The RNA polymerase I initiation site and the external transcribed spacer of the fission yeast Schizosaccharomyces pombe ribosomal RNA genes

Gene ◽  
1985 ◽  
Vol 39 (2-3) ◽  
pp. 165-172 ◽  
Author(s):  
Elisabetta Balzi ◽  
Attilio Di Pietro ◽  
André Goffeau ◽  
Harm van Heerikhuizen ◽  
Jacobus Klootwijk
Keyword(s):  
Rna Polymerase ◽  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Ribosomal Rna ◽  
Initiation Site ◽  
Rna Polymerase I ◽  
Ribosomal Rna Genes ◽  
External Transcribed Spacer ◽  
Polymerase I ◽  
Rna Genes

scholarly journals Interaction of nucleolin with ribosomal RNA genes and its role in RNA polymerase I transcription

2012 ◽  
Vol 40 (19) ◽  
pp. 9441-9454 ◽  
Author(s):  
Rong Cong ◽  
Sadhan Das ◽  
Iva Ugrinova ◽  
Sanjeev Kumar ◽  
Fabien Mongelard ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Rna Polymerase I ◽  
Ribosomal Rna Genes ◽  
Polymerase I ◽  
Rna Genes

Complex organization of the length heterogeneous 5′ external spacer of mung bean (Vigna radiata) ribosomal DNA

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 723-733 ◽  
Author(s):  
Jutta Gerstner ◽  
Katrin Schiebel ◽  
Georg von Waldburg ◽  
Vera Hemleben
Keyword(s):  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Ribosomal Dna ◽  
Vigna Radiata ◽  
Mung Bean ◽  
Rna Polymerase I ◽  
Ribosomal Rna Genes ◽  
Enzyme Analysis ◽  
Polymerase I ◽  
Rna Genes

Restriction enzyme analysis and cloning of the 18S, 5.8S, and 25S ribosomal RNA genes (rDNA) of the mung bean (Vigna radiata = Phaseolus aureus) reveal length heterogeneity in the repeating units (10 – 11 kbp) localized within two different regions in the ribosomal spacer. The 1.5 – 2.0 kbp region flanking the 3′ end of the 25S rRNA contains various numbers (8 – 10) of tandemly arranged 180 bp subrepeats. After DNA sequencing a complex organized length heterogeneous 5′ external spacer built up of different numbers of 340 bp subrepeats, each flanked by 52 bp direct repeats, is detected and described for the first time for plant ribosomal DNA repeating units. Sequences occurring in front of and within this repeated structure (elements II – IV) can be combined with the motifs P1, P2, and P3. These exhibit a strong similarity to transcription initiation sites specific for RNA polymerase I described for other plant and animal rDNA investigated to date. Transcription products complementary to the complex repeated structures are detected by hybridization to total nuclear RNA. The 9 bp element V located in front of the first 340 bp region appears in duplicated form as a direct repeat with sequence similarity to SV40 (or RNA polymerase II) enhancer sequences.Key words: promoter, ribosomal RNA genes, RNA polymerase I, spacer organization, transcription.

scholarly journals A unique enhancer boundary complex on the mouse ribosomal RNA genes persists after loss of Rrn3 or UBF and the inactivation of RNA polymerase I transcription

PLoS Genetics ◽  
2017 ◽  
Vol 13 (7) ◽  
pp. e1006899 ◽  
Author(s):  
Chelsea Herdman ◽  
Jean-Clement Mars ◽  
Victor Y. Stefanovsky ◽  
Michel G. Tremblay ◽  
Marianne Sabourin-Felix ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Ribosomal Rna ◽  
Rna Polymerase I ◽  
Ribosomal Rna Genes ◽  
Boundary Complex ◽  
Polymerase I ◽  
Rna Genes

scholarly journals RNA polymerase I transcription fidelity, speed and processivity depend on the interplay of its lobe binding subunits

2018 ◽  
Author(s):  
Philipp E. Merkl ◽  
Michael Pilsl ◽  
Tobias Fremter ◽  
Gernot Längst ◽  
Philipp Milkereit ◽  
...  
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Ribosomal Rna ◽  
Rna Polymerase I ◽  
Pol Ii ◽  
Transcription System ◽  
Pol I ◽  
Polymerase I ◽  
Rna Genes

AbstractEukaryotic RNA polymerases I and III (Pol I and III) consist of core subunits, which are conserved in RNA polymerase II (Pol II). Additionally, Pol I and III have specific subunits, associating with the so-called ‘lobe’ structure first described within Pol II. In Pol I of the yeast S. cerevisiae, these are Rpa34.5, and the N-terminal domains of Rpa49 and Rpa12.2, here referred to as the lobe-binding module (lb-module). We analyzed functions of the lb-module in a defined in vitro transcription system. Cooperation between lb-module components influenced transcription fidelity, elongation speed, and release of stalled Pol I complexes to continue elongation. Interestingly, lb-module containing Pol I and III, but not Pol II, were able to transcribe nucleosomal templates. Our data suggest, how the Pol I specific subunits may contribute to accurate and processive transcription of ribosomal RNA genes.

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