Hirsutoid papillomas of vulvae, absence of human papilloma virus (HPV) DNA by the polymerase chain reaction

1993 ◽  
Vol 6 (1) ◽  
pp. 87
Author(s):  
Wen-Yuan Zhu ◽  
Ming-Yu Xia ◽  
Hong-Xia Li ◽  
Wei Wang ◽  
Zan Xu
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Polymerase Chain

scholarly journals Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction.

1988 ◽  
Vol 167 (1) ◽  
pp. 225-230 ◽  
Author(s):  
D K Shibata ◽  
N Arnheim ◽  
W J Martin
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Dna Sequences ◽  
Polymerase Chain Reaction Amplification ◽  
Cdna Probe ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Hpv Dna ◽  
Polymerase Chain

Human papilloma virus (HPV) DNA sequences have been detected in paraffin-embedded tissue using an enzymatic in vitro amplification technique known as the polymerase chain reaction. Amplification of a HPV DNA sequence before its detection with a cDNA probe significantly increases the rapidity as well as the sensitivity of detection such that a single 5-10-micron thick paraffin-embedded tissue section can be analyzed within 24 h. The assay specifically detected HPV 16 or 18 without crossreactivity with HPV 6 or 11. As few as 20 viral copies could be detected. The rapid and sensitive analysis of HPV in normal and pathological tissues using this technique may contribute significantly to identifying the role of HPV as a risk factor in carcinoma.

Human papilloma virus detection in liquid cytology, in situ hybridization and polymerase chain reaction

2005 ◽  
Vol 446 (2) ◽  
pp. 202-203 ◽  
Author(s):  
F. Alameda ◽  
L. Pijuan ◽  
L. Ferrer ◽  
M. L. Mari�oso ◽  
M. Muset ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Human Papilloma Virus ◽  
Virus Detection ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain

Uptake of exogenous human papilloma virus L1 DNA by oocytes and detection by the polymerase chain reaction

1992 ◽  
Vol 9 (6) ◽  
pp. 531-533 ◽  
Author(s):  
Philip J. Chan ◽  
Brian C. Su ◽  
Donald R. Tredway ◽  
Majid Seraj ◽  
Ibrahim M. Seraj ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Polymerase Chain

scholarly journals Presencia de lesiones preinvasoras e invasoras de cérvix, relación con el virus papiloma humano y factores epidemiológicos en Mérida, Venezuela

2018 ◽  
Vol 2 (2) ◽  
pp. 92
Author(s):  
Luis Eduardo Téllez Gil ◽  
Elvia María Michelli Viña ◽  
Diana Estela Callejas Monsalve ◽  
Mike Contreras Colmenares ◽  
María Eugenia Cavazza Porro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Viral Infection ◽  
Smoking Habit ◽  
Multiple Infections ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Collection Device ◽  
Dna Collection ◽  
Polymerase Chain

