Phenolic ring deiodination in cultured rat hepatoma cells, and subcellular localization of deiodinases in cultured rat hepatoma, monkey hepatocarcinoma cells and normal rat liver homogenates

1980 ◽  
Vol 630 (4) ◽  
pp. 469-475 ◽  
Author(s):  
Kenji Sorimachi ◽  
Akira Niwa ◽  
Yasumura Yosihiro
Keyword(s):  
Subcellular Localization ◽  
Rat Liver ◽  
Hepatoma Cells ◽  
Phenolic Ring ◽  
Normal Rat ◽  
Hepatocarcinoma Cells ◽  
Liver Homogenates ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

The characterization of mitochondrial translation products in rat liver and rat hepatoma cells

FEBS Letters ◽  
1981 ◽  
Vol 126 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Jordan Kolarov ◽  
Štefan Kužela ◽  
Antek Wielburski ◽  
B.Dean Nelson
Keyword(s):  
Rat Liver ◽  
Hepatoma Cells ◽  
Mitochondrial Translation ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

scholarly journals Osmoregulated taurine transport in H4IIE hepatoma cells and perfused rat liver

1997 ◽  
Vol 321 (3) ◽  
pp. 683-690 ◽  
Author(s):  
Ulrich WARSKULAT ◽  
Matthias WETTSTEIN ◽  
Dieter HÄUSSINGER
Keyword(s):  
Rat Liver ◽  
Hepatoma Cells ◽  
Cell Swelling ◽  
Mrna Levels ◽  
Taurine Transport ◽  
H4iie Cells ◽  
Taurine Uptake ◽  
Rat Hepatoma ◽  
Taurine Efflux ◽  
Rat Hepatoma Cells

The effects of aniso-osmotic exposure on taurine transport were studied in H4IIE rat hepatoma cells. Hyperosmotic (405 mosmol/l) exposure of H4IIE cells stimulated Na+-dependent taurine uptake and led to an increase in taurine transporter (TAUT) mRNA levels, whereas hypo-osmotic (205 mosmol/l) exposure diminished both taurine uptake and TAUT mRNA levels when compared with normo-osmotic (305 mosmol/l) control incubations. Taurine uptake increased 30Ő40-fold upon raising the ambient osmolarity from 205 to 405 mosmol/l. When H4IIE cells and perfused livers were preloaded with taurine, hypo-osmotic cell swelling led to a rapid release of taurine from the cells. The taurine efflux, but not taurine uptake, was sensitive to 4,4ƀ-di-isothiocyanatostilbene-2,2ƀ-disulphonic acid (DIDS), suggestive of an involvement of DIDS-sensitive channels in mediating volume-regulatory taurine efflux. Whereas in both H4IIE rat hepatoma cells and primary hepatocytes TAUT mRNA levels were strongly dependent upon ambient osmolarity, mRNAs for other osmolyte transporters, i.e. the betaine transporter BGT-1 and the Na+/myo-inositol transporter SMIT, were not detectable. In line with this, myo-inositol uptake by H4IIE hepatoma cells was low and was not stimulated by hyperosmolarity. However, despite the absence of BGT-1 mRNA, a slight osmosensitive uptake of betaine was observed, but the rate was less than 10% of that of taurine transport. This study identifies a constitutively expressed and osmosensitive TAUT in H4IIE cells and the use of taurine as a main osmolyte, whereas betaine and myo-inositol play little or no role in the osmolyte strategy in these cells. This is in contrast with rat liver macrophages, in which betaine has been shown to be a major osmolyte.

ϵPKC Acts Like a Marker of Progressive Malignancy in Rat Liver, but Fails to Enhance Tumorigenesis in Rat Hepatoma Cells in Culture

1996 ◽  
Vol 221 (3) ◽  
pp. 688-691 ◽  
Author(s):  
Gianpaolo Perletti ◽  
Luciana Tessitore ◽  
Eliana Sesca ◽  
Paolo Pani ◽  
Mario Umberto Dianzani ◽  
...  
Keyword(s):  
Rat Liver ◽  
Hepatoma Cells ◽  
Cells In Culture ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells
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