Genetic and phylogenetic analysis of the honey bee sacbrood virus from jiangxi isolates

2022 ◽  
Vol 25 (1) ◽  
pp. 101847
Author(s):  
Yaping Meng ◽  
Xinyue Yu ◽  
Qiang Huang ◽  
Lizhen Zhang ◽  
Xiaobo Wu ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Honey Bee ◽  
Sacbrood Virus

Dynamics of Apis cerana Sacbrood Virus (AcSBV) Prevalence in Apis cerana (Hymenoptera: Apidae) in Northern Taiwan and Demonstration of its Infection in Apis mellifera (Hymenoptera: Apidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2055-2066 ◽  
Author(s):  
Chong-Yu Ko ◽  
Zong-Lin Chiang ◽  
Ruo-Jyun Liao ◽  
Zih-Ting Chang ◽  
Ju-Chun Chang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Mellifera ◽  
Honey Bee ◽  
Apis Cerana ◽  
Cross Infection ◽  
Prevalence Rates ◽  
Sacbrood Virus ◽  
Northern Taiwan ◽  
Long Term Survey

AbstractSince 2016, Apis cerana sacbrood virus (AcSBV) has been recorded in Taiwan. It is epizootic in Apis cerana (Hymenoptera: Apidae) and causing serious loss of A. cerana. Herein, we performed a long-term survey of AcSBV prevalence in the populations of A. cerana in Northern Taiwan from January 2017 to July 2018. The surveillance of AcSBV prevalence in A. mellifera (Hymenoptera: Apidae) populations was starting and further confirmed by sequencing since April 2017; thus, these data were also included in this survey. In our survey, the average prevalence rates of AcSBV were 72 and 53% in A. cerana and A. mellifera, respectively, in 2017, which decreased to 45 and 27% in 2018. For the spatial analysis of AcSBV in two honey bee populations, Hsinchu showed the highest prevalence, followed by New Taipei, Yilan, Taipei, and Keelung, suggesting that AcSBV might have come from the southern part of Taiwan. Interestingly, the AcSBV prevalence rates from A. cerana and A. mellifera cocultured apiaries gradually synchronized. The result of phylogenetic analysis and comparison of the annual AcSBV prevalence in A. cerana-only, A. mellifera-only, and A. cerana/A. mellifera cocultured sample sites indicate cross-infection between A. cerana and A. mellifera; however, AcSBV may lose the advantage of virulence in A. mellifera. The evidence suggested that the transmission of AcSBV might occur among these two honey bee species in the field. Therefore, A. mellifera may serve as a guard species to monitor AcSBV in A. cerana, but the cross-infection still needs to be surveyed.

scholarly journals Complete genome sequence of sacbrood virus isolated from Asiatic honey bee Apis cerana indica in India

VirusDisease ◽  
2018 ◽  
Vol 29 (4) ◽  
pp. 453-460 ◽  
Author(s):  
R. Aruna ◽  
M. R. Srinivasan ◽  
V. Balasubramanian ◽  
R. Selvarajan
Keyword(s):  
Honey Bee ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Apis Cerana ◽  
Sacbrood Virus ◽  
Apis Cerana Indica

scholarly journals Molecular detection and phylogenetic analysis of the honeybee (Apismellifera) sacbrood virus in Turkey

2019 ◽  
Vol 43 (4) ◽  
pp. 551-554 ◽  
Author(s):  
Gülnur KALAYCI ◽  
Abdurrahman Anıl ÇAĞIRGAN ◽  
Kemal PEKMEZ ◽  
Buket ÖZKAN ◽  
Murat KAPLAN
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Detection ◽  
Sacbrood Virus

Phylogenetic analysis and survey of Apis cerana strain of Sacbrood virus (AcSBV) in Taiwan suggests a recent introduction

2017 ◽  
Vol 146 ◽  
pp. 36-40 ◽  
Author(s):  
Wei-Fone Huang ◽  
Shahid Mehmood ◽  
Shaokang Huang ◽  
Yue-Wen Chen ◽  
Chong-Yu Ko ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Cerana ◽  
Sacbrood Virus ◽  
Recent Introduction

