biochemical indices
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2022 ◽  
Vol 28 (1) ◽  
pp. 46-49
Junbo Luo ◽  
Xuejun Li

ABSTRACT Introduction: Using gene therapy to transfer specific genes to implant therapeutic proteins into damaged tissues is a more promising way to treat sports injuries. The combination of tissue engineering and gene therapy will potentially promote the regeneration and repair of various damaged tissues. Objective: This article explores the adaptive relationship between gene selection therapy and athletes in sports. Methods: We selected students of related majors in sports schools to conduct specific genetic testing and measure the muscle area, fatigue level, muscle damage, and other related indicators before and after exercise. Results: After a series of physical fitness assessments, an increase in the gene sequence, as well as changes in the biochemical indices, were confirmed Conclusions: The muscle gain of the test subject during training is better than other genotypes. Level of evidence II; Therapeutic studies - investigation of treatment results.

Cells ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 277
Yichen Luo ◽  
Liang Du ◽  
Zhimeng Yao ◽  
Fan Liu ◽  
Kai Li ◽  

Chimeric RNAs (chiRNAs) play many previously unrecognized roles in different diseases including cancer. They can not only be used as biomarkers for diagnosis and prognosis of various diseases but also serve as potential therapeutic targets. In order to better understand the roles of chiRNAs in pathogenesis, we inserted human sequences into mouse genome and established a knockin mouse model of the tamoxifen-inducible expression of ASTN2-PAPPA antisense chimeric RNA (A-PaschiRNA). Mice carrying the A-PaschiRNA knockin gene do not display any apparent abnormalities in growth, fertility, histological, hematopoietic, and biochemical indices. Using this model, we dissected the role of A-PaschiRNA in chemical carcinogen 4-nitroquinoline 1-oxide (4NQO)-induced carcinogenesis of esophageal squamous cell carcinoma (ESCC). To our knowledge, we are the first to generate a chiRNA knockin mouse model using the Cre-loxP system. The model could be used to explore the roles of chiRNA in pathogenesis and potential targeted therapies.

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0259896
Qian Lin ◽  
Yang Liu ◽  
Simin Peng ◽  
Chunjie Liu ◽  
Tuo Lv ◽  

Magnolol is a bioactive polyphenolic compound commonly found in Magnolia officinalis. The aim of this study is to clarify the contribution of the magnolol additive on the growth performance of Linwu ducklings aging from 7 to 28 d, comparing to the effects of antibiotic additive (colistin sulphate). A total of 325, 7-d-old ducklings were assigned to 5 groups. Each group had 5 cages with 13 ducklings in each cage. The ducklings in different groups were fed with diets supplemented with 0, 100, 200 and 300 mg/kg magnolol additive (MA) (Control, MA100, MA200 and MA300) and 30 mg/kg colistin sulphate (CS30) for 3 weeks, respectively. Parameters regarding to the growth performance, intestinal mucosal morphology, serum biochemical indices, antioxidant and peroxide biomarkers and the expression levels of antioxidant-related genes were evaluated by one way ANOVA analysis. The results showed that 30 mg/kg colistin sulphate, 200 and 300 mg/kg magnolol additive improved the average final weight (P = 0.045), average daily body weight gain (P = 0.038) and feed/gain ratios (P = 0.001) compared to the control group. 200 and 300 mg/kg magnolol additive significantly increased the villus height/crypt depth ratio of ileum, compared to the control and CS30 groups (P = 0.001). Increased serum level of glucose (P = 0.011) and total protein (P = 0.006) were found in MA200 or MA300 group. In addition, comparing to the control and CS30 groups, MA200 or MA300 significantly increased the levels of superoxide dismutase (P = 0.038), glutathione peroxidase (P = 0.048) and reduced glutathione (P = 0.039) in serum. Moreover, the serum and hepatic levels of 8-hydroxy-2’-deoxyguanosine (P = 0.043 and 0.007, respectively) were lower in all MA groups compared to those of the control and CS30 group. The hepatic mRNA expression levels of superoxide dismutase-1, catalase and nuclear factor erythroid-2-related factor 2/erythroid-derived CNC-homology factor were also increased significantly in MA200 and MA300 groups (P < 0.05). Taken together, these data demonstrated that MA was an effective feed additive enhancing the growth performance of Linwu ducklings at 7 to 28 d by improving the antioxidant and intestinal mucosal status. It suggested that MA could be a potential ingredient to replace the colistin sulphate in diets.

