Ultrastructural localization of desmoglein and plakophilin in the human hair suggests that the cell membrane complex is a long desmosomal remnant

2013 ◽  
Vol 115 (8) ◽  
pp. 879-886 ◽  
Author(s):  
Lorenzo Alibardi ◽  
Masaru Tsuchiya ◽  
Shunichi Watanabe ◽  
Bernd Nöcker
Keyword(s):  
Cell Membrane ◽  
Human Hair ◽  
Ultrastructural Localization ◽  
Membrane Complex ◽  
Cell Membrane Complex

High-voltage Electron Microscopy and 3-D reconstruction of human hair fiber ultrastructure

1989 ◽  
Vol 47 ◽  
pp. 116-117
Author(s):  
L. Rudy ◽  
R. Sneath ◽  
M. Song
Keyword(s):  
Electron Microscopy ◽  
Cell Membrane ◽  
High Voltage ◽  
Human Hair ◽  
Three Dimensional ◽  
High Voltage Electron Microscopy ◽  
Membrane Complex ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
Cell Membrane Complex

The basic morphology of the non-keratinous regions of human hair fibers was studied using both conventional and high voltage electron microscopy. The non-keratinous regions of hair include the endocuticle, the cell membrane complex, and the nuclear remnants of the cortex. By characterizing these regions more clearly, the mechanisms by which external influences affect the hair can be understood. The nuclear remnants are surrounded by a cell membrane complex. Since thin sectioning often causes artifacts in these fragile structures, a three-dimensional reconstruction using serial, semi-thick sections was completed to reveal their morphological nature.Human hair fibers collected from a female subject, had not been treated with any chemically active processes. One centimeter samples were collected near the scalp region of the back of the head. The fibers were embedded in Epon-812. Serial, semi-thick sections, 0.25u thick, were sectioned and collected on copper slot, formvar-coated grids. Post-staining was completed with uranyl acetate and lead citrate. Sections were examined in an AEl EM7 Mk 1.2MV HVEM at an accelerating voltage of 1.0 MV.

scholarly journals Microdiffraction analysis of cell membrane complex (CMC) structure in human hair

2003 ◽  
Vol 43 (supplement) ◽  
pp. S171
Author(s):  
N. Ohta ◽  
T. Oka ◽  
K. Inoue ◽  
N. Yagi ◽  
S. Kato ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Human Hair ◽  
Membrane Complex ◽  
Cell Membrane Complex

Structural analysis of cell membrane complex of a hair fibre by micro-beam X-ray diffraction

2005 ◽  
Vol 38 (2) ◽  
pp. 274-279 ◽  
Author(s):  
Noboru Ohta ◽  
Toshihiko Oka ◽  
Katsuaki Inoue ◽  
Naoto Yagi ◽  
Satoru Kato ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Small Angle ◽  
Human Hair ◽  
Structural Information ◽  
X Ray Diffraction ◽  
Diffraction Profile ◽  
X Ray ◽  
Membrane Complex ◽  
Hair Fibre ◽  
Cell Membrane Complex

The cell membrane complex in the cuticle of a human hair fibre or a rat whisker is composed of three layers, that is, β, δ and β layers. The X-ray diffraction technique is a powerful tool to investigate the pathway of aqueous molecules and ions across the cuticle. Small-angle scattering experiments using a micro X-ray beam, which can be applied to a cuticle of 5 µm thickness, provide the structural information on the cell membrane complex without interference from other structures. Taking into account the variation of thickness in the δ and β layers, the overall features of the diffraction profile in a small-angle region can be explained satisfactorily. The method makes it possible to analyse the structure of β, δ and β layers without assuming an ambiguous background in the diffraction profile, and was used for the analysis of a human hair fibre and a rat whisker. In a rat whisker, the X-ray diffraction was stronger and the variation in the layer thickness smaller than in a human hair fibre. This may be due to the fact that the rat whisker had not been washed with soap or cosmetically treated, whereas the variation may depend on the lipids or the proteins that each species naturally has. It is proposed that the method represents convenient tool for quantitative analysis to estimate the thickness of δ and β layers in the cell membrane complex.

New Advances in the Internal Lipid Composition of Wool

1988 ◽  
Vol 58 (6) ◽  
pp. 338-342 ◽  
Author(s):  
B. Nogues ◽  
L. Coderch ◽  
R. Julia ◽  
P. Erra
Keyword(s):  
Cell Membrane ◽  
Organic Solvents ◽  
Lipid Composition ◽  
Bilayer Structure ◽  
Membrane Complex ◽  
Wool Fibers ◽  
Cell Membrane Complex

The presence of glycolipids on wool fibers has been detected by analyzing the solubilized material when wool is treated with different organic solvents selected to remove internal lipids. One of these has been isolated and identified as a glucosilceramide. These compounds are thought to contribute to the bilayer structure that may be present in the β-layers of the cell membrane complex.

scholarly journals Histochemical observation of the cell membrane complex of hair.

1992 ◽  
Vol 48 (8) ◽  
pp. 420-426 ◽  
Author(s):  
Sachio Naito ◽  
Toshie Takahashi ◽  
Michihiro Hattori ◽  
Kozo Arai
Keyword(s):  
Cell Membrane ◽  
Membrane Complex ◽  
Histochemical Observation ◽  
Cell Membrane Complex

scholarly journals The Chemical Composition of Wool XV. The Cell Membrane Complex

1976 ◽  
Vol 29 (2) ◽  
pp. 43 ◽  
Author(s):  
DE Peters ◽  
JH Bradbury
Keyword(s):  
Cell Membrane ◽  
Cross Sections ◽  
Performic Acid ◽  
Appreciable Amount ◽  
Lysine Content ◽  
Cortical Cells ◽  
Membrane Complex ◽  
Chemical Attack ◽  
Cross Links ◽  
Cell Membrane Complex

The cell membrane complex of wool has been examined by electron microscopy of stained. cross sections after immersion of the wool in formic acid. The cell membrane complex of the cortex is considerably modified by the treatment, but that of the cuticle appears unchanged. Resistant membranes from cuticle cells, cortical cells and wool have been prepared by treatment with performic acid-ammonia. Amino acid analyses show that the resistant membranes from the cuticle contain citrulline but those from cortical cells do not. It is concluded that the cell membrane complex of the cuticle differs from that of the cortex. Because of the high lysine content of the resistant membranes, their resistance to chemical attack, the hydrophobicity of epicuticle and the observation of a small amount of e-(y-glutamyl)lysine, it is postulated that the resistant membranes may contain an appreciable amount of e-(y-glutamyl)lysine cross links.

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