Self-assembling peptides optimize the post-traumatic milieu and synergistically enhance the effects of neural stem cell therapy after cervical spinal cord injury

Spinal cord injury (SCI) is a common and potentially life-threatening condition with no established treatment to treat the primary injury. Mesenteric neural stem cell (NSC) therapy is a promising stem cell therapy to treat primary SCI in the chronic phase. We aimed to review the literature narratively to describe current evidence regarding mesenteric NSC in SCI. Primary SCI refers to tissue damage that occurs at the time of trauma that leads to the death of neuronal cells. In chronic SCI, the ability of neuronal regeneration is compromised by the development of gliotic scar. NSC is a stem cell therapy that targeted SCI in the chronic phase. Enteric NSC is one of the sources of NSC, and autologous gut harvesting in the appendix using endoscopic surgery provides a more straightforward and low-risk procedure. Intramedullary transplantation of stem cell with ultrasound guiding is administration technique which offers long-term regeneration. Mesenteric NSC is a promising stem cell therapy to treat chronic SCI with low risk and easier procedure to isolate cells compared to other NSC sources.

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Genetically-Engineered Neural Stem Cell Therapy for Spinal Cord Injury

Spinal Surgery ◽

10.2531/spinalsurg.25.84 ◽

2011 ◽

Vol 25 (1) ◽

pp. 84-87

Author(s):

Yusuke Nishimura ◽

Atsusi Natsume ◽

Masahito Hara ◽

Kazuya Motomura ◽

Toshihiko Wakabayashi

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Stem Cell ◽

Cell Therapy ◽

Neural Stem Cell ◽

Stem Cell Therapy ◽

Genetically Engineered ◽

Cord Injury

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Alterations in the expression of the apurinic/apyrimidinic endonuclease-1/redox factor-1 (APE/ref-1) and DNA damage in the caudal region of acute and chronic spinal cord injured rats treated by embryonic neural stem cells

Physiological Research ◽

10.33549/physiolres.931388 ◽

2009 ◽

pp. 427-434

Author(s):

T Dagci ◽

G Armagan ◽

S Konyalioglu ◽

A Yalcin

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Dna Damage ◽

Stem Cell ◽

Cell Therapy ◽

Neural Stem Cell ◽

Stem Cell Therapy ◽

Mrna Levels ◽

Cord Injury ◽

Embryonic Neural Stem Cell

The oxidative mechanisms of injury-induced damage of neurons within the spinal cord are not very well understood. We used a model of T8-T9 spinal cord injury (SCI) in the rat to induce neuronal degeneration. In this spinal cord injury model, unilateral avulsion of the spinal cord causes oxidative stress of neurons. We tested the hypothesis that apurinic/apyrimidinic endonuclease (or redox effector factor-1, APE/Ref-1) regulates this neuronal oxidation mechanism in the spinal cord region caudal to the lesion, and that DNA damage is an early upstream signal. The embryonic neural stem cell therapy significantly decreased DNAdamage levels in both study groups – acutely (followed up to 7 days after SCI), and chronically (followed up to 28 days after SCI) injured animals. Meanwhile, mRNA levels of APE/Ref-1 significantly increased after embryonic neural stem cell therapy in acutely and chronically injured animals when compared to acute and chronic sham groups. Our data has demonstrated that an increase of APE/Ref-1 mRNA levels in the caudal region of spinal cord strongly correlated with DNA damage after traumatic spinal cord injury. We suggest that DNA damage can be observed both in lesional and caudal regions of the acutely and chronically injured groups, but DNA damage is reduced with embryonic neural stem cell therapy.

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