scholarly journals Transmissible gastroenteritis virus: Identification of M protein-binding peptide ligands with antiviral and diagnostic potential

2013 ◽  
Vol 99 (3) ◽  
pp. 383-390 ◽  
Author(s):  
Hao Zou ◽  
Dante S. Zarlenga ◽  
Karol Sestak ◽  
Siqingaowa Suo ◽  
Xiaofeng Ren
Keyword(s):  
Protein Binding ◽  
Peptide Ligands ◽  
M Protein ◽  
Transmissible Gastroenteritis Virus ◽  
Binding Peptide ◽  
Virus Identification ◽  
Gastroenteritis Virus ◽  
Diagnostic Potential ◽  
Transmissible Gastroenteritis

scholarly journals Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential

Virus Genes ◽  
2015 ◽  
Vol 51 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Siqingaowa Suo ◽  
Xue Wang ◽  
Dante Zarlenga ◽  
Ri-e Bu ◽  
Yudong Ren ◽  
...  
Keyword(s):  
Phage Display ◽  
Spike Protein ◽  
Transmissible Gastroenteritis Virus ◽  
Gastroenteritis Virus ◽  
Diagnostic Potential ◽  
Transmissible Gastroenteritis

scholarly journals Reverse Genetic Analysis of the Transcription Regulatory Sequence of the Coronavirus Transmissible Gastroenteritis Virus

2004 ◽  
Vol 78 (11) ◽  
pp. 6061-6066 ◽  
Author(s):  
Kristopher M. Curtis ◽  
Boyd Yount ◽  
Amy C. Sims ◽  
Ralph S. Baric
Keyword(s):  
Genetic Analysis ◽  
Full Length ◽  
Transmissible Gastroenteritis Virus ◽  
Regulatory Sequence ◽  
Reverse Genetic ◽  
Conserved Sequence ◽  
Gastroenteritis Virus ◽  
Flanking Sequences ◽  
Transmissible Gastroenteritis ◽  
Discontinuous Transcription

ABSTRACT Coronavirus discontinuous transcription uses a highly conserved sequence (CS) in the joining of leader and body RNAs. Using a full-length infectious construct of transmissable gastroenteritis virus, the present study demonstrates that subgenomic transcription is heavily influenced by upstream flanking sequences and supports a mechanism of transcription attenuation that is regulated in part by a larger domain composed of primarily upstream flanking sequences which select appropriately positioned CS elements for synthesis of subgenomic RNAs.

scholarly journals The Application of Immunogold Silver Staining (IGSS) for the Detection of Transmissible Gastroenteritis Virus in Fixed Tissues

1993 ◽  
Vol 5 (1) ◽  
pp. 16-20 ◽  
Author(s):  
Renke Larochelle ◽  
Ronald Magar
Keyword(s):  
Silver Staining ◽  
Protein A ◽  
Immunogold Electron Microscopy ◽  
Transmissible Gastroenteritis Virus ◽  
Tissue Sections ◽  
Gastroenteritis Virus ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Transmissible Gastroenteritis ◽  
Immune Aggregates

Protein A-gold (PAG) and a primary porcine antiserum were used in immunogold silver staining (IGSS) for the detection of transmissible gastroenteritis virus (TGEV) in formalin-fixed paraffin-embedded tissue sections of small intestine originating from infected pigs. Immunogold electron microscopy was used to evaluate the reactivity of the prepared PAG marker with the specific porcine TGEV antiserum. Gold particles were closely associated with single virions and immune aggregates of TGEV. When IGSS, using PAG as the marker, was applied to tissue sections, dark staining of TGEV-infected villous enterocytes was observed. Background was low, allowing good visualization by light microscopy of the distribution of viral antigen. Two other gold conjugates, protein A/G-gold (PA/GG) and protein G-gold (PGG), were tested in IGSS. The labeling with PA/GG was comparable to that obtained with PAG. However, no staining was observed when PGG was used. The use of IGSS and PAG offers advantages and may represent a useful technique for the detection of other viral pathogens.

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