2021 ◽  
Vol 23 (104) ◽  
pp. 55-59
N. Cherniy ◽  
I. Skvortsova ◽  
B. Gutyj ◽  
R. Mylostyvyi ◽  
V. Voronyak

The results of the studies on the determination of the optimal dose of the probiotic additive “Evitalia” (PAE) for the quails of the Texas breed have been presented in the article. The experiments were conducted on the quails aged 1–35 days. The object of the research was the probiotic additive “Evitalia” that consisted of eight components (the strains of lactic acidmicroorganisms, macro- and microelements). The subject of the investigation was the influence of PAE on the growth, preservation, the morphological composition and biochemical indices of blood. The studies were carried out in the following conditions of microclimate and sanitary regime: the air temperature in the pens-cages was 16–18 ºC in the autumn and winter period that was 5–7 ºC lower than it was provided by the standard; in spring the temperature was 23–15 ºС that was lower than it was provided by the standard (29–35 ºС). The concentration of carbon dioxide ranged within 0.20–0.25 % (2.0–2.5 l/m3), ammonia – 12–13.4 mg/m3, the general contamination of the air by microflora was 50–60 thousand KOE/m3. The experiments were conducted on the four groups of quails, 60 quails in each group. The quails of the control group were fed by water without PAE, the probiotic at the dose of 7 mg/500 ml of water was given to the quails of the experimental group 1 (E-1), 9 mg of probiotic/500 ml water received the birds of the experimental group 2 (E-2) and the quails of the experimental group 3 (E-3) were given the probiotic at the dose of 11 mg/500 ml water. The positive influence of the probiotic additive “Evitalia” on the growth and the body live weight gain of the quails was observedin the experimental groups as compared to the quails of the control group. The trustworthy increase in the growth and live weight gain of the quails was detected on the 7-, 14-, 21-, 35-day of their life (Р < 0.05), especially in the quails that received PAE at the dose of 9 mg (E-2). The quails of the experimental group 2 exceeded the others by the average daily live weight gains. The growth intensity of the quails in the experimental group 2 was 6.43 % higher than that of the control one, higher by 3.47 % and 5.57 % than in the experimental groups 1 and 3, respectively. The preservation of the quails in the control group was lower by 4.8 – 5.1 % as compared to the quails in the experimental groups. By the data on the growth of the body live weight, the composition and biochemical indices of blood of the quails it can be concluded that the optimal dose of the probioticadditive “Evitalia” is 9 mg/500 ml water that activates hematopoesis and stimulates the growth and development of quails.

2021 ◽  
Vol 12 ◽  
Jing Chen ◽  
Saizhen Chen ◽  
Jinguang Chen ◽  
Bixin Shen ◽  
Zhengli Jiang ◽  

Atopic dermatitis (AD) is a common chronic relapsing skin inflammation, which severely affect the quality of life of patients. Inhibiting itching and enhancing immunity to mitigate scratching are key elements in the fight against AD. Huanglian Jiedu decoction (HLJDD) has multiple pharmacological effects in the treatment of AD. However, the effective ingredients and underlying molecular mechanisms have not yet been fully explored. Thus, this study integrates chemistry, biochemistry, and metabolomics strategies to evaluate the active substance basis of HLJDD against AD. First, HLJDD was split to five fractions (CPF, 40AEF, 90AEF, PEF and WEF) and 72 chemical components were identified. NSD (Non-similarity degree) among the different fractions showed significant chemical differences (&gt;81%). Interleukin IL-13, IL-17A, IL-3, IL-31, IL-33, IL4, IL-5, TSLP, IgE, and histamine in the serum, and IL-4Rα, JAK1, and HRH4 levels in skin, participating in inhibiting itching and regulating immunity signaling, were found to be restored to varying degrees in AD treating with HLJDD and its fractions, especially 40AEF and CPF. Untargeted metabolomics analysis demonstrated that forty metabolites were differential metabolites in plasma between the HLJDD-treated group and the AD group, involving in histidine metabolism, arginine biosynthesis, pyrimidine metabolism, and so on. Further, targeted metabolomics analysis revealed that eleven differential metabolites, associating with physiological and biochemical indices, were significant improved in the HLJDD and its fractions groups. In conclusion, HLJDD exhibited anti-AD effects by inhibiting itching and enhancing immunity, which in turn regulating the levels of relative metabolites, and CPF and 40AEF were considered the most important components of HLJDD.

F. B. P. Abang ◽  
S. S. Emmanuel ◽  
S. Attah

Twenty five (25) mixed breed weaned rabbits were utilized in 84-days experiment to investigate the effect of replacing bone ash with fresh water snail (Pila ampullacea) shell ash on the serum biochemical composition of weaned rabbits. The experiment was conducted at the Teaching and Research Farm, University of Agriculture, Makurdi, Benue State, Nigeria for a period of 12 weeks. The rabbits were randomly assigned to the five dietary treatments and replicated five times giving a total of one rabbit per replicate in a completely randomized design (CRD).   Rabbits were raised intensively in hutches of about 4ft × 4ft. Five experimental diets tagged T1 to T5 were formulated  such that fresh water snail (Pila ampullacea) shell ash replaced bone ash at 0%, 25%, 50%, 75% and 100%. Feed and water were served ad libitum. Bio sanitary and bio security measures were strictly adhered to. At the end of the feeding trial, three rabbits per treatment were selected for the evaluation of serum biochemistry. The parameters assayed for were: total serum protein, serum globulin, serum albumin, cholesterol, serum calcium, and serum phosphorus. The study showed that the serum biochemical indices were not influenced (P˃0.05) by the dietary treatments except for calcium which was significantly (P<0.05) influenced, however, calcium values were within the normal reference values for rabbits. This study had shown that fresh water snail (Pila ampullacea) shell ash can serve as a substitute for bone ash in weaned rabbit diet up to 100% inclusion levels without adverse effect on the serum biochemical indices.

