Global analysis of protein stability by temperature and chemical denaturation

AbstractNMR-spectroscopy has certain unique advantages for recording unfolding transitions of proteins compared e.g. to optical methods. It enables per-residue monitoring and separate detection of the folded and unfolded state as well as possible equilibrium intermediates. This allows a detailed view on the state and cooperativity of folding of the protein of interest and the correct interpretation of subsequent experiments. Here we summarize in detail practical and theoretical aspects of such experiments. Certain pitfalls can be avoided, and meaningful simplification can be made during the analysis. Especially a good understanding of the NMR exchange regime and relaxation properties of the system of interest is beneficial. We show by a global analysis of signals of the folded and unfolded state of GB1 how accurate values of unfolding can be extracted and what limits different NMR detection and unfolding methods. E.g. commonly used exchangeable amides can lead to a systematic under determination of the thermodynamic protein stability. We give several perspectives of how to deal with more complex proteins and how the knowledge about protein stability at residue resolution helps to understand protein properties under crowding conditions, during phase separation and under high pressure.

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Global analysis of the thermal and chemical denaturation of the N-terminal domain of the ribosomal protein L9 in H2O and D2O. Determination of the thermodynamic parameters, ΔH°, ΔS°, and ΔC°p, and evaluation of solvent isotope effects

Protein Science ◽

10.1002/pro.5560071118 ◽

1998 ◽

Vol 7 (11) ◽

pp. 2405-2412 ◽

Cited By ~ 51

Author(s):

Brian Kuhlman ◽

Daniel P. Raleigh

Keyword(s):

Ribosomal Protein ◽

Thermodynamic Parameters ◽

Global Analysis ◽

Isotope Effects ◽

Chemical Denaturation ◽

Terminal Domain ◽

Ribosomal Protein L9 ◽

Solvent Isotope ◽

Solvent Isotope Effects

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Global Analysis of the Mitochondrial N-Proteome Identifies a Processing Peptidase Critical for Protein Stability

Cell ◽

10.1016/j.cell.2009.07.045 ◽

2009 ◽

Vol 139 (2) ◽

pp. 428-439 ◽

Cited By ~ 302

Author(s):

F.-Nora Vögtle ◽

Stefanie Wortelkamp ◽

René P. Zahedi ◽

Dorothea Becker ◽

Claudia Leidhold ◽

...

Keyword(s):

Protein Stability ◽

Global Analysis ◽

Processing Peptidase

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Faculty Opinions recommendation of Global analysis of the mitochondrial N-proteome identifies a processing peptidase critical for protein stability.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1165899.628986 ◽

2009 ◽

Author(s):

Martin Pool

Keyword(s):

Protein Stability ◽

Global Analysis ◽

Processing Peptidase

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Introduction to Global Analysis

10.1016/s0079-8169(08)x6071-4 ◽

1980 ◽

Keyword(s):

Global Analysis

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Local and global analysis of plasticity in ferritic-austenitic duplex steels

Journal de Physique IV (Proceedings) ◽

10.1051/jp4:2001536 ◽

2001 ◽

Vol 11 (PR5) ◽

pp. Pr5-293-Pr5-300 ◽

Cited By ~ 1

Author(s):

V. V. Silberschmidt ◽

M. Ortmayr ◽

C. Messner ◽

E. A. Werner

Keyword(s):

Global Analysis ◽

Duplex Steels

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Global Analysis of Host Tissue Gene Expression in the Invasive Front of Colorectal Liver Metastases

Zeitschrift für Gastroenterologie ◽

10.1055/s-2005-861618 ◽

2005 ◽

Vol 43 (01) ◽

Author(s):

K Brand ◽

OR Bandapalli ◽

C Franke ◽

D Kobelt ◽

K Kühnle ◽

...

Keyword(s):

Gene Expression ◽

Liver Metastases ◽

Colorectal Liver Metastases ◽

Global Analysis ◽

Host Tissue ◽

Invasive Front ◽

Tissue Gene Expression

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Do remittances differ depending on migration pathway and length of stay?

REMITTANCES REVIEW ◽

10.33182/rr.v1i1.445 ◽

2016 ◽

Vol 1 (1) ◽

pp. 105-118

Author(s):

Anita Pugliese ◽

Julie Ray ◽

Neli Esipova

Keyword(s):

Length Of Stay ◽

First Generation ◽

Second Generation ◽

Global Analysis ◽

Large Sample ◽

Migration Pathway ◽

First Generation Migrants ◽

Second Generation Migrants

This paper reports the results from Gallup’s global analysis of the likelihood of first-generation migrants, second-generation migrants and the native-born to send financial help in the form of money or goods to others inside or outside their respective country of residence. The findings in this paper are based on more than 450,000 interviews conducted through Gallup’s World Poll in 157 countries in 2012, 2013 and 2014. The sample includes more than 26,000 first-generation migrants and more than 20,000 second-generation migrants. The large sample enables Gallup to analyze first-generation migrants by the duration of their stay in their adopted country and compare their remittance behaviors with second-generation migrants and the native-born.

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