Isolation and biochemical characterization of the microsomal fraction from the digestive gland of mussel Mytilus galloprovincialis Lam.

Cultures of the mussel Mytilus galloprovincialis are frequently affected by accumulation of the amnesic shellfish poisoning toxin domoic acid (DA). This species is characterized by a fast uptake and release of the toxin. In this work, the main characteristics of the uptake mechanism have been studied by incubation of digestive gland thin slices in media with different composition and DA concentration. DA uptake seems to follow Michaelis–Menten kinetics, with a very high estimated KM (1722 µg DA mL−1) and a Vmax of 71.9 µg DA g−1 h−1, which is similar to those found for other amino acids in invertebrates. Replacement of NaCl from the incubation media by Cl-choline (Na+-free medium) did not significantly reduce the uptake, but replacement by sorbitol (Na+-free and Cl−-depleted medium) did. A new experiment replacing all chlorides with their equivalent gluconates (Na+- and Cl−-free medium) showed an important reduction in the uptake that should be attributed to the absence of chloride, pointing to a Na+-independent, Cl− (or anion-) dependent transporter. In media with Na+ and Cl−, neither decreasing the pH nor adding cyanide (a metabolic inhibitor) had significant effect on DA uptake, suggesting that the transport mechanism is not H+- or ATP-dependent. In a chloride depleted medium, lowering pH or adding CN increased the uptake, suggesting that other anions could, at least partially, substitute chloride.

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Biochemical characterization of a copper-thionein involved in Cu accumulation in the lysosomes of the digestive gland of mussels exposed to the metal

Marine Environmental Research ◽

10.1016/0141-1136(88)90286-3 ◽

1988 ◽

Vol 24 (1-4) ◽

pp. 163-166 ◽

Cited By ~ 11

Author(s):

A. Viarengo ◽

L. Canesi ◽

M. Pertica ◽

G. Mancinelli ◽

M. Orunesu ◽

...

Keyword(s):

Digestive Gland ◽

Biochemical Characterization ◽

Cu Accumulation

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Biochemical characterization of the native α-carbonic anhydrase purified from the mantle of the Mediterranean mussel, Mytilus galloprovincialis

Journal of Enzyme Inhibition and Medicinal Chemistry ◽

10.1080/14756366.2017.1284069 ◽

2017 ◽

Vol 32 (1) ◽

pp. 632-639 ◽

Cited By ~ 18

Author(s):

Rosa Perfetto ◽

Sonia Del Prete ◽

Daniela Vullo ◽

Giovanni Sansone ◽

Carmela Barone ◽

...

Keyword(s):

Carbonic Anhydrase ◽

Mytilus Galloprovincialis ◽

Biochemical Characterization ◽

Mediterranean Mussel ◽

The Mediterranean

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Purification and biochemical characterization of a lysosomal copper-rich thionein-like protein involved in metal detoxification in the digestive gland of mussels

Comparative Biochemistry and Physiology Part C Comparative Pharmacology ◽

10.1016/0742-8413(89)90252-1 ◽

1989 ◽

Vol 93 (2) ◽

pp. 389-395 ◽

Cited By ~ 9

Author(s):

A. Viarengo ◽

M. Pertica ◽

L. Canesi ◽

A. Mazzucotelli ◽

M. Orunesu ◽

...

Keyword(s):

Digestive Gland ◽

Biochemical Characterization ◽

Metal Detoxification

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Purification and biochemical characterization of a cadmium metallothionein from the digestive gland of the Antarctic scallop Adamussium colbecki (Smith, 1902)

Polar Biology ◽

10.1007/s003000000186 ◽

2001 ◽

Vol 24 (3) ◽

pp. 147-153 ◽

Cited By ~ 12

Author(s):

E. Ponzano ◽

F. Dondero ◽

J.-M. Bouquegneau ◽

R. Sack ◽

P. Hunziker ◽

...

Keyword(s):

Digestive Gland ◽

Biochemical Characterization ◽

The Antarctic ◽

Adamussium Colbecki

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Biochemical characterization of the plasma membrane Ca2+ pumping ATPase activity present in the gill cells of Mytilus galloprovincialis LAM

Comparative Biochemistry and Physiology Part B Comparative Biochemistry ◽

10.1016/0305-0491(91)90285-l ◽

1991 ◽

Vol 100 (4) ◽

pp. 753-758 ◽

Cited By ~ 3

Author(s):

A. Viarengo ◽

M. Pertica ◽

G. Mancinelli ◽

G. Damonte ◽

M. Orunesu

Keyword(s):

Plasma Membrane ◽

Atpase Activity ◽

Mytilus Galloprovincialis ◽

Biochemical Characterization ◽

Gill Cells

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Plant Pathology Diagnosed by X-Ray Microanalysis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100129152 ◽

1987 ◽

Vol 45 ◽

pp. 974-975

Author(s):

J. H. Resau ◽

N. Howell ◽

S. H. Chang

Keyword(s):

Electron Microscopy ◽

Plant Pathology ◽

Biochemical Characterization ◽

Nutrient Deficiency ◽

Green Leaf ◽

Parasitic Infestation ◽

X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

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