Automating Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering

2012 ◽  
pp. 21-42 ◽  
Author(s):  
Melissa Dokarry ◽  
Caroline Laurendon ◽  
Paul E. O'Maille
Keyword(s):  
Protein Engineering ◽  
Gene Library ◽  
Library Synthesis ◽  
Combinatorial Protein Engineering

Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering

2004 ◽  
pp. 75-91 ◽  
Author(s):  
Paul E. O'Maille ◽  
Ming-Daw Tsai ◽  
Bryan T. Greenhagen ◽  
Joseph Chappell ◽  
Joseph P. Noel
Keyword(s):  
Protein Engineering ◽  
Gene Library ◽  
Library Synthesis ◽  
Combinatorial Protein Engineering

Staphylococcal display for combinatorial protein engineering of a head-to-tail affibody dimer binding the Alzheimer amyloid-β peptide

2012 ◽  
Vol 8 (1) ◽  
pp. 139-145 ◽  
Author(s):  
Hanna Lindberg ◽  
Anna Johansson ◽  
Torleif Härd ◽  
Stefan Ståhl ◽  
John Löfblom
Keyword(s):  
Protein Engineering ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Combinatorial Protein Engineering

Structure-based Combinatorial Protein Engineering (SCOPE)

2002 ◽  
Vol 321 (4) ◽  
pp. 677-691 ◽  
Author(s):  
Paul E. O'Maille ◽  
Marina Bakhtina ◽  
Ming-Daw Tsai
Keyword(s):  
Protein Engineering ◽  
Combinatorial Protein Engineering

scholarly journals ANTICALIgN: visualizing, editing and analyzing combined nucleotide and amino acid sequence alignments for combinatorial protein engineering

2016 ◽  
Vol 29 (7) ◽  
pp. 263-270 ◽  
Author(s):  
Alexander Jarasch ◽  
Melanie Kopp ◽  
Evelyn Eggenstein ◽  
Antonia Richter ◽  
Michaela Gebauer ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Engineering ◽  
Amino Acid Sequence ◽  
Sequence Alignments ◽  
Combinatorial Protein Engineering

Rational design and protein engineering of growth factors for regenerative medicine and tissue engineering

2009 ◽  
Vol 37 (4) ◽  
pp. 717-721 ◽  
Author(s):  
Andrew J. Moss ◽  
Shikha Sharma ◽  
Nicholas P.J. Brindle
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Protein Engineering ◽  
Rational Design ◽  
Receptor Activation ◽  
Natural Growth ◽  
Combinatorial Protein Engineering ◽  
Sites Of Action ◽  
Synthetic Growth

Growth factors provide key instructive cues for tissue formation and repair. However, many natural growth factors are limited in their usefulness for tissue engineering and regenerative applications by their poor retention at desired sites of action, short half-lives in vivo, pleiotropic actions and other features. In the present article, we review approaches to rational design of synthetic growth factors based on mechanisms of receptor activation. Such synthetic molecules can function as simplified ligands with potentially tunable specificity and action. Rational and combinatorial protein engineering techniques allow introduction of additional features into these synthetic growth molecules, as well as natural growth factors, which significantly enhance their therapeutic utility.

Staphylococcal Surface Display in Combinatorial Protein Engineering and Epitope Mapping of Antibodies

2010 ◽  
Vol 4 (3) ◽  
pp. 171-182 ◽  
Author(s):  
Nina Kronqvist ◽  
Magdalena Malm ◽  
Johan Rockberg ◽  
Barbara Hjelm ◽  
Mathias Uhlen ◽  
...  
Keyword(s):  
Protein Engineering ◽  
Epitope Mapping ◽  
Surface Display ◽  
Combinatorial Protein Engineering

scholarly journals Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy

2016 ◽  
Vol 473 (10) ◽  
pp. 1329-1341 ◽  
Author(s):  
Itay Cohen ◽  
Olumide Kayode ◽  
Alexandra Hockla ◽  
Banumathi Sankaran ◽  
Derek C. Radisky ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Protein Engineering ◽  
Morbidity And Mortality ◽  
New Therapies ◽  
Combinatorial Protein Engineering ◽  
Pancreatic Cancers ◽  
The Way

Cancer is a leading cause of morbidity and mortality worldwide. The results presented in the present study pave the way to develop new therapies targeting mesotrypsin, an enzyme that contributes to progression and metastasis of lung, prostate, breast and pancreatic cancers.

scholarly journals Combinatorial protein engineering by incremental truncation

1999 ◽  
Vol 96 (7) ◽  
pp. 3562-3567 ◽  
Author(s):  
M. Ostermeier ◽  
A. E. Nixon ◽  
J. H. Shim ◽  
S. J. Benkovic
Keyword(s):  
Protein Engineering ◽  
Combinatorial Protein Engineering ◽  
Incremental Truncation

scholarly journals Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries

2018 ◽  
Vol 14 ◽  
pp. 397-406 ◽  
Author(s):  
Ruth Suchsland ◽  
Bettina Appel ◽  
Sabine Müller
Keyword(s):  
Protein Engineering ◽  
Dna Synthesis ◽  
Solid Phase ◽  
Gene Synthesis ◽  
Gene Library ◽  
Library Preparation ◽  
Soluble Polymers ◽  
Gene Libraries ◽  
Recent Developments ◽  
Protein Libraries

The preparation of protein libraries is a key issue in protein engineering and biotechnology. Such libraries can be prepared by a variety of methods, starting from the respective gene library. The challenge in gene library preparation is to achieve controlled total or partial randomization at any predefined number and position of codons of a given gene, in order to obtain a library with a maximum number of potentially successful candidates. This purpose is best achieved by the usage of trinucleotide synthons for codon-based gene synthesis. We here review the strategies for the preparation of fully protected trinucleotides, emphasizing more recent developments for their synthesis on solid phase and on soluble polymers, and their use as synthons in standard DNA synthesis.

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