In vitro and in vivo modulation of 5-hydroxytryptamine-, thyrotropin-releasing hormone- and calcitonin-gene related peptide-like immunoreactivities in adult rat sensory neurons

Neuroscience ◽  
1992 ◽  
Vol 51 (2) ◽  
pp. 401-410 ◽  
Author(s):  
P. Delree ◽  
D. Martin ◽  
C. Sadzot-Delvaux ◽  
B. Rogister ◽  
P. Leprince ◽  
...  
Keyword(s):  
Sensory Neurons ◽  
Calcitonin Gene Related Peptide ◽  
Thyrotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Adult Rat ◽  
Related Peptide ◽  
Calcitonin Gene

scholarly journals Evidence that α-Calcitonin Gene-Related Peptide Is a Neurohormone that Controls Systemic Lipid Availability and Utilization

Endocrinology ◽  
2007 ◽  
Vol 149 (1) ◽  
pp. 154-160 ◽  
Author(s):  
Rachel N. Danaher ◽  
Kerry M. Loomes ◽  
Bridget L. Leonard ◽  
Lynda Whiting ◽  
Debbie L. Hay ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Physiological Role ◽  
Calcitonin Gene Related Peptide ◽  
Physiological Regulation ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Motor Nerves ◽  
In Vivo Effects

α-Calcitonin gene-related peptide (αCGRP) is released mainly from sensory and motor nerves in response to physiological stimuli. Despite well-documented pharmacological effects, its primary physiological role has thus far remained obscure. Increased lipid content, particularly in skeletal muscle and liver, is strongly implicated in the pathogenesis of insulin resistance, but the physiological regulation of organ lipid is imperfectly understood. Here we report our systematic investigations of the effects of αCGRP on in vitro and in vivo indices of lipid metabolism. In rodents, levels of αCGRP similar to those in the blood markedly stimulated fatty acid β-oxidation and evoked concomitant mobilization of muscle lipid via receptor-mediated activation of muscle lipolysis. αCGRP exerted potent in vivo effects on lipid metabolism in muscle, liver, and the blood via receptor-mediated pathways. Studies with receptor antagonists were consistent with tonic regulation of lipid metabolism by an endogenous CGRP agonist. These data reveal that αCGRP is a newly recognized regulator of lipid availability and utilization in key tissues and that it may elevate the availability of intramyocellular free fatty acids to meet muscle energy requirements generated by contraction by evoking their release from endogenous triglyceride.

Comparison of the in vitro and in vivo pharmacology of adrenomedullin, calcitonin gene-related peptide and amylin in rats

1996 ◽  
Vol 314 (1-2) ◽  
pp. 115-121 ◽  
Author(s):  
William Vine ◽  
Kevin Beaumont ◽  
Bronislava Gedulin ◽  
Richard Pittner ◽  
Candace X. Moore ◽  
...  
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

scholarly journals Evaluation of calcitonin gene-related peptide prolonged release tablet in vitro and in vivo.

1996 ◽  
Vol 11 (1) ◽  
pp. 49-54 ◽  
Author(s):  
Toshihiko Kaminuma ◽  
Nobuhiko Ochiai ◽  
Ritsuko Ehama ◽  
Masahiro Tajima ◽  
Kazuo Watabe ◽  
...  
Keyword(s):  
Calcitonin Gene Related Peptide ◽  
Prolonged Release ◽  
Related Peptide ◽  
Calcitonin Gene ◽  
Release Tablet

Gene transfer of calcitonin gene-related peptide to cerebral arteries

2000 ◽  
Vol 278 (2) ◽  
pp. H586-H594 ◽  
Author(s):  
Kazunori Toyoda ◽  
Frank M. Faraci ◽  
Andrew F. Russo ◽  
Beverly L. Davidson ◽  
Donald D. Heistad
Keyword(s):  
Cerebrospinal Fluid ◽  
Gene Transfer ◽  
Basilar Artery ◽  
Cultured Cells ◽  
Cerebral Arteries ◽  
Calcitonin Gene Related Peptide ◽  
Related Peptide ◽  
Calcitonin Gene

Overexpression of calcitonin gene-related peptide (CGRP), an extremely potent vasodilator, to blood vessels is a possible strategy for prevention of vasospasm. We constructed an adenoviral vector that encodes prepro-CGRP (Adprepro-CGRP) and examined the effects of gene transfer on cultured cells and cerebral arteries. Transfection of Adprepro-CGRP to Cos-7 and NIH-3T3 cells increased CGRP-like immunoreactivity in media and produced an increase in cAMP in recipient cells. Five days after injection of Adprepro-CGRP into the cisterna magna of rabbits, the concentration of CGRP-like immunoreactivity increased by 93-fold in cerebrospinal fluid. In basilar artery, cAMP increased by 2.3-fold after Adprepro-CGRP compared with a control adenovirus. After transfection of Adprepro-CGRP, contraction of basilar artery in vitro to histamine and serotonin was attenuated, and relaxation to an inhibitor of cyclic nucleotide phosphodiesterase 3-isobutyl-1-methylxanthine was augmented compared with nontransduced arteries or arteries transfected with a control gene. Altered vascular responses were restored to normal by pretreatment with a CGRP1 receptor antagonist CGRP-(8–37). Thus gene transfer of prepro-CGRP in vivo overexpresses CGRP in cerebrospinal fluid and perivascular tissues and modulates vascular tone. We speculate that this approach may be useful in prevention of vasospasm after subarachnoid hemorrhage.

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