Abstract. In mining areas in which sulfur-containing ores are exposed, the oxidation of sulfides leads to the formation of acidic mine drainage (AMD) waters. Both traditional and molecular microbial studies have shown that chemolithotrophic sulfur- and iron-oxidizing bacteria are responsible for this activity. The Iberian Pyrite Belt (IPB), located in southwestern Spain represents one of the world’s largest accumulations of mine wastes and AMD waters. Mineralogical and textural characteristics of the IPB ores favor the oxidation and dissolution of pyrite and the subsequent formation of AMD waters. Acidic pit lakes in the IPB are a hotspot for this activity. These pit lakes are former open pit mines where ores have been exposed leading the formation of AMD waters. At present, there are more than 25 of these pit lakes between the provinces of Huelva and Seville (SW Spain). This work reports the physical properties, hydrogeochemical characteristics, and microbial diversity of two pit lakes located in the IPB Nuestra Señora del Carmen (NSC), and Concepción (CN). Both pit lakes are acid (pH 2-4) and showed chemical and thermal stratification with well defined chemoclines. One particular characteristic of NSC is that it has developed a chemocline very close to the surface (2 m depth). Microbial community composition of the water column was analyzed by 16S and 18S rRNA gene cloning and sequencing. The microorganisms detected in NSC are characteristic of acid mine drainage (AMD), including iron oxidizing bacteria (Leptospirillum) and facultative iron reducing bacteria and archaea (Acidithiobacillus ferrooxidans, Actinobacteria, Acidimicrobiales, Ferroplasma) detected in the bottom layer. Diversity in CN was higher than in NSC. Microorganisms known from AMD systems (Acidiphilium, Acidobacteria and Ferrovum) and microorganisms never reported from AMD systems were both identified.
Microbial Diversity and Its Relationship to Physicochemical Characteristics of the Water in Two Extreme Acidic Pit Lakes from the Iberian Pyrite Belt (SW Spain)