Simultaneous determination of zearalenone and ochratoxin A based on microscale thermophoresis assay with a bifunctional aptamer

2021 ◽  
Vol 1155 ◽  
pp. 338345
Author(s):  
Yan Yang ◽  
Yingai Yin ◽  
Sai Wang ◽  
Yiyang Dong
Keyword(s):  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Microscale Thermophoresis

Development and validation of an LC–MS/MS method for the simultaneous determination of citrinin and ochratoxin a in a variety of feed and foodstuffs

2018 ◽  
Vol 1580 ◽  
pp. 100-109 ◽  
Author(s):  
Celine Meerpoel ◽  
Arnau Vidal ◽  
José Diana di Mavungu ◽  
Bart Huybrechts ◽  
Emmanuel K. Tangni ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Development And Validation

scholarly journals Simultaneous Determination of Twenty Mycotoxins in the Korean Soybean Paste Doenjang by LC-MS/MS with Immunoaffinity Cleanup

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 594 ◽  
Author(s):  
So Young Woo ◽  
So Young Ryu ◽  
Fei Tian ◽  
Sang Yoo Lee ◽  
Su Been Park ◽  
...  
Keyword(s):  
Measurement Uncertainty ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Matrix Effect ◽  
Contamination Level ◽  
Immunoaffinity Column ◽  
Good Linearity ◽  
Soybean Paste ◽  
Contamination Levels

Doenjang, a Korean fermented soybean paste, is vulnerable to contamination by mycotoxins because it is directly exposed to environmental microbiota during fermentation. A method that simultaneously determines 20 mycotoxins in doenjang, including aflatoxins (AFs), ochratoxin A (OTA), zearalenone (ZEN), and fumonisins (FBs) with an immunoaffinity column cleanup was optimized and validated in doenjang using LC-MS/MS. The method showed good performance in the analysis of 20 mycotoxins in doenjang with good linearity (R2 > 0.999), intra- and inter-day precision (<16%), recovery (72–112%), matrix effect (87–104%), and measurement uncertainty (<42%). The validated method was applied to investigate mycotoxin contamination levels in commercial and homemade doenjang. The mycotoxins that frequently contaminated doenjang were AFs, OTA, ZEN, and FBs and the average contamination level and number of co-occurring mycotoxins in homemade doenjang were higher than those in commercially produced doenjang.

Method Validation for the Quantitative Analysis of Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in Processed Cereal-Based Foods by HPLC with Fluorescence Detection

2015 ◽  
Vol 98 (4) ◽  
pp. 939-945 ◽  
Author(s):  
Işil Gazioğlu ◽  
Ufuk Kolak
Keyword(s):  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Aflatoxin B1 ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Immunoaffinity Column ◽  
Rp Hplc

Abstract Modified AOAC 991.31 and AOAC 2000.03 methods for the simultaneous determination of total aflatoxins (AFs), aflatoxin B1, and ochratoxin A (OTA) in processed cereal-based foods by RP-HPLC coupled with fluorescence detection were validated. A KOBRA® Cell derivatization system was used to analyze total AFs. One of the modifications was the extraction procedure of mycotoxins. Both AFs and OTA were extracted with methanol–water (75 + 25, v/v) and purified with an immunoaffinity column before HPLC analysis. The modified methods were validated by measuring the specificity, selectivity, linearity, sensitivity, accuracy, repeatability, reproducibility, recovery, LOD, and LOQ parameters. The validated methods were successfully applied for the simultaneous determination of mycotoxins in 81 processed cereal-based foods purchased in Turkey. These rapid, sensitive, simple, and validated methods are suitable for the simultaneous determination of AFs and OTA in the processed cereal-based foods.

Simultaneous determination of aflatoxin B1and ochratoxin A and their natural occurrence in Mediterranean virgin olive oil

2007 ◽  
Vol 24 (2) ◽  
pp. 173-180 ◽  
Author(s):  
R. Ferracane ◽  
A. Tafuri ◽  
A. Logieco ◽  
F. Galvano ◽  
D. Balzano ◽  
...  
Keyword(s):  
Olive Oil ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Virgin Olive Oil ◽  
Natural Occurrence

Simultaneous Determination of Aflatoxins and Ochratoxin A in Pork by LC-MS/MS

2016 ◽  
Vol 31 (3) ◽  
pp. 194-200
Author(s):  
Ockjin Paek ◽  
◽  
Songyi Park ◽  
Ki Hun Park ◽  
Sheen-Hee Kim ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Ochratoxin A

Simultaneous Determination of Aflatoxins and Ochratoxin A in Bee Pollen by Low-Temperature Fat Precipitation and Immunoaffinity Column Cleanup Coupled with LC-MS/MS

2013 ◽  
Vol 7 (3) ◽  
pp. 690-696 ◽  
Author(s):  
XiaoFeng Xue ◽  
Jonathan Nimal Selvaraj ◽  
Liuwei Zhao ◽  
Haimin Dong ◽  
Fengmao Liu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Immunoaffinity Column ◽  
Bee Pollen

scholarly journals Simultaneous Determination of Aflatoxins, Ochratoxin A, and Zearalenone in Grains by New Immunoaffinity Column/Liquid Chromatography

2004 ◽  
Vol 87 (2) ◽  
pp. 411-416 ◽  
Author(s):  
Roswitha Göbel ◽  
Klaus Lusky
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Trifluoroacetic Acid ◽  
Detection Limits ◽  
Immunoaffinity Column ◽  
Recovery Rates

Abstract The simultaneous determination of mycotoxins was performed in 3 steps: extraction, cleanup, and detection. For extraction, a mixture of acetonitrile–water (60 + 40, v/v) was proved appropriate. For cleanup, a new Afla-Ochra-Zea immunoaffinity column was used. After derivatization with trifluoroacetic acid, the mycotoxins aflatoxins, ochratoxin A (OTA), and zearalenone (ZEA) were determined simultaneously by liquid chromatography with fluorescence detection. The detection limits in different matrixes after cleanup with the new immunoaffinity column were very low: aflatoxins, 0.002–0.7 μg/kg; OTA, 0.07–0.25 μg/kg; ZEA, 1–3 μg/kg. The limits of determination were: aflatoxins, 0.25 μg/kg; OTA, 0.5 μg/kg; ZEA, 5 μg/kg. The recovery rates for aflatoxins, OTA, and ZEA for rye and rice were between 86 and 93% when a 0.5 g sample matter per immunoaffinity column was used.

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