BamHI, HindIII, and EcoRI Restriction Endonuclease Cleavage Sites in the argECBH Region of the Escherichia coli Chromosome

1977 ◽  
pp. 273-278
Author(s):  
MAR.C. MORAN ◽  
ANTHON.J. MAZAITIS ◽  
RUT.H. VOGEL ◽  
HENR.J. VOGEL
Keyword(s):  
Escherichia Coli ◽  
Restriction Endonuclease ◽  
Cleavage Sites ◽  
Ecori Restriction ◽  
Restriction Endonuclease Cleavage

Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei

1982 ◽  
Vol 2 (7) ◽  
pp. 845-852
Author(s):  
K D Stuart ◽  
S B Gelvin
Keyword(s):  
Restriction Endonuclease ◽  
Trypanosoma Brucei ◽  
Variable Region ◽  
Cleavage Sites ◽  
Kinetoplast Dna ◽  
Coding Sequences ◽  
Procyclic Form ◽  
Restriction Endonuclease Cleavage ◽  
Mitochondrial Respiratory

Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.

scholarly journals Localization of kinetoplast DNA maxicircle transcripts in bloodstream and procyclic form Trypanosoma brucei.

1982 ◽  
Vol 2 (7) ◽  
pp. 845-852 ◽  
Author(s):  
K D Stuart ◽  
S B Gelvin
Keyword(s):  
Restriction Endonuclease ◽  
Trypanosoma Brucei ◽  
Variable Region ◽  
Cleavage Sites ◽  
Kinetoplast Dna ◽  
Coding Sequences ◽  
Procyclic Form ◽  
Restriction Endonuclease Cleavage ◽  
Mitochondrial Respiratory

Over 80% of the maxicircle and numerous minicircles of Trypanosoma brucei kinetoplast DNA have been cloned. The uncloned maxicircle segment contains few restriction endonuclease cleavage sites, varies in size among strains, and may be unstable in conventional cloning systems. cDNA prepared to bloodstream or procyclic trypomastigote RNA hybridized to all but one maxicircle segment, but did not hybridize to minicircles. Fourteen maxicircle transcripts were detected in RNA from both bloodstream and procyclic trypomastigotes. The coding sequences for these transcripts were localized and account for most of the maxicircle. One region of the maxicircle, which borders the variable region, was not found to be transcribed. We conclude that the maxicircle is largely but not completely transcribed in both bloodstream and procyclic trypomastigotes, whereas minicircle transcription is minimal or absent in these stages. Qualitative transcriptional differences which could account for mitochondrial respiratory differences between the bloodstream and procyclic trypomastigotes were not observed.

Linkage of heat-stable enterotoxin activity and ampicillin resistance in a plasmid isolated from an Escherichia coli strain of human origin

1980 ◽  
Vol 30 (2) ◽  
pp. 617-620
Author(s):  
H Stieglitz ◽  
R Fonseca ◽  
J Olarte ◽  
Y M Kupersztoch-Portnoy
Keyword(s):  
Escherichia Coli ◽  
Genetic Analysis ◽  
Restriction Endonuclease ◽  
Coli Strain ◽  
Human Origin ◽  
Ampicillin Resistance ◽  
Heat Stable ◽  
Ecori Restriction

In an Escherichia coli strain of human origin, ampicillin resistance and heat-stable enterotoxin activity were shown by EcoRI restriction endonuclease and genetic analysis to be in an 80-megadalton plasmid.

RNA and homology mapping of two DNA fragments with repressible acid phosphatase genes from Saccharomyces cerevisiae

1983 ◽  
Vol 3 (4) ◽  
pp. 562-569
Author(s):  
N Andersen ◽  
G P Thill ◽  
R A Kramer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acid Phosphatase ◽  
Restriction Endonuclease ◽  
Heteroduplex Analysis ◽  
Dna Fragments ◽  
Restriction Fragments ◽  
Ecori Restriction ◽  
Restriction Endonuclease Cleavage

Two EcoRI restriction fragments carrying Saccharomyces cerevisiae repressible acid phosphatase genes were analyzed. Transcripts were mapped by restriction endonuclease cleavage of glyoxal-stabilized R-loops and by gel blot hybridizations to cDNA. Homology between the two fragments was examined by gel blots and heteroduplex analysis. Each fragment carried a region of about 1.5 kilobases that coded for a repressible acid phosphatase, and these regions showed homology to one another. In addition, one fragment carried a second region of somewhat lower homology that probably codes for the so-called constitutive acid phosphatase.

Survey and mapping of restriction endonuclease cleavage sites in bacteriophage T7 DNA

1979 ◽  
Vol 135 (4) ◽  
pp. 907-915 ◽  
Author(s):  
Alan H. Rosenberg ◽  
Martha N. Simon ◽  
F.William Studier ◽  
Richard J. Roberts
Keyword(s):  
Restriction Endonuclease ◽  
Bacteriophage T7 ◽  
Cleavage Sites ◽  
Restriction Endonuclease Cleavage
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