A set of inter-Alu PCR markers for chromosome 21 generated from pulsed-field gel-fractionated NotI restriction fragments

Genomics ◽  
1995 ◽  
Vol 26 (2) ◽  
pp. 318-326 ◽  
Author(s):  
Denan Wang ◽  
Yiwen Zhu ◽  
Cassandra L. Smith
Keyword(s):  
Chromosome 21 ◽  
Pcr Markers ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Noti Restriction

scholarly journals Assignment of ten DNA repair genes from Schizosaccharomyces pombe to chromosomal NotI restriction fragments

1991 ◽  
Vol 228 (3) ◽  
pp. 470-472 ◽  
Author(s):  
Bernard C. Broughton ◽  
Nik Barbet ◽  
Johanne Murray ◽  
Felicity Z. Watts ◽  
Marcel H. M. Koken ◽  
...  
Keyword(s):  
Dna Repair ◽  
Schizosaccharomyces Pombe ◽  
Dna Repair Genes ◽  
Restriction Fragments ◽  
Repair Genes ◽  
Noti Restriction

scholarly journals Use of pulsed field gel electrophoresis and transposon mutagenesis to estimate the minimal number of genes required for motility in Caulobacter crescentus.

Genetics ◽  
1989 ◽  
Vol 123 (4) ◽  
pp. 649-654 ◽  
Author(s):  
B Ely ◽  
T W Ely
Keyword(s):  
Gel Electrophoresis ◽  
Caulobacter Crescentus ◽  
Pulsed Field Gel Electrophoresis ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Transposon Insertion ◽  
Number Of Genes ◽  
Physical And Genetic Map ◽  
Insertion Mutations ◽  
Flagellar Genes

Abstract To facilitate the mapping of transposon insertion mutations in Caulobacter crescentus, we have used pulsed field gel electrophoresis to construct a detailed physical and genetic map of the C. crescentus genome. Restriction fragments were generated by DraI, AseI, or SpeI which cleave the C. crescentus 40, 13, and 26 times, respectively, and Tn5 insertions were used to align the restriction fragments generated by each of the enzymes. The utility of the resulting map was demonstrated by determining the chromosomal locations of a collection of flagellar mutations. As a result of this study, we were able to identify ten new flagellar genes at various locations on the chromosome. Thus, at least 48 genes are required for the assembly of a functional flagellum in C. crescentus.

scholarly journals Evaluation of pulsed-field gel electrophoresis of genomic restriction fragments in the discrimination of Yersinia enterocolitica O[ratio ]3

1998 ◽  
Vol 121 (3) ◽  
pp. 579-586 ◽  
Author(s):  
K. ASPLUND ◽  
T. JOHANSSON ◽  
A. SIITONEN
Keyword(s):  
Yersinia Enterocolitica ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Restriction Fragments ◽  
Pulsed Field ◽  
Different Types

One hundred and six Yersinia enterocolitica serogroup O[ratio ]3, biotype 4 isolated from human and porcine samples in 1984 and in the years 1993–5 were examined by pulsed-field gel electrophoresis (PFGE). The genomic profiles produced by the enzymes NotI and XbaI were studied. Sixteen (A–P) and 8 (1·8) different pulsotypes were obtained, respectively. By combining the pulsotypes produced by both NotI and XbaI 24 different types were distinguished. The two major types, designated as A1 and B1, comprised 36% of all strains tested. The proportions of pulsotypes A1 and B1 were, 35·9 and 25·6%, respectively, among strains isolated in 1984. The corresponding figures among the strains isolated in 1993–5 were 35·8 and 41·8%. Nine pulsotypes were found only in 1984 and nine only in 1993–5. The proportions of the major pulsotypes, A1 and B1, in human isolates were 42·9 and 35·7% and in porcine isolates 22·2 and 36·1% respectively. Six types were found among both human and porcine isolates, 8 only among human strains and 10 only among porcine strains.

scholarly journals Pulsed-field gel electrophoresis of the genomic restriction fragments of coagulase-negative staphylococci

1994 ◽  
Vol 124 (2) ◽  
pp. 131-139 ◽  
Author(s):  
ŠáRka Snopková ◽  
Friedrich Götz ◽  
Jiří DoÅ¡kaÅ™ ◽  
Stanislav Rosypal
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Coagulase Negative Staphylococci ◽  
Restriction Fragments ◽  
Pulsed Field

A Linkage Map of Human Chromosome 21: 43 PCR Markers at Average Intervals of 2.5 cM

Genomics ◽  
1993 ◽  
Vol 16 (3) ◽  
pp. 562-571 ◽  
Author(s):  
Melvin G. McInnis ◽  
Aravinda Chakravarti ◽  
Jillian Blaschak ◽  
Michael B. Petersen ◽  
Vikram Sharma ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Linkage Map ◽  
Chromosome 21 ◽  
Pcr Markers ◽  
Human Chromosome 21
Sign in / Sign up

Export Citation Format

Share Document