Reusing PVDF Electroblotted Protein Samples After N-Terminal Sequencing To Obtain Unique Internal Amino Acid Sequence

1992 ◽  
pp. 61-68 ◽  
Author(s):  
Cynthia L. Wadsworth ◽  
Mark W. Knuth ◽  
Laura W. Burrus ◽  
Bradley B. Olwin ◽  
Ronald L. Niece
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Internal Amino Acid Sequence

A simple in situ cyanogen bromide cleavage method to obtain internal amino acid sequence of proteins electroblotted to polyvinyldifluoride membranes

1988 ◽  
Vol 155 (3) ◽  
pp. 1353-1359 ◽  
Author(s):  
Mitchell G. Scott ◽  
Daniel L. Crimmins ◽  
David W. McCourt ◽  
Jeffrey J. Tarrand ◽  
Margaret C. Eyerman ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Cyanogen Bromide ◽  
Internal Amino Acid Sequence ◽  
Cleavage Method

The staining pattern of the tropomyosin sequence is displayed in a new paracrystal

1986 ◽  
Vol 44 ◽  
pp. 150-151
Author(s):  
M.K. Lamvik ◽  
L.L. Klatt
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Staining Pattern ◽  
Banding Patterns ◽  
Mass Thickness ◽  
New Form

Tropomyosin paracrystals have been used extensively as test specimens and magnification standards due to their clear periodic banding patterns. The paracrystal type discovered by Ohtsuki1 has been of particular interest as a test of unstained specimens because of alternating bands that differ by 50% in mass thickness. While producing specimens of this type, we came across a new paracrystal form. Since this new form displays aligned tropomyosin molecules without the overlaps that are characteristic of the Ohtsuki-type paracrystal, it presents a staining pattern that corresponds to the amino acid sequence of the molecule.

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