scholarly journals ACPM Member Spotlight: Maria Mora Pinzon, MD, MS, FACPM, Assistant Scientist, Research Fellow, University of Wisconsin - Madison

2021 ◽  
Vol 61 (2) ◽  
pp. 308-309
Author(s):  
Donna Grande
Author(s):  
Hans Ris

The High Voltage Electron Microscope Laboratory at the University of Wisconsin has been in operation a little over one year. I would like to give a progress report about our experience with this new technique. The achievement of good resolution with thick specimens has been mainly exploited so far. A cold stage which will allow us to look at frozen specimens and a hydration stage are now being installed in our microscope. This will soon make it possible to study undehydrated specimens, a particularly exciting application of the high voltage microscope.Some of the problems studied at the Madison facility are: Structure of kinetoplast and flagella in trypanosomes (J. Paulin, U. of Georgia); growth cones of nerve fibers (R. Hannah, U. of Georgia Medical School); spiny dendrites in cerebellum of mouse (Scott and Guillery, Anatomy, U. of Wis.); spindle of baker's yeast (Joan Peterson, Madison) spindle of Haemanthus (A. Bajer, U. of Oregon, Eugene) chromosome structure (Hans Ris, U. of Wisconsin, Madison). Dr. Paulin and Dr. Hanna are reporting their work separately at this meeting and I shall therefore not discuss it here.


Author(s):  
Patricia N. Hackney

Ustilago hordei and Ustilago violacea are yeast-like basidiomycete pathogens ofHordeum vulgare and Silene alba respectively. The mating type system in both species of Ustilago is bipolar, with alleles, A,a, (U.hordei) and a1, a2 (U.violacea) at a single locus. Haploid sporidia maintain the asexual phase by budding, while the sexual phase is initiated by conjugation tube formation between the mating types during budding and conjugation.For observation of budding, sporidia were prepared by culturing the four types on YEG (yeast extract glucose) broth for 24 hours. After centrifugation at 5000g cells were either left unmated or mated in a1/a2,A/a combinations. The sporidia were then mixed 1:1 with 4% agar and the resulting 1mm cubes fixed in 8% gluteraldehyde and post fixed in osmium tetroxide. After dehydration and embedding cubes were thin sectioned with a LKB ultratome and photographed in a Zeiss 9s transmission electron microscope or in an AE1 electron microscope of MK11 1MEV at the High Voltage Electron Microscopy Center of the University of Wisconsin-Madison.


2019 ◽  
Vol 16 (2) ◽  
pp. 341-344
Author(s):  
Rana Aytug ◽  
Lee Daly

On December 6, 2018, academics from across Europe and beyond gathered at London’s Freud Museum for a conference on the politics, policies and diplomacy of diaspora governance. This conference was organized by Senior Research Fellow Dr Bahar Baser from the Centre for Trust, Peace and Social Relations (CTPSR), Coventry University, UK and Dr Henio Hoyo, Research Professor from the Department of Social Sciences/School of Law and Social Sciences, Universidad de Monterrey (UDEM) Monterrey, Mexico.


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