Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity

2016 ◽  
Vol 1861 (9) ◽  
pp. 1365-1378 ◽  
Author(s):  
Ahmed M. Abd-El-Haliem ◽  
Jack H. Vossen ◽  
Arjan van Zeijl ◽  
Sara Dezhsetan ◽  
Christa Testerink ◽  
...  
Keyword(s):  
Phospholipase C ◽  
Biochemical Characterization ◽  
Plant Immunity

Uronema marinum: Identification and biochemical characterization of phosphatidylcholine-hydrolyzing phospholipase C

2005 ◽  
Vol 110 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Jung Soo Seo ◽  
Moo Sang Kim ◽  
Sang Hwan Lee ◽  
Ki Hong Kim ◽  
Hyung Ho Lee ◽  
...  
Keyword(s):  
Phospholipase C ◽  
Biochemical Characterization

Pic1, counteracting plant immunity signalling

2019 ◽  
Vol 476 (16) ◽  
pp. 2347-2350
Author(s):  
Saijaliisa Kangasjärvi
Keyword(s):  
Biochemical Characterization ◽  
Plant Immunity ◽  
Cell Receptor ◽  
Central Position ◽  
Signalling Network ◽  
Biochemical Journal ◽  
Defence Signalling ◽  
Pathogenic Microbes ◽  
Defensive Measures

Abstract Plants are equipped with versatile pattern recognition receptors (PRRs), which monitor their external environment and elicit defensive measures upon detection of potential risk for disease. Inside the cell, receptor-like cytoplasmic kinases (RLCKs) are key components of PRR signalling, but their molecular functions and regulatory interactions are not yet fully understood. In tomato, two RLCKs, Pti1a and Pti1b, are important signalling components that relay early defence signals elicited by bacterial flagellin, a conserved pattern common to various pathogenic and non-pathogenic microbes. An important question to resolve is how plant immune reactions are regulated to prevent unnecessary defensive measures. A recent paper published in the Biochemical Journal by Giska and Martin [Biochem. J. (2019) 476, 1621–1635] reports the identification and biochemical characterization of a new tomato (Solanum lycopersicum) protein phosphatase that negatively controls early defence signalling. The phosphatase, termed pattern-triggered immunity inhibiting PP2C 1 (Pic1), negatively controls the signalling function of Pti1b and therefore holds a central position in the defence signalling network. The Pti1b–Pic1 kinase–phosphatase interaction provides mechanistic insights that forward our understanding of protein phosphatases and their importance in plant immunity.

Plant Pathology Diagnosed by X-Ray Microanalysis

1987 ◽  
Vol 45 ◽  
pp. 974-975
Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang
Keyword(s):  
Electron Microscopy ◽  
Plant Pathology ◽  
Biochemical Characterization ◽  
Nutrient Deficiency ◽  
Green Leaf ◽  
Parasitic Infestation ◽  
X Ray

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.

Interaction effect of rootstocks on gas exchange parameters, biochemical changes and nutrientstatus in Sauvignon Blanc winegrapes

2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke
Keyword(s):  
Biochemical Characterization ◽  
Reducing Sugar ◽  
Wine Grape ◽  
Gas Exchange Parameters ◽  
Food Material ◽  
Total Carbohydrates ◽  
Salt Creek ◽  
Grape Varieties ◽  
Photosynthetic Activities

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.

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