Author(s):  
Tiina-R.M. Pirttilä ◽  
Risto A. Kauppinen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Cerebral Cortex ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Intracellular Ph ◽  
Rat Cerebral Cortex ◽  
Resonance Spectroscopy ◽  
Cerebral Cortex In Vitro

scholarly journals A phosphorus-31 nuclear magnetic resonance investigation of intracellular environment in human normal and sickle cell blood

Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 196-209 ◽  
Author(s):  
YF Lam ◽  
AK Lin ◽  
C Ho
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Intracellular Ph ◽  
Sickle Cell ◽  
Resonance Spectroscopy ◽  
Blood Samples ◽  
Intracellular Environment ◽  
Nuclear Magnetic

Abstract Intracellular pH and 2,3-diphosphoglycerate concentration in sickle cell amenia and normal human blood samples were measured by means of phosphorus-31 nuclear magnetic resonance spectroscopy. To monitor the concentrations of various internal phosphorylated metabolites of intact red blood cells, heparinized blood samples were used and were incubated at 37 degrees C with 5.6% C92, 25% O2, and 69.4% N2. The 31P chemical shifts of phosphorylated compounds, such as 2,3-diphosphoglycerate, adenosine 5′-triphosp-ate, and inorganic phosphate, depend on pH, and by using an appropriate calibration curve, the intracellular pH of intact erythrocytes can be obtained. The intracellular pH values in fresh sickl cell blood and normal blood were found to be 7.14 and 7.29, respectively. However, the whole-blood pH, as measured by a standard pH meter, was found to be 7.54 for both types of blood. The initial concentration of 2,3-diphosphoglycerate in sickle cell blood was about 30% higher, but it was depleted much faster during incubation than that in normal blood. The difference in intracellular pH between these two types of blood samples remained constant during incubation, even after depletion of 2,3-diphosphoglycerate. These results suggest that there are differences in intracellular environment between normal and sickle cell blood. Thus, 31P nuclear magnetic resonance spectroscopy provides a fast, direct, continuous, and noninvasive way to monitor the intracellular environment of intact erythrocytes.

Cerebral intracellular pH by 31P nuclear magnetic resonance spectroscopy

Neurology ◽  
1985 ◽  
Vol 35 (6) ◽  
pp. 781-781 ◽  
Author(s):  
O. A.C. Petroff ◽  
J. W. Prichard ◽  
K. L. Behar ◽  
J. R. Alger ◽  
J. A. den Hollander ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Intracellular Ph ◽  
Resonance Spectroscopy ◽  
31P Nuclear Magnetic Resonance ◽  
Nuclear Magnetic
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