Sacbrood virus of the larval honey bee (Apis mellifera linnaeus)

Virology ◽  
1964 ◽  
Vol 23 (3) ◽  
pp. 425-429 ◽  
Author(s):  
L. Bailey ◽  
A.J. Gibbs ◽  
R.D. Woods
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Sacbrood Virus

scholarly journals Sacbrood Virus of the Honeybee (Apis mellifera): Rapid Identification and Phylogenetic Analysis Using Reverse Transcription-PCR

2001 ◽  
Vol 8 (1) ◽  
pp. 93-104 ◽  
Author(s):  
Elvira Grabensteiner ◽  
Wolfgang Ritter ◽  
Michael J. Carter ◽  
Sean Davison ◽  
Hermann Pechhacker ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Apis Mellifera ◽  
Reverse Transcription ◽  
Direct Detection ◽  
Geographic Origin ◽  
Rapid Identification ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Sacbrood Virus ◽  
Reverse Transcription Pcr

ABSTRACT Sacbrood virus (SBV) infects larvae of the honeybee (Apis mellifera), resulting in failure to pupate and death. Until now, identification of viruses in honeybee infections has been based on traditional methods such as electron microscopy, immunodiffusion, and enzyme-linked immunosorbent assay. Culture cannot be used because no honeybee cell lines are available. These techniques are low in sensitivity and specificity. However, the complete nucleotide sequence of SBV has recently been determined, and with these data, we now report a reverse transcription-PCR (RT-PCR) test for the direct, rapid, and sensitive detection of these viruses. RT-PCR was used to target five different areas of the SBV genome using infected honeybees and larvae originating from geographically distinct regions. The RT-PCR assay proved to be a rapid, specific, and sensitive diagnostic tool for the direct detection of SBV nucleic acid in samples of infected honeybees and brood regardless of geographic origin. The amplification products were sequenced, and phylogenetic analysis suggested the existence of at least three distinct genotypes of SBV.

scholarly journals Occurrence and Phylogenetic Analysis of DWV in Stingless Bee (Apidae sp.) in China: A Case Report

2021 ◽  
Vol 1 ◽  
Author(s):  
Lina Zhang ◽  
Yanchun Deng ◽  
Hongxia Zhao ◽  
Ming Zhang ◽  
Chunsheng Hou
Keyword(s):  
Phylogenetic Analysis ◽  
Honey Bee ◽  
Honey Bees ◽  
Stingless Bees ◽  
Vital Role ◽  
Stingless Bee ◽  
Pollination Services ◽  
Important Indicator ◽  
Bee Colonies ◽  
Bee Viruses

Honey bees play a vital role in providing pollination services for agricultural crops and wild flowering plants. However, the spillover risk of their pathogens to other pollinators or wild insects is becoming a cause for concern. There is some evidence that stingless bees can carry honey bee viruses, but little is known about the presence of honey bee viruses in stingless bees in China. Here, we investigate the occurrence of major honey bee pathogens including bacteria, fungi, and viruses in stingless bees (Apidae: sp.). Our results show that the stingless bees (Apidae: sp.) were mainly infected with DWV-A, but no DWV-B and DWV-C. Phylogenetic analysis on fragments of lp, RdRp, and VP3 of DWV-A indicated that genetic variation in VP3 might an important indicator for host-specific viruses, but it requires further study. Our results indicated that DWV-A is not only the major strain of virus currently circulating in managed bee colonies in China and globally, but in stingless bee species as a whole.

In-situ Hybridization for the Detection of Sacbrood Virus in Infected Larvae of the Honey Bee (Apis cerana)

2016 ◽  
Vol 154 (2-3) ◽  
pp. 258-262 ◽  
Author(s):  
C. Park ◽  
H.S. Kang ◽  
J. Jeong ◽  
I. Kang ◽  
K. Choi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Honey Bee ◽  
Apis Cerana ◽  
Sacbrood Virus
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