  Las lesiones de cérvix se han asociado a infección por Virus Papiloma Humano (VPH). 300 mujeres mayores de quince años que acudieron al Hospital Universitario de Los Andes (HULA), fueron estudiadas para identificar lesiones, detectar y tipificar VPH, y determinar factores asociados. Se realizó citología, colposcopia, cepillados cervicales utilizando (DNA collection device Digene®) y biopsias en los casos pertinentes. Se aisló el ADN mediante (QIAamp DNA Mini Kit QIAGEN®), siendo cuantificado y almacenado a -20 ºC. Se detectó VPH por Reacción en Cadena de la Polimerasa (PCR) de regiones L1 y E6/E7. La genotipificación por PCR anidada múltiple E6/E7, C. trachomatis se detectó por PCR. El VPH se detectó en 35 % (105) muestras, 88,46 % (92/105) fueron positivas para al menos uno de los genotipos evaluados. VPHAR se encontraron en 97,82 %, (90/92), VPH18 en 82 % (74/90), VPH16 en 44 % (40/90). 56,52 % (52/92) correspondieron a infecciones múltiples, VPH18/16 (20/52) fue la más frecuente. C. trachomatis se detectó en 9 % (27/300) pacientes. La citología mostró cambios sugestivos de infección en solo 16,35 % de las pacientes VPH positivas. 17/18 biopsias sugirieron infección viral y fueron positivas para VPH AR por biología molecular (94,44 %). La colposcopia sugirió infección viral en 46,15 %. El 66,34 % de pacientes fueron menores de 35 años. Se encontró relación estadísticamente no significativa entre infección por VPH, número de parejas sexuales, coinfección con C. trachomatis y hábito tabáquico. Estos resultados muestran elevada frecuencia de infección por VPH AR, asociada a factores epidemiológicos, cuyo diagnóstico certero y tratamiento oportuno son claves en la prevención de su transmisión y del desarrollo de lesiones en cérvix.   Palabras clave: Cáncer cervical, virus papiloma humano, reacción en cadena de la polimerasa.   Abstract   Cervical lesions have been associated with infection by Human Papilloma Virus (HPV). Three hundred women older than 15 years old who attended at the Hospital Universidad de Los Andes (HULA), were studied to identify lesions, detect and typify HPV, and determine associated factors. Cytology, colposcopy, cervical brushing using (DNA collection device Digene®) and biopsies were performed in the pertinent cases. DNA was isolated by (QIAamp DNA Mini Kit QIAGEN®), being quantified and stored at -20 ° C. HPV was detected by Polymerase Chain Reaction (PCR) of regions L1 and E6 / E7. The genotyping by multiple nested PCR E6 / E7, C. trachomatis was detected by PCR. HPV was detected in 35% (105) samples, 88.46% (92/105) were positive for at least one of the genotypes evaluated. VPHAR were found in 97.82% (90/92), HPV18 in 82% (74/90), HPV16 in 44% (40/90). 56.52% (52/92) corresponded to multiple infections, HPV18 / 16 (20/52) was the most frequent. C. trachomatis was detected in 9% (27/300) patients. The cytology showed changes suggestive of infection in only 16.35% of the HPV positive patients. 17/18 biopsies suggested viral infection and were positive for ARV HPV by molecular biology (94.44%). Colposcopy suggested viral infection in 46.15%. 66.34% of patients were under 35 years old. A statistically non-significant relationship was found between HPV infection, number of sexual partners, coinfection with C. trachomatis and smoking habit. These results show high frequency of infection by HPV AR, associated with epidemiological factors, whose accurate diagnosis and timely treatment are key in the prevention of its transmission and the development of lesions in the cervix.   Keywords: Cervical cancer, human papilloma virus, polymerase chain reaction.

scholarly journals Synthesis in vitro and application of biotinylated DNA probes for human papilloma virus type 16 by utilizing the polymerase chain reaction

1990 ◽  
Vol 267 (1) ◽  
pp. 119-123 ◽  
Author(s):  
P J Day ◽  
I S Bevan ◽  
S J Gurney ◽  
L S Young ◽  
M R Walker
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Papilloma Virus ◽  
Virus Type ◽  
Amplification Efficiency ◽  
Human Papilloma Virus Type ◽  
Chain Reaction ◽  
Papilloma Virus ◽  
Hybridization Probes ◽  
Pcr Products ◽  
Polymerase Chain

The polymerase chain reaction (PCR) has been used to incorporate biotinylated deoxynucleotide triphosphate analogues into a 120 bp sequence from the E6 region of human papilloma virus type 16 (HPV 16). No loss of amplification efficiency is observed utilizing concentrations of up to 200 microM-biotin-11-dUTP, or 180 microM-biotin-7-dATP and -biotin-16-dUTP (where the numbers refer to the number of carbon atoms in the spacer arms). Internally biotinylated PCR products can be detected following slot-blot or vacuum transfer to mitrocellulose or nylon filters without prior electrophoretic separation of the reactants, since unincorporated biotinylated analogues pass through the filter. Internally biotinylated PCR products can also be applied as hybridization probes in Southern blot analysis or in situ hybridization. This system enables detection of PCR products or target sequences at levels below that for 5′-biotinylated probes and can be applied in an ‘open sandwich assay’ without the need for a separate labelled probe currently required in conventional sandwich assays. However, as hybridization probes, sensitivity may be limited by the steric hindrance of strand hybridization possibly due to the spacer arms linking the nucleotides to the biotin molecule.

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