Detection of Neuroendocrine Peptide Precursor cDNA Clones Using Synthetic Oligonucleotides

1989 ◽  
pp. 57-65
Author(s):  
JAMES DOUGLASS ◽  
EDWARD HERBERT
Keyword(s):  
Cdna Clones ◽  
Peptide Precursor ◽  
Synthetic Oligonucleotides

Isolation and expression of cDNA clones for a rat liver asialoglycoprotein receptor

1986 ◽  
Vol 6 (6) ◽  
pp. 527-534
Author(s):  
Colin Watts
Keyword(s):  
Rat Liver ◽  
Protein Sequence ◽  
Wheat Germ ◽  
Asialoglycoprotein Receptor ◽  
Cdna Clones ◽  
Oligonucleotide Probes ◽  
Synthetic Oligonucleotide ◽  
Microsomal Membranes ◽  
Synthetic Oligonucleotides

cDNA clones for the major rat liver asialoglycoprotein (ASGP) receptor were isolated from a phage λgtl 1 library using synthetic oligonucleotide probes corresponding to two regions of the protein sequence. The longest clone obtained encoded all but the first 11 codons of the receptor. The cDNA was completed with synthetic oligonucleotides and was used to direct the synthesis of mRNA for the receptor in vitro. Subsequent translation in a wheat germ lysate produced authentic ASGP receptor which assembled correctly into microsomal membranes.

Facilitated Reduction of β-Amyloid Peptide Precursor by Synthetic Oligonucleotides in COS-7 Cells Expressing a Hammerhead Ribozyme

1997 ◽  
Vol 348 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Robert B. Denman ◽  
Marilyn Smedman ◽  
Mathew Abraham ◽  
Mo Chou Chen-Hwang ◽  
Julia R. Currie
Keyword(s):  
Hammerhead Ribozyme ◽  
Amyloid Peptide ◽  
Peptide Precursor ◽  
Β Amyloid ◽  
Β Amyloid Peptide ◽  
Synthetic Oligonucleotides

scholarly journals Isolation and Sequencing of cDNA Clones Coding for Juvenile Hormone Esterase from Heliothis virescens

1989 ◽  
Vol 264 (21) ◽  
pp. 12419-12425 ◽  
Author(s):  
T N Hanzlik ◽  
Y A I Abdel-Aal ◽  
L G Harshman ◽  
B D Hammock
Keyword(s):  
Juvenile Hormone ◽  
Heliothis Virescens ◽  
Cdna Clones ◽  
Juvenile Hormone Esterase
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