A kit for labeling of [188Re] human serum albumin microspheres for therapeutic use in nuclear medicine

In nuclear medicine, radiation absorbed dose estimates calculated by standard models at the whole body or organ are very low. At cellular level, however, the heterogeneity of radionuclide distributions of radiation dose patterns may be significant. We present here absorbed doses at cellular level and evaluate their possible impact on the usually assumed risk/benefit relationships in nuclear medicine studies. The absorbed dose values calculated are surprisingly high, and are difficult to interpret. In the present study, we show calculated doses at the cellular level and discuss possible biological consequences, for two radiopharmaceuticals labelled with technetium-99m: human serum albumin microspheres used for pulmonary scintigrapies and HMPAO used to labelled leukocytes.

Download Full-text

The Radiopharmacist in Nuclear Medicine: Preparation of Technetium-99m Human Serum Albumin Macroaggregates

American Journal of Health-System Pharmacy ◽

10.1093/ajhp/26.9.529 ◽

1969 ◽

Vol 26 (9) ◽

pp. 529-534

Author(s):

Barry M. Bowen ◽

Donald E. Wood

Keyword(s):

Nuclear Medicine ◽

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Technetium 99M

Download Full-text

Human SeruM Albumin Microspheres Approximate Initial Organ-Specific Biodistributions of Transplanted Hepatocytes and Are Effective Cell Surrogates for Safety Studies

Cell Transplantation ◽

10.1177/096368979800700306 ◽

1998 ◽

Vol 7 (3) ◽

pp. 275-283 ◽

Cited By ~ 3

Author(s):

Pankaj Rajvanshi ◽

Kuldeep K. Bhargava ◽

Menes Afriyie ◽

Maria V. Camaya ◽

S. Gagandeep ◽

...

Keyword(s):

Human Serum Albumin ◽

Serum Albumin ◽

Human Serum ◽

Femoral Vein ◽

Rat Hepatocytes ◽

Hepatocyte Transplantation ◽

Albumin Microspheres ◽

Liver Repopulation ◽

Organ Specific ◽

Safety Studies

Liver repopulation with transplanted hepatocytes will generate novel cell-based therapies, although translocation of transplanted cells into lungs through portasystemic shunts has the potential for embolic complications. To facilitate safety analysis of hepatocyte transplantation, we wished to obtain effective cell surrogates and analyzed biodistributions of similarly sized 99mTc-labeled human serum albumin microspheres and rat hepatocytes. Image analysis with dual 99mTc and 111In labels indicated that cells and microspheres were similarly distributed in the liver when injected into normal rats via the spleen. Also, their distributions were similar when injected via a femoral vein or the superior mesenteric vein with cells and microspheres localizing in lungs or liver, respectively. Upon intraportal injection in rats with portal hypertension, microspheres localized in both liver and lungs, consistent with portasystemic shunting. These data demonstrate that human serum albumin microspheres are effective cell surrogates for approximating the safety of hepatocyte transplantation and should be clinically useful.

Download Full-text