Malate dehydrogenase isozymes in five species of sarcophagid flies (Sarcophagidae: Diptera)

Author(s):  
R.R. Tewari ◽  
Sasya Thakur
1965 ◽  
Vol 13 (6) ◽  
pp. 510-514 ◽  
Author(s):  
JAMES L. CONKLIN ◽  
EDWARD J. NEBEL

Malate dehydrogenase fractions of the chick embryo were demonstrated after starch gel electrophoresis of homogenates of liver, brain and spleen. A total of seven malate dehydrogenase fractions were observed to occur in the chick embryo in an organ specific pattern. Treatment of the homogenates with urea, sodium chloride-sodium phosphate, and p-chloromercuribenzoate prior to electrophoresis revealed that only three distinct malate dehydrogenase-active proteins were presence. Two of these proteins exhibited properties similar to those previously reported for the supernatant malate dehydrogenase and mitochondrial malate dehydrogenase of other species. Becuase of the differing properties of chick malate and lactate dehydrogenase it is concluded that the molecular basis for malate dehydrogenase isozymes is different from that reported for lactate dehydrogenase isozymes.


FEBS Letters ◽  
1973 ◽  
Vol 35 (2) ◽  
pp. 247-249 ◽  
Author(s):  
G. Dölken ◽  
D. Brdiczka ◽  
D. Pette

1971 ◽  
Vol 28 (7) ◽  
pp. 1005-1008 ◽  
Author(s):  
J. W. Clayton ◽  
D. N. Tretiak ◽  
A. H. Kooyman

Electrophoresis of extracts of white skeletal muscle revealed a total of six phenotypes of malate dehydrogenase (MDH) (L-malate; NAD oxidoreductase, EC 1.1.1.37) isozymes in walleye (Stizostedion vitreum vitreum). The individual MDH phenotypes consisted of four or more MDH isozymes.The heritability of all six phenotypes was tested in a breeding experiment. The results of the breeding experiment provided evidence for the existence of three nondominant alleles at one MDH locus. Subcellular fractionations indicated that these three alleles code for a supernatant (extramitochondrial) form of MDH. The frequencies of these alleles varied in fish from four locations in western Canada.


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