It is impossible to keep pipelines free from defects in the manufacturing, installation and servicing processes. In this paper, pre-tension deformation of X60 pipeline steel was employed to experimentally simulate the influence of dents and the mechanism of fatigue crack initiation of X60 pipeline steel after per-tension deformation under cyclic loading were investigated. The results indicate that the mechanism of fatigue crack initiation is the typical cleavage fracture characteristics and the cracks mainly initiates from the non-metallic inclusions which was the local brittle fracture materials such as MnS inclusion. With the pre-tension deformation increase, the yield strength of the matrix was increased and the toughness decreased due to the work-hardening effect. With the effects of the non-metallic inclusions larger, the fatigue cracks initiated from the non-metallic inclusions easier.