A.I. Afanasyeva ◽  
V.A. Sarychev ◽  
S.N. Chebakov ◽  

The seed bulls used for artificial insemination should be in good health, have outstanding genetic characteristics, have high sexual activity, and the biological products ob-tained from them should have high quality indices. One of the possible methods of increasing semen production is the introduction of dietary supplements into the diet. The intro-duction of new feed and biologically active supplements into the diet may cause both positive and negative changes in metabolic processes. Monitoring the biochemical status of blood allows detecting not only metabolic disorders in highly productive animals but also a lack of vitamins, mac-ro-and microelements. The research goal was to study the effect of a natural biostimulant (mineral supplement) made from maral antler press-cake on the blood biochemical indices ofBlack Pied seed bulls in the breeding company AO “Plempredpriyatiye Barnaulskoye”. Along with the basic diet, the seed bulls of the trial group received the natural biostimulant (mineral supplement) based on maral antler press-cake; the daily dose -15.0 g per head, for 30 days with 10 days’ interval after each application. The supple-ment was thoroughly mixed with compound feed before feeding. The mineral supplement is of powder form and contains a complex of vitamins, minerals, amino acids and other biologically active substances. The use of a mineral supplement increased the amount of total protein and al-bumin by 7.15% and 18.3%, respectively. The blood glu-cose level in the seed bulls of the trial group was higher by 12% and cholesterol by 17.1% than in the blood of the con-trol group animals. Cholesterol of the seed bulls may be used as a structural material or a precursor in the synthesis of sex steroid hormones. The level of total calcium, alkaline reserve at the end of the experiment increased in the seed bulls when using the mineral supplement made from maral antler press-cake in the diet by 4.8% and 8.1%, respective-ly, as compared to the control group.

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3425
Li Gong ◽  
Gengsheng Xiao ◽  
Liwei Zheng ◽  
Xia Yan ◽  
Qien Qi ◽  

This study aimed to evaluate the effects of tributyrin on growth performance, biochemical indices and intestinal microbiota of yellow-feathered broilers. 360 one-day-old chicks were randomly allocated to three treatments with six replicates of 20 chicks each, including a normal control group (NC), an antibiotic group (PC), and a tributyrin (250 mg/kg) group (TB) for 63 days. The results showed that compared with the control, the feed conversion ratio (FCR) in the TB group decreased during the d22 to d42 (p < 0.05) and overall, the final weight and FCR of broilers tended to increase and decrease, respectively. Moreover, the TB group showed the highest creatine concentrations at the entire period (p < 0.05). TB treatment increased the Bacteroidetes relative abundance and decreased Firmicutes. Principal coordinates analysis yielded clear clustering of the three groups. Linear discriminant analysis effect size analysis found seven differentially abundant taxa in the TB group, including several members of Bacteroidedetes. The relative abundance of Eisenbergiella, Phascolarctobacterium, Megasphaera and Intestinimonas increased in tributyrin-treated broilers. Spearman correlation analysis identified a correlation between Eisenbergiella abundance and overall feed efficiency. These results demonstrated that tributyrin could improve the growth performance by modulating blood biochemical indices and the cecal microflora composition of broilers.

2021 ◽  
Vol 11 (1) ◽  
Wen-Ti Lin ◽  
Kuen-Cheh Yang ◽  
Yen-Ting Chen ◽  
Kuo-Chin Huang ◽  
Wei-Shiung Yang

AbstractS14 has been identified as a potent stimulator of de novo hepatic lipogenesis (DNL) in rodents. However, it is unclear how S14 is regulated in humans with non-alcoholic fatty liver disease (NAFLD). The aim of this study was to investigate the relationship between serum S14 and liver steatosis in humans with NAFLD. A total of 614 participants were recruited from community. Liver steatosis were evaluated according to the Ultrasonographic Fatty Liver Indicator (US-FLI), which is a semi-quantitative liver ultrasound score. Anthropometric and biochemical indices were collected for further analysis. The risk of liver steatosis severity was estimated by a cumulative logistic regression model. NAFLD was found in 52.2% of the participants. The subjects with NAFLD showed higher levels of waist circumference, body mass index, insulin resistance, aspartate aminotransferase, dyslipidemia, visceral fat, serum S14 and risk of metabolic syndrome (MetS) than those of controls. Compared with the first tertile of serum S14, the odds ratios for the risk of more severe liver steatosis were 1.22 (95% confidence interval [CI]: 0.78–1.92) for those of the second tertile and 2.08 (95% CI: 1.28–3.39) for the third tertile (P for trend < 0.05) after adjusting for confounding factors. Higher serum S14 level was not only found in NAFLD subjects but also was positively correlated with the severity of liver steatosis. S14 may play an important role in the mechanism of DNL for NAFLD in humans.

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