Screening with a shell vial assay for antiviral activity against cytomegalovirus

1989 ◽  
Vol 12 (1) ◽  
pp. 5-8 ◽  
Author(s):  
A. Telenti ◽  
T.F. Smith
Keyword(s):  
Antiviral Activity ◽  
Shell Vial ◽  
Shell Vial Assay

scholarly journals Bacteriostatic and Bactericidal Activities of Tigecycline against Coxiella burnetii and Comparison with Those of Six Other Antibiotics

2009 ◽  
Vol 53 (6) ◽  
pp. 2690-2692 ◽  
Author(s):  
Ioanna Spyridaki ◽  
Anna Psaroulaki ◽  
Iosif Vranakis ◽  
Yannis Tselentis ◽  
Achilleas Gikas
Keyword(s):  
Coxiella Burnetii ◽  
Q Fever ◽  
Vero Cells ◽  
In Vitro Susceptibility ◽  
Shell Vial ◽  
Bactericidal Activities ◽  
Acute Q Fever ◽  
Reference Strains ◽  
Shell Vial Assay

ABSTRACT The present article is a study of the in vitro susceptibility of eight Greek Coxiella burnetii isolates, derived from patients with acute Q fever, and two reference strains of Coxiella burnetii to tigecycline. The bacteriostatic activity of tigecycline was compared with those of six other antibiotics using a shell vial assay. The MICs of the examined antibiotics were as follows: tigecycline ranged from 0.25 to 0.5 μg/ml; doxycycline, trovafloxacin, and ofloxacin ranged from 1 to 2 μg/ml; linezolid and clarithromycin ranged from 2 to 4 μg/ml; and ciprofloxacin ranged from 4 to 8 μg/ml. Tigecycline was effective in inhibiting the infection of Vero cells by C. burnetii. No bactericidal activity was observed against C. burnetii at 4 μg/ml.

scholarly journals Relevance of Influenza A Virus Detection by PCR, Shell Vial Assay, and Tube Cell Culture to Rapid Reporting Procedures

2006 ◽  
Vol 44 (9) ◽  
pp. 3366-3367 ◽  
Author(s):  
N. L. Zitterkopf ◽  
S. Leekha ◽  
M. J. Espy ◽  
C. M. Wood ◽  
P. Sampathkumar ◽  
...  
Keyword(s):  
Cell Culture ◽  
Influenza A Virus ◽  
Influenza A ◽  
Virus Detection ◽  
Shell Vial ◽  
Tube Cell ◽  
Reporting Procedures ◽  
Shell Vial Assay

scholarly journals Multisite evaluation of a monoclonal antibody reagent (Syva) for rapid diagnosis of cytomegalovirus in the shell vial assay.

1989 ◽  
Vol 27 (7) ◽  
pp. 1502-1505 ◽  
Author(s):  
D J Jespersen ◽  
W L Drew ◽  
C A Gleaves ◽  
J D Meyers ◽  
A L Warford ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rapid Diagnosis ◽  
Shell Vial ◽  
Shell Vial Assay

scholarly journals Use of Molecular Assays in Diagnosis and Monitoring of Cytomegalovirus Disease following Renal Transplantation

1999 ◽  
Vol 37 (9) ◽  
pp. 2804-2807 ◽  
Author(s):  
Celia Aitken ◽  
Winsome Barrett-Muir ◽  
Colin Millar ◽  
Kate Templeton ◽  
Janice Thomas ◽  
...  
Keyword(s):  
Viral Load ◽  
Cytomegalovirus Disease ◽  
Pcr Assay ◽  
Shell Vial ◽  
Viral Loads ◽  
Molecular Assays ◽  
Transplant Patients ◽  
Cmv Disease ◽  
Shell Vial Assay

We compared two commercial molecular assays (the Murex Hybrid Capture CMV DNA assay [HCA], version 2, and the Roche Amplicor plasma PCR assay) with a standard shell vial assay in detecting and predicting cytomegalovirus (CMV) disease in a group of renal transplant patients and assessed the role of viral load measurements (using the HCA) in their management. The sensitivity of the HCA and Amplicor assay in terms of disease detection was 100%, compared to 71% for the shell vial assay. Both the HCA and the PCR assay detected all cases of disease, at medians of 11 and 12.5 days before the onset of symptoms, respectively. Significantly higher viral loads were detected in those patients with symptoms (7.9 × 105 copies/ml) than in patients without symptoms (7.9 × 104 copies/ml;P < 0.0001). There was also a trend towards higher viral loads in those patients with primary infections (7.8 × 105 copies/ml) than in those patients with reactivations of CMV disease or reinfections. Successful treatment with ganciclovir was associated with a >90% reduction in viral load. Both of these new assays are sensitive and easy to use. A comparison of accurate quantitation is also useful in monitoring responses to antiviral therapy.

scholarly journals Rapid Detection of Respiratory Viruses by Shell Vial Assay Using Simultaneous Culture of HEp-2, LLC-MK2, and MDCK Cells in a Single Vial

1999 ◽  
Vol 37 (10) ◽  
pp. 3436-3436
Author(s):  
José M. Navarro-Marí ◽  
Sara Sanbonmatsu-Gámez ◽  
Mercedes Pérez-Ruiz ◽  
Manuel De La Rosa-Fraile
Keyword(s):  
Rapid Detection ◽  
Mdck Cells ◽  
Respiratory Viruses ◽  
Shell Vial ◽  
Shell Vial Assay

scholarly journals In Vitro Susceptibility of Coxiella burnetii to Trovafloxacin in Comparison with Susceptibilities to Pefloxacin, Ciprofloxacin, Ofloxacin, Doxycycline, and Clarithromycin

1998 ◽  
Vol 42 (10) ◽  
pp. 2747-2748 ◽  
Author(s):  
Achilleas Gikas ◽  
Ioanna Spyridaki ◽  
Anna Psaroulaki ◽  
Diamantis Kofterithis ◽  
Yannis Tselentis
Keyword(s):  
Coxiella Burnetii ◽  
In Vitro Susceptibility ◽  
Shell Vial ◽  
Antibiotic Susceptibilities ◽  
Shell Vial Assay

ABSTRACT The antibiotic susceptibilities of eight Greek isolates ofCoxiella burnetii to trovafloxacin were determined by the shell vial assay. MICs of trovafloxacin and ofloxacin ranged from 1 to 2 μg/ml, those of pefloxacin ranged from 1 to 4 μg/ml, those of ciprofloxacin ranged from 4 to 8 μg/ml, those of doxycycline ranged from 1 to 2 μg/ml, and those of clarithromycin ranged from 2 to 4 μg/ml. Trovafloxacin exhibited no activity against C. burnetii at 4 μg/ml.

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