Enhanced H2 production with efficient N2-fixation by fructose mixotrophically grown Anabaena sp. PCC 7120 strain disrupted in uptake hydrogenase

2020 ◽  
Vol 47 ◽  
pp. 101823
Author(s):  
Wanthanee Khetkorn ◽  
Peter Lindblad ◽  
Aran Incharoensakdi
Keyword(s):  
N2 Fixation ◽  
H2 Production ◽  
Uptake Hydrogenase ◽  
Anabaena Sp ◽  
Pcc 7120

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Target Gene Inactivation in Cyanobacterium Anabaena sp. PCC 7120

BIO-PROTOCOL ◽  
2016 ◽  
Vol 6 (15) ◽  
Author(s):  
Kangming Chen ◽  
Huilan Zhu ◽  
Liping Gu ◽  
Shengni Tian ◽  
Ruanbao Zhou
Keyword(s):  
Target Gene ◽  
Gene Inactivation ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

scholarly journals 2‐oxoglutarate modulates the affinity of FurA for the ntcA promoter in Anabaena sp. PCC 7120

FEBS Letters ◽  
2019 ◽  
Vol 594 (2) ◽  
pp. 278-289
Author(s):  
Jorge Guío ◽  
Cristina Sarasa‐Buisan ◽  
Adrián Velázquez‐Campoy ◽  
María Teresa Bes ◽  
María F. Fillat ◽  
...  
Keyword(s):  
Anabaena Sp ◽  
Pcc 7120

scholarly journals Cloning, sequencing and transcriptional studies of the genes for cytochrome c-553 and plastocyanin from Anabaena sp. PCC 7120

Microbiology ◽  
1994 ◽  
Vol 140 (5) ◽  
pp. 1151-1159 ◽  
Author(s):  
M. Ghassemian ◽  
B. Wong ◽  
F. Ferreira ◽  
J. L. Markley ◽  
N. A. Straus
Keyword(s):  
Cytochrome C ◽  
Anabaena Sp ◽  
Pcc 7120
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