Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance

Xiaoqiu Wu; Hans Jörnvall; Kurt D Berndt; Udo Oppermann

doi:10.1016/j.bbrc.2003.11.091

Codon optimization reveals critical factors for high level expression of two rare codon genes in Escherichia coli: RNA stability and secondary structure but not tRNA abundance

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2003.11.091 ◽

2004 ◽

Vol 313 (1) ◽

pp. 89-96 ◽

Cited By ~ 70

Author(s):

Xiaoqiu Wu ◽

Hans Jörnvall ◽

Kurt D Berndt ◽

Udo Oppermann

Keyword(s):

Escherichia Coli ◽

Secondary Structure ◽

Codon Optimization ◽

Rna Stability ◽

Rare Codon ◽

Critical Factors ◽

High Level Expression ◽

High Level

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Effects of rare codon clusters on high-level expression of heterologous proteins in Escherichia coli

Current Opinion in Biotechnology ◽

10.1016/0958-1669(95)80082-4 ◽

1995 ◽

Vol 6 (5) ◽

pp. 494-500 ◽

Cited By ~ 453

Author(s):

James F Kane

Keyword(s):

Escherichia Coli ◽

Rare Codon ◽

Heterologous Proteins ◽

High Level Expression ◽

High Level

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High-level expression of Falcipain-2 in Escherichia coli by codon optimization and auto-induction

Protein Expression and Purification ◽

10.1016/j.pep.2012.03.008 ◽

2012 ◽

Vol 83 (1) ◽

pp. 59-69 ◽

Cited By ~ 14

Author(s):

Emir Salas Sarduy ◽

Aymara Cabrera Muñoz ◽

Sebastián Alejandro Trejo ◽

María de los A. Chavéz Planes

Keyword(s):

Escherichia Coli ◽

Codon Optimization ◽

High Level Expression ◽

High Level

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Codon optimization for high level expression of human bone morphogenetic protein – 2 in Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2012.05.010 ◽

2012 ◽

Vol 84 (2) ◽

pp. 188-194 ◽

Cited By ~ 8

Author(s):

Debbie S. Retnoningrum ◽

H.T. Pramesti ◽

P.Y. Santika ◽

O. Valerius ◽

S. Asjarie ◽

...

Keyword(s):

Escherichia Coli ◽

Bone Morphogenetic Protein ◽

Codon Optimization ◽

Human Bone ◽

Bone Morphogenetic Protein 2 ◽

High Level Expression ◽

Morphogenetic Protein ◽

Human Bone Morphogenetic Protein ◽

High Level

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High-Level Expression of Porcine Liver Cytochrome P-450 Reductase Catalytic Domain in Escherichia coli by Modulating the Predicted Local Secondary Structure of mRNA

The Journal of Biochemistry ◽

10.1093/jb/mvg158 ◽

2003 ◽

Vol 134 (3) ◽

pp. 403-413 ◽

Cited By ~ 15

Author(s):

S. Kimura

Keyword(s):

Escherichia Coli ◽

Secondary Structure ◽

Catalytic Domain ◽

High Level Expression ◽

Porcine Liver ◽

Liver Cytochrome ◽

Local Secondary Structure ◽

High Level ◽

Cytochrome P 450

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High-Level Expression of Prochymosin in Escherichia coli: Effect of the Secondary Structure of the Ribosome Binding Site

Protein Expression and Purification ◽

10.1006/prep.1995.1037 ◽

1995 ◽

Vol 6 (3) ◽

pp. 284-290 ◽

Cited By ~ 17

Author(s):

G. Wang ◽

N.J. Liu ◽

K.Y. Yang

Keyword(s):

Escherichia Coli ◽

Secondary Structure ◽

Binding Site ◽

Ribosome Binding Site ◽

High Level Expression ◽

Ribosome Binding ◽

High Level

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Cloning and high level expression of Cynodon dactylon (Bermuda grass) pollen profilin (Cyn d 12) in Escherichia coli: purification and characterization of the allergen

Clinical & Experimental Allergy ◽

10.1046/j.1365-2222.1997.1470952.x ◽

1997 ◽

Vol 27 (11) ◽

pp. 1307-1313 ◽

Cited By ~ 2

Author(s):

J. A. ASTURIAS ◽

M. C. ARILLA ◽

N. GOMEZ-BAYON ◽

J. MARTINEZ ◽

A. MARTINEZ ◽

...

Keyword(s):

Escherichia Coli ◽

Grass Pollen ◽

Cynodon Dactylon ◽

Bermuda Grass ◽

Purification And Characterization ◽

High Level Expression ◽

Bermuda Grass Pollen ◽

D 12 ◽

High Level

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Polyoma virus major capsid protein, VP1. Purification after high level expression in Escherichia coli.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)38948-2 ◽

1985 ◽

Vol 260 (23) ◽

pp. 12803-12809

Author(s):

A D Leavitt ◽

T M Roberts ◽

R L Garcea

Keyword(s):

Escherichia Coli ◽

Capsid Protein ◽

Major Capsid Protein ◽

Polyoma Virus ◽

High Level Expression ◽

Expression In Escherichia Coli ◽

Capsid Protein Vp1 ◽

High Level

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High-level expression of a novel multifunctional GH3 family β-xylosidase/α-arabinosidase/β-glucosidase from Dictyoglomus turgidum in Escherichia coli

Bioorganic Chemistry ◽

10.1016/j.bioorg.2021.104906 ◽

2021 ◽

pp. 104906

Author(s):

Xinyi Tong ◽

Zhipeng Qi ◽

Daiyi Zheng ◽

Jianjun Pei ◽

Qi Li ◽

...

Keyword(s):

Escherichia Coli ◽

High Level Expression ◽

Dictyoglomus Turgidum ◽

High Level

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High level expression of human lipocortin (annexin) 1 in Escherichia coli

Biotechnology Letters ◽

10.1007/bf00128673 ◽

1996 ◽

Vol 18 (2) ◽

pp. 163-168 ◽

Cited By ~ 4

Author(s):

Kwang-Rae Huh ◽

Eun Hee Cho ◽

Soo Ok Lee ◽

Doe Sun Na

Keyword(s):

Escherichia Coli ◽

High Level Expression ◽

Annexin 1 ◽

High Level

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High-level expression of a functional human fibrinogen gamma chain in Escherichia coli

Gene ◽

10.1016/0378-1119(88)90355-1 ◽

1988 ◽

Vol 66 (2) ◽

pp. 183-192 ◽

Cited By ~ 23

Author(s):

Mark G. Bolyard ◽

Susan T. Lord

Keyword(s):

Escherichia Coli ◽

High Level Expression ◽

Gamma Chain ◽

Human Fibrinogen ◽

High Level

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