Identification of best housekeeping genes for the normalization of RT-qPCR in human cell lines

2022 ◽  
Vol 124 (1) ◽  
pp. 151821
Author(s):  
Leticia da Conceição Braga ◽  
Bryan Ôrtero Perez Gonçalves ◽  
Pollyanne Lacerda Coelho ◽  
Agnaldo Lopes da Silva Filho ◽  
Luciana Maria Silva
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Housekeeping Genes ◽  
Human Cell Lines

scholarly journals Lariat intronic RNAs in the cytoplasm of vertebrate cells

2018 ◽  
Vol 115 (34) ◽  
pp. E7970-E7977 ◽  
Author(s):  
Gaëlle J. S. Talhouarne ◽  
Joseph G. Gall
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Biological Significance ◽  
Housekeeping Genes ◽  
Passive Diffusion ◽  
Human Cell Lines ◽  
Intronic Sequence

Most intronic RNAs are degraded within seconds or minutes after their excision from newly formed transcripts. However, stable intronic sequence RNAs (sisRNAs) have been described from oocytes of the frog Xenopus, from Drosophila embryos, and from human cell lines. In Xenopus oocytes, sisRNAs are abundant in both the nucleus and cytoplasm, they occur in the form of lariats, and they are stable for days. In this study we demonstrate that cytoplasmic sisRNAs are also found in human, mouse, chicken, and zebrafish cells. They exist as circular (lariat) molecules, mostly 100–500 nucleotides in length, and are derived from many housekeeping genes. They tend to have an unusual cytosine branchpoint (with the exception of those from the frog). Stable lariats are exported from the nucleus to the cytoplasm by the NXF1/NXT1 system, demonstrating that their presence in the cytoplasm is not due to passive diffusion. Lariats in the cytoplasm are not associated with transcripts of the genes from which they are derived. The biological significance of cytoplasmic sisRNAs remains obscure.

Investigations of the influence of microgravity on the state of human cell lines

2004 ◽  
Vol 10 (5-6) ◽  
pp. 226-228
Author(s):  
L.M. Nosach ◽  
◽  
O.Yu. Povnitsa ◽  
V.L. Zhovnovata ◽  
◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
The State ◽  
Human Cell Lines

scholarly journals Dicalcin suppresses in vitro trophoblast attachment in human cell lines

2021 ◽  
Vol 570 ◽  
pp. 206-213
Author(s):  
Ryohei Saito ◽  
Hiromasa Satoh ◽  
Kayo Aoba ◽  
Hajime Hirasawa ◽  
Naofumi Miwa
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines

scholarly journals Differential Effects of Cyanidin and Cyanidin-3-glucoside on Human Cell Lines

2011 ◽  
Vol 17 (6) ◽  
pp. 515-521 ◽  
Author(s):  
Masayuki TAKEUCHI ◽  
Katsuki OHTANI ◽  
Yanju MA ◽  
Sanae KATO ◽  
Shingo SEMBA ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Differential Effects
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