Induction and rejoining of radiation-induced DNA single-strand breaks: “tail moment” as a function of position in the cell cycle

1993 ◽  
Vol 294 (3) ◽  
pp. 275-283 ◽  
Author(s):  
Peggy L. Olive ◽  
Judit P. Banánth
Keyword(s):  
Cell Cycle ◽  
Single Strand ◽  
Tail Moment ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Radiation Induced

scholarly journals SisterC: A novel 3C-technique to detect chromatin interactions between and along sister chromatids

2020 ◽  
Author(s):  
Marlies E. Oomen ◽  
Adam K. Hedger ◽  
Jonathan K. Watts ◽  
Job Dekker
Keyword(s):  
Cell Cycle ◽  
Single Strand ◽  
Chromosome Conformation Capture ◽  
Brdu Incorporation ◽  
Chromosome Conformation ◽  
Strand Breaks ◽  
Chromatin Interactions ◽  
Sister Chromatids ◽  
Single Strand Breaks ◽  
Capture Techniques

Abstract Current chromosome conformation capture techniques are not able to distinguish sister chromatids. Here we describe the protocol of SisterC1: a novel Hi-C technique that leverages BrdU incorporation and UV/Hoechst-induced single strand breaks to identify interactions along and between sister chromatids. By synchronizing cells, BrdU is incorporated only on the newly replicated strand, which distinguishes the two sister chromatids2,3. This is followed by Hi-C4 of cells that can be arrested in different stages of the cell cycle, e.g. in mitosis. Before final amplification of the Hi-C library, strands containing BrdU are specifically depleted by UV/Hoechst treatment. SisterC libraries are then sequenced using 50bp paired end reads, followed by mapping using standard Hi-C processing tools. Interactions can then be assigned as inter- or intra-sister interactions based on read orientation.

Chemical Nature of Radiation-Induced Single-Strand Breaks in the DNA of Dormant Barley Seeds in Vivo

1973 ◽  
Vol 55 (2) ◽  
pp. 324 ◽  
Author(s):  
Ahmed El-Metainy ◽  
Shigemitsu Tano ◽  
Keiji Yano ◽  
Hikoyuki Yamaguchi
Keyword(s):  
Chemical Nature ◽  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Radiation Induced

Radiation-induced single-strand breaks in the DNA of dormant barley seeds

1971 ◽  
Vol 13 (4) ◽  
pp. 337-344 ◽  
Author(s):  
Ahmed El-Metainy ◽  
Masamichi Takagi ◽  
Shigemetsu Tano ◽  
Hikoyuki Yamaguchi
Keyword(s):  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Radiation Induced
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