Effects of rumen-protected arginine supplementation on the plasma amino acids and gut microbiota of sika deer (Cervus nippon)

Velvet antler is a regeneration organ of sika deer (Cervus nippon) and an important Chinese medicine, and nutrient metabolism affects its growth. Here, we investigated the effects of arginine supplementation on antler growth, serum biochemical indices, and the rumen bacterial community of sika deer during the antler growth period. Fifteen male sika deer (6 years old) were randomly assigned to three dietary groups, which were supplemented with 0 (n = 5, CON), 2.5 (n = 5, LArg), or 5.0 g/d (n = 5, HArg) L-arginine. The IGF-1, ALT and AST concentrations in the serum of LArg sika deer were significantly higher than those in the serum of CON (P < 0.05) and HArg deer (P < 0.05). The phyla Bacteroidetes, Firmicutes, and Proteobacteria were dominant in the rumen of sika deer among the three groups. Comparison of alpha diversities showed that the ACE and Chao1 indices significantly increased in the LArg and HArg groups compared with those in the CON group. PCoA and ANOSIM results showed that the bacterial community was significantly changed between the CON and LArg groups. Moreover, the relative abundances of Fibrobacter spp. and Prevotellaceae UCG-003 increased, but those of Clostridium sensu stricto 1 and Corynebacterium 1 decreased in the LArg and HArg groups compared with those in the CON group. Additionally, the relative abundances of 19 OTUs were significantly different between the LArg and HArg groups. These results revealed that arginine supplementation affected the sika deer rumen bacterial community and serum biochemical indices.

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Vocalizations in red deer (Cervus elaphus), sika deer (Cervus nippon), and red × sika hybrids

Journal of Zoology ◽

10.1017/s0952836998001149 ◽

1998 ◽

Vol 244 (1) ◽

pp. 123-134 ◽

Cited By ~ 1

Author(s):

A. M. Long ◽

N.P. Moore ◽

T. J. Hayden

Keyword(s):

Cervus Elaphus ◽

Red Deer ◽

Sika Deer ◽

Cervus Nippon

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Improvement of Plasma Amino Acids During Fractionated Plasma Separation and Adsorption (Prometheus®)

Zeitschrift für Gastroenterologie ◽

10.1055/s-2006-931786 ◽

2006 ◽

Vol 44 (01) ◽

Author(s):

K Rifai ◽

A Das ◽

T Ernst ◽

U Kretschmer ◽

H Haller ◽

...

Keyword(s):

Amino Acids ◽

Plasma Amino Acids ◽

Plasma Separation

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Seasonal habitat selection of an expanding sika deer Cervus nippon population in eastern Hokkaido, Japan

Wildlife Biology ◽

10.2981/wlb.2003.036 ◽

2003 ◽

Vol 9 (1) ◽

pp. 141-153 ◽

Cited By ~ 23

Author(s):

Mayumi Sakuragi ◽

Hiromasa Igota ◽

Hiroyuki Uno ◽

Koichi Kaji ◽

Masami Kaneko ◽

...

Keyword(s):

Habitat Selection ◽

Sika Deer ◽

Cervus Nippon ◽

Selection Of

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Dietary Overlap But Spatial Gap between Sympatric Japanese Serow (Capricornis crispus) and Sika Deer (Cervus nippon) on Eastern Shikoku, Japan

Mammal Study ◽

10.3106/ms2018-0072 ◽

2019 ◽

Vol 44 (4) ◽

pp. 261

Author(s):

Asuka Yamashiro ◽

Yoshinori Kaneshiro ◽

Yoichi Kawaguchi ◽

Tadashi Yamashiro

Keyword(s):

Sika Deer ◽

Cervus Nippon ◽

Dietary Overlap ◽

Japanese Serow

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LEVELS OF FREE PLASMA AMINO ACIDS UNDER ACUTE NORMOBARIC HYPOXIA IN VOLUNTEERS IN FASTED AND POSTPRANDIAL STATES

Ulyanovsk Medico-biological Journal ◽

10.34014/2227-1848-2020-1-108-117 ◽

2020 ◽

pp. 108-117

Author(s):

A.A. Chernykh ◽

N.N. Potolitsyna ◽

E.A. Burykh ◽

E.R. Boyko

Keyword(s):

Amino Acids ◽

Normobaric Hypoxia ◽

Adaptation To Hypoxia ◽

Plasma Amino Acids ◽

Fed State ◽

Metabolic Substrates ◽

Overnight Fasting ◽

Group 2 ◽

Free Plasma ◽

Group 1

The aim of the study was to assess the effect of acute normobaric hypoxia on free plasma amino acids (AA) in volunteers after overnight fasting and in the fed state. Materials and Methods. Group 1 (n=13, aged 22–32) participated in the study in the morning after overnight fasting. Group 2 (n=9, aged 22–32) took part in the study after a light fat-free breakfast. Acute normobaric hypoxia was achieved by breathing a hypoxic gas mixture (9 % O2 and 91 % N2) through a mask. According to the experimental protocol, blood sampling from the cubital vein was performed for analysis. Free plasma amino acids were analyzed using the Aracus amino acid analyzer. Results. Prior to the hypoxia onset, at the 5th and 20th minutes of hypoxia, no statistically significant differences in free AA levels were observed in the groups (p>0.05). At the 10th minute of hypoxia the levels of four AAs (serine, threonine, glutamine, and histidine) were significantly higher in Group 1 than in Group 2 (p<0.05). This was probably due to differences in functioning of several key “harmonizing” AA transporters (ASCT1 (SLC1A4), ASCT2 (SLC1A5) and LAT1 (SC7A5)), for which the AAs were metabolic substrates. It can be assumed, that such changes were caused by currently unclear mechanisms of fast regulation of AA transporter activity, associated with nutritional status. Conclusion. We believe that our findings may be important for providing better adaptation to hypoxia, and for more efficient correction of hypoxic negative effects. Keywords: acute normobaric hypoxia, free plasma amino acids, human. Цель исследования: изучить воздействие острой нормобарической гипоксии на метаболизм свободных аминокислот (АК) плазмы крови у добровольцев, участвовавших в исследовании натощак и после лёгкого завтрака. Материалы и методы. Первая группа добровольцев (22–32 года, n=13) участвовала в исследовании утром натощак, вторая группа (22–32 года, n=9) – через 2–3 ч после лёгкого безжирового завтрака. Гипоксия создавалась путём подачи через маску дыхательной смеси, содержащей 9 % О2 и 91 % N2. В соответствии с протоколом проводился периодический забор крови из локтевой вены для анализа. Оценка уровней свободных АК плазмы крови производилась с помощью аминокислотного анализатора Aracus. Результаты. До начала гипоксии, на 5-й и 20-й мин гипоксии уровни свободных АК в первой и второй группах значимо не различались (p>0,05). На 10-й мин гипоксии между первой и второй группами наблюдались статистически значимые различия уровней четырёх АК: глутамина, серина, треонина и гистидина (p<0,05). Это, вероятно, было обусловлено изменениями в работе «гармонизирующих» мембранных транспортёров (ASCT1 (SLC1A4), ASCT2 (SLC1A5) и LAT1 (SC7A5)), для которых эти АК являются обменными субстратами. Можно предположить, что данные изменения были опосредованы пока неясными механизмами быстрой регуляции активности этих транспортёров, зависящими от питания. Выводы. Мы полагаем, что полученные результаты могут иметь значение для обеспечения адаптации организма человека к острой гипоксии и эффективной коррекции последствий гипоксического воздействия. Ключевые слова: острая нормобарическая гипоксия, свободные аминокислоты плазмы крови, человек.

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