Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli

Gene ◽

10.1016/0378-1119(85)90003-4 ◽

1985 ◽

Vol 35 (3) ◽

pp. 249-258 ◽

Author(s):

Michael L. Kotewicz ◽

James M. D'Alessio ◽

Katharine M. Driftmier ◽

Karen P. Blodgett ◽

Gary F. Gerard

Keyword(s):

Escherichia Coli ◽

Reverse Transcriptase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Murine Leukemia

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Defects in Moloney murine leukemia virus replication caused by a reverse transcriptase mutation modeled on the structure of Escherichia coli RNase H.

Journal of Virology ◽

10.1128/jvi.66.2.615-622.1992 ◽

1992 ◽

Vol 66 (2) ◽

pp. 615-622 ◽

Author(s):

A Telesnitsky ◽

S W Blain ◽

S P Goff

Keyword(s):

Escherichia Coli ◽

Reverse Transcriptase ◽

Virus Replication ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Rnase H ◽

Moloney Murine Leukemia Virus ◽

Murine Leukemia

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Escherichia coli DNA polymerase III ε subunit increases Moloney murine leukemia virus reverse transcriptase fidelity and accuracy of RT–PCR procedures

Analytical Biochemistry ◽

10.1016/j.ab.2006.10.009 ◽

2007 ◽

Vol 360 (1) ◽

pp. 84-91 ◽

Author(s):

Bahram Arezi ◽

Holly H. Hogrefe

Keyword(s):

Escherichia Coli ◽

Reverse Transcriptase ◽

Dna Polymerase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Dna Polymerase Iii ◽

Rt Pcr ◽

Polymerase Iii ◽

Murine Leukemia

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Expression in Escherichia coli of a Moloney murine leukemia virus reverse transcriptase whose structure closely resembles the viral enzyme

Gene ◽

10.1016/0378-1119(88)90369-1 ◽

1988 ◽

Vol 66 (2) ◽

pp. 319-323 ◽

Author(s):

Amnon Hizi ◽

Stephen H. Hughes

Keyword(s):

Escherichia Coli ◽

Reverse Transcriptase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Expression In Escherichia Coli ◽

Viral Enzyme ◽

Murine Leukemia

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A novel and simple method for high-level production of reverse transcriptase from Moloney murine leukemia virus (MMLV-RT) in Escherichia coli

Biotechnology Letters ◽

10.1007/s10529-009-9977-5 ◽

2009 ◽

Vol 31 (7) ◽

pp. 1051-1057 ◽

Author(s):

Yu Chen ◽

Weiguo Xu ◽

Qiming Sun

Keyword(s):

Escherichia Coli ◽

Reverse Transcriptase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Simple Method ◽

Level Production ◽

Murine Leukemia

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Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errors

The EMBO Journal ◽

10.1093/emboj/16.4.856 ◽

1997 ◽

Vol 16 (4) ◽

pp. 856-865 ◽

Author(s):

D. Kulpa

Keyword(s):

Reverse Transcriptase ◽

Reverse Transcription ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Strand Transfer ◽

Murine Leukemia

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Soluble Expression and Purification of Histidine-Tagged Moloney Murine Leukemia Virus Reverse Transcriptase by Ni-NTA Affinity Chromatography

Affinity Chromatography ◽

10.5772/34778 ◽

2012 ◽

Author(s):

Xie Fei ◽

Ma Xuemei ◽

Wang Xiansong

Keyword(s):

Reverse Transcriptase ◽

Affinity Chromatography ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Soluble Expression ◽

Moloney Murine Leukemia Virus ◽

Expression And Purification ◽

Murine Leukemia

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Crystal structures of an N-terminal fragment from moloney murine leukemia virus reverse transcriptase complexed with nucleic acid: functional implications for template-primer binding to the fingers domain 1 1Edited by D. C. Rees

Journal of Molecular Biology ◽

10.1006/jmbi.1999.3477 ◽

2000 ◽

Vol 296 (2) ◽

pp. 613-632 ◽

Author(s):

Shabir Najmudin ◽

Marie L. Coté ◽

Dunming Sun ◽

Sarah Yohannan ◽

Sherwin P. Montano ◽

...

Keyword(s):

Nucleic Acid ◽

Reverse Transcriptase ◽

Crystal Structures ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Moloney Murine Leukemia Virus ◽

Terminal Fragment ◽

Functional Implications ◽

Murine Leukemia

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Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase

Protein Expression and Purification ◽

10.1016/j.pep.2015.04.012 ◽

2015 ◽

Vol 113 ◽

pp. 44-50 ◽

Author(s):

Kosaku Nishimura ◽

Kanta Yokokawa ◽

Tetsuro Hisayoshi ◽

Kosuke Fukatsu ◽

Ikumi Kuze ◽

...

Keyword(s):

Reverse Transcriptase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Rnase H ◽

Moloney Murine Leukemia Virus ◽

Murine Leukemia

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Amino acid substitutions away from the RNase H catalytic site increase the thermal stability of Moloney murine leukemia virus reverse transcriptase through RNase H inactivation

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2014.10.044 ◽

2014 ◽

Vol 454 (2) ◽

pp. 269-274 ◽

Author(s):

Atsushi Konishi ◽

Tetsuro Hisayoshi ◽

Kanta Yokokawa ◽

Verónica Barrioluengo ◽

Luis Menéndez-Arias ◽

...

Keyword(s):

Thermal Stability ◽

Reverse Transcriptase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Rnase H ◽

Moloney Murine Leukemia Virus ◽

Amino Acid Substitutions ◽

Thermal Stability Of ◽

Murine Leukemia

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RNase H domain mutations affect the interaction between Moloney murine leukemia virus reverse transcriptase and its primer-template.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.90.4.1276 ◽

1993 ◽

Vol 90 (4) ◽

pp. 1276-1280 ◽

Author(s):

A. Telesnitsky ◽

S. P. Goff

Keyword(s):

Reverse Transcriptase ◽

Murine Leukemia Virus ◽

Leukemia Virus ◽

Rnase H ◽

Moloney Murine Leukemia Virus ◽

Murine Leukemia

Download